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Evaluation of a sensitive real-time PCR assay for Group B Streptococcus detection in vaginal-rectal swab. [PDF]
Sci RepWang X +5 more
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Novel dual-gene target and probe-based real-time PCR for the detection and differentiation of <i>Salmonella</i> Dublin. [PDF]
Appl Environ MicrobiolArevalo-Mayorga A +4 more
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Real-time PCR to target <i>Hoodia</i> in herbal supplements: a tool for conservation and trade regulation. [PDF]
Food Chem (Oxf)Fraiture MA +5 more
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The evolution of real-time PCR machines to real-time PCR chips
Biosensors and Bioelectronics, 2010Development of Micro-Electro-Mechanical Systems (MEMS) technology has recently allowed the migration of real-time polymerase chain reaction (PCR) machines to lab-on-a-chip systems. The miniaturization of biological instruments has been studied extensively, with several prototypes constructed and tested.
G B Lee, Pei-Jer Chen, Dasheng Lee
exaly +3 more sources
Real-time PCR detection chemistry
Clinica Chimica Acta, 2015Real-time PCR is the method of choice in many laboratories for diagnostic and food applications. This technology merges the polymerase chain reaction chemistry with the use of fluorescent reporter molecules in order to monitor the production of amplification products during each cycle of the PCR reaction.
E, Navarro +3 more
exaly +3 more sources
Enfermedades infecciosas y microbiologia clinica, 2004
The development of instruments that allow real-time monitoring of fluorescence within PCR reaction vessels is a significant advance in clinical bacteriology. The technology is very flexible, and many alternative instruments and fluorescent probe systems are currently available.
Slade, Neda, Kapitanović, Sanja
+8 more sources
The development of instruments that allow real-time monitoring of fluorescence within PCR reaction vessels is a significant advance in clinical bacteriology. The technology is very flexible, and many alternative instruments and fluorescent probe systems are currently available.
Slade, Neda, Kapitanović, Sanja
+8 more sources
Forensic Science, Medicine, and Pathology, 2007
Research into the field of DNA has increased dramatically in the years since DNA profiling was first identified. Since then, techniques have been developed to identify individuals from DNA samples equivalent to a single cell. Development of these techniques meant that the ability to accurately quantitate the DNA in such samples was required to evolve ...
+6 more sources
Research into the field of DNA has increased dramatically in the years since DNA profiling was first identified. Since then, techniques have been developed to identify individuals from DNA samples equivalent to a single cell. Development of these techniques meant that the ability to accurately quantitate the DNA in such samples was required to evolve ...
+6 more sources
2015
Wie bereits unter Kap. 13 erwahnt, ist die Quantifizierung der amplifizierten PCR-Produkte nach 25–30 Zyklen nicht mehr sehr einfach. Damit eine Quantifizierung der zu untersuchenden Amplicons (ca. 50–150 bp Lange) ermoglicht werden kann, haben sich verschiedene optische PCR-Systeme zur „online“ Beobachtung des Amplifikationsstatus etabliert (Tab. 14.1)
Hans-Joachim Müller +1 more
openaire +2 more sources
Wie bereits unter Kap. 13 erwahnt, ist die Quantifizierung der amplifizierten PCR-Produkte nach 25–30 Zyklen nicht mehr sehr einfach. Damit eine Quantifizierung der zu untersuchenden Amplicons (ca. 50–150 bp Lange) ermoglicht werden kann, haben sich verschiedene optische PCR-Systeme zur „online“ Beobachtung des Amplifikationsstatus etabliert (Tab. 14.1)
Hans-Joachim Müller +1 more
openaire +2 more sources
2009
Over the past few years there has been a considerable development of DNA amplification by polymerase chain reaction (PCR), and real-time PCR has now superseded conventional PCR techniques in many areas, e.g., the quantification of nucleic acids and genotyping.
A. Evrard, N. Boulle, G.s Lutfalla
openaire +1 more source
Over the past few years there has been a considerable development of DNA amplification by polymerase chain reaction (PCR), and real-time PCR has now superseded conventional PCR techniques in many areas, e.g., the quantification of nucleic acids and genotyping.
A. Evrard, N. Boulle, G.s Lutfalla
openaire +1 more source