Results 251 to 260 of about 1,770,911 (287)

Real-Time PCR

2015
Wie bereits unter Kap. 13 erwahnt, ist die Quantifizierung der amplifizierten PCR-Produkte nach 25–30 Zyklen nicht mehr sehr einfach. Damit eine Quantifizierung der zu untersuchenden Amplicons (ca. 50–150 bp Lange) ermoglicht werden kann, haben sich verschiedene optische PCR-Systeme zur „online“ Beobachtung des Amplifikationsstatus etabliert (Tab. 14.1)
Hans-Joachim Müller, Daniel Ruben Prange   +1 more
openaire   +2 more sources

Real-Time Multiplex PCR Assays

Methods, 2001
The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra.
C T, Wittwer, M G, Herrmann, C N, Gundry, K S, Elenitoba-Johnson   +3 more
openaire   +2 more sources

The evolution of real-time PCR machines to real-time PCR chips

Biosensors and Bioelectronics, 2010
Development of Micro-Electro-Mechanical Systems (MEMS) technology has recently allowed the migration of real-time polymerase chain reaction (PCR) machines to lab-on-a-chip systems. The miniaturization of biological instruments has been studied extensively, with several prototypes constructed and tested.
Dasheng, Lee, Pei-Jer, Chen, Gwo-Bin, Lee   +2 more
openaire   +2 more sources

Real-Time PCR

2009
Over the past few years there has been a considerable development of DNA amplification by polymerase chain reaction (PCR), and real-time PCR has now superseded conventional PCR techniques in many areas, e.g., the quantification of nucleic acids and genotyping.
A. Evrard, N. Boulle, G.s Lutfalla
openaire   +1 more source

Real-Time PCR

Cold Spring Harbor Protocols, 2006
Wolfgang, Kusser, Sandrine, Javorschi, Martin A, Gleeson   +2 more
  +6 more sources

High-Throughput Real-Time PCR

2008
Real-time PCR is presently the gold standard of gene expression quantification. Configuration of real-time PCR instruments with 384-well reaction blocks, enables the instrument to be used essentially as a low-density array. While PCR will never rival the throughput of microchip arrays, in situations where one is interested in assaying several hundreds ...
Thomas D, Schmittgen, Eun Joo, Lee, Jinmai, Jiang   +2 more
openaire   +2 more sources

Real-Time PCR Fluorescent Chemistries

2007
There are more than a dozen formats available for the fluorescent detection of amplified DNA in kinetic (real-time) PCR. These chemistries are adaptable to most real-time PCR instruments and may offer benefits over the usual manufacturer-recommended chemistries for the instrument.
John, Mackay, Olfert, Landt
openaire   +2 more sources

Multiplex Real-Time PCR (MRT-PCR) for Diarrheagenic

2012
Diarrheagenic Escherichia coli strains are important causes of diarrhea in children from the developing world and are now being recognized as emerging enteropathogens in the developed world. Current methods of detection are too expensive and labor-intensive for routine detection of these organisms to be practical.
Barletta, Francesca, Ochoa Woodell, Theresa Jean, Cleary, Thomas G.   +2 more
openaire   +3 more sources

Real-time PCR.

2016
M. Ravnikar, N. Mehle, K. Gruden, T. Dreo   +3 more
  +4 more sources

An overview of real‐world data sources for oncology and considerations for research

Ca-A Cancer Journal for Clinicians, 2022
Lynne Penberthy, Donna R Rivera, Jennifer L Lund, Msph   +2 more
exaly