Results 111 to 120 of about 490,106 (140)
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Electrochemical Real-Time Polymerase Chain Reaction
Journal of the American Chemical Society, 2006In this work, we report the first electrochemistry-based real-time polymerase chain reaction technique for sequence-specific nucleic acid detection. This new technique builds upon the advantages of the well-established fluorescence-based counterpart, such as short assay time (simultaneous target DNA amplification and detection).
Yeung, Siu Wai +2 more
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Real-Time Polymerase Chain Reaction Detection Methods
Recent Patents on DNA & Gene Sequences, 2008Real-time Polymerase Chain Reaction (PCR) is a quickly developing technology that has built upon the classic end-point PCR detection methods. In this article, we will review recent patents related to various chemistries used for nucleic acid detection during real-time PCR amplification.
Douglas F, Whitman, Sherry A, Dunbar
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Detection of Castor Contamination by Real-Time Polymerase Chain Reaction
Journal of Agricultural and Food Chemistry, 2006Due to the potential for intentional contamination of food with crude preparations containing ricin, a real-time PCR method was developed for the detection of castor plant material in ground beef. One primer pair was identified and confirmed to be castor-specific and efficient for amplification of ricin in DNA extracts from castor or beef matrices.
Xiaohua, He +4 more
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Robust regression methods for real-time polymerase chain reaction
Analytical Biochemistry, 2015Current real-time polymerase chain reaction (PCR) data analysis methods implement linear least squares regression methods for primer efficiency estimation based on standard curve dilution series. This method is sensitive to outliers that distort the outcome and are often ignored or removed by the end user.
Trypsteen, Wim +6 more
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The Use of Real-Time Polymerase Chain Reaction in Thoracic Malignancies
Thoracic Surgery Clinics, 2006Recent progress in the molecular analysis of NSCLC tumors and lymph node status will likely translate into a clearer understanding of the variables and predictors of tumor recurrence. This understanding may lead to more appropriate therapeutic decisions both in the operating room and in the clinic.
Jonathan, D'Cunha, Michael A, Maddaus
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Quantification of DNA by Real-Time Polymerase Chain Reaction (PCR)
Cold Spring Harbor Protocols, 2018There are few differences between the experimental steps necessary for amplifying template DNA in a real-time thermocycler and a standard polymerase chain reaction (PCR). In real-time PCR, it is necessary, however, to optimize the concentration of primers and probe and to perform a standard curve.
Michael R, Green, Joseph, Sambrook
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Detection of Plasmodium knowlesi by Real-Time Polymerase Chain Reaction
The American Journal of Tropical Medicine and Hygiene, 2009We previously developed a real-time polymerase chain reaction (PCR) assay for detection of the four Plasmodium species that infect humans. Recent studies have shown that natural transmission of the simian parasite Plasmodium knowlesi to humans occurs frequently in Southeast Asia. We have expanded our PCR assay to include detection of P. knowlesi.
N Esther, Babady +3 more
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Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction
2010The real-time quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) has become the method of choice for the quantification of specific mRNAs. This method is fast, extremely sensitive, and accurate, requires only very small amounts of input RNA, and is relatively simple to perform.
Hongxin, Fan, Ryan S, Robetorye
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Real-Time Polymerase Chain Reaction and Melting Curve Analysis
2006Monitoring polymerase chain reaction (PCR) once each cycle is a powerful method to detect and quantify the presence of nucleic acid sequences and has become known as "real-time" PCR. Absolute quantification of initial template copy number can be obtained, although quantification relative to a control sample or second sequence is often adequate. Melting
Robert J, Pryor, Carl T, Wittwer
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