Results 211 to 220 of about 731,111 (296)

Comparison of Real-time Polymerase Chain Reaction and Culture for Targeting Pathogens in Pediatric Severe Community-Acquired Pneumonia. [PDF]

Turk Arch Pediatr
Quang Tran K, Hung Pham V, Minh Vo C, Minh Pham Q, Minh Nguyen P.   +4 more
europepmc   +1 more source

A Rational Optimization Approach for the Development of a Multiplexed Lateral Flow Immunoassay: Detection of Nonepithelial Ovarian Cancer Markers in Human Serum

Advanced Science, EarlyView.
This study outlines the developmental pipeline of a multiplexed nanozyme‐based lateral flow immunoassay for the purpose of ovarian germ cell tumor detection. It demonstrates the application of a design of experiments optimization approach for nanozyme probe conjugate development.
Aida Abdelwahed, Carl E. Eskildsen, Luca Panariello, André Shamsabadi, Christy J. Sadler, Edmund H. Wilkes, Federico Galvanin, Yuxi Cheng, Sara Carvalho, Srdjan Saso, Molly M. Stevens   +10 more
wiley   +1 more source

Performance of real-time polymerase chain reaction and Kato-Katz for diagnosing soil-transmitted helminth infections and evaluating treatment efficacy of emodepside in randomized controlled trials. [PDF]

PLoS Negl Trop Dis
Lotz CN, Mrimi EC, Schneeberger PHH, Ali SM, Hattendorf J, Keiser J.   +5 more
europepmc   +1 more source

A Single‐Enzyme Activated CRISPR‐Cas12a Nano System via Subtly Balanced dsDNA for Kinetic‐Gated UDG Detection and Spatiotemporal Cellular Imaging

Advanced Science, EarlyView.
Kinetic‐gated CRISPR–Cas12a activation enables single‐enzyme detection and spatiotemporal imaging of UDG ABSTRACT Uracil‐DNA glycosylase (UDG) is a key enzyme in base excision repair and an important biomarker for genomic stability and disease. In many reported sensing systems, uracil excision is coupled to signal generation through additional ...
Kejun Dong, Hao Hu, Haiyun Wang, Zijia Zheng, Shuangshuang Cheng, Wan Shu, Ruisi Liu, Xiaoyan Xin, Shiyi Huang, Dingchao Qian, Xianjin Xiao, Qiang Fu, Hongbo Wang   +12 more
wiley   +1 more source

TaqMan-based real-time polymerase chain reaction for the detection of feline chaphamaparvovirus. [PDF]

3 Biotech
Li S, Huo X, Mu Y, Liu X, Wu J, Chen Y, Wang Y.   +6 more
europepmc   +1 more source

Design of Optical Transducer for Recognition of Biomolecular Interactions between Bacterial Lipopolysaccharides and Amino Acids

Advanced Science, EarlyView.
A deep comprehension of biomolecular phenomena at interfaces is significant with their fundamental and practical importance. Our experimental and theoretical investigations reveal that specific amino acids (glutamic acid and aspartic acid) exhibit an orientational coupling with liquid crystals, which can recognize and optically report the interfacial ...
Yena Choi, Sangmin Lee, Jin‐Kang Choi, Hyunsoo Han, Yeongseon Choi, Jun‐Hyung Im, Hyein Kim, Sangmin Jeon, Minjae Lee, Chang Yun Son, Young‐Ki Kim   +10 more
wiley   +1 more source

Melting curve analyses in the quantitative real-time polymerase chain reaction of methylated/non-methylated DNA toward the detection of oral cancer using gargle fluid. [PDF]

Heliyon
Noma Y, Sawayama H, Yamashiro K, Nishi K, Yoshinaga H, Takaoka R, Sugiura T, Sasahira T, Hamada T, Suda Y, Okui T.   +10 more
europepmc   +1 more source

Multi‐Omics Insights Into the Mechanisms of Early Muscle Fiber Difference and Transformation Between Lean‐Type and Chinese Indigenous Pigs

Advanced Science, EarlyView.
Multi‐omics analyses uncover breed‐specific cis‐regulatory landscapes and higher‐order chromatin architectural differences that underlie early postnatal muscle fiber divergence in pigs. A super‐enhancer upstream of PPP3CB recruits MEF2C to activate PPP3CB transcription, while the PPP3CB–MEF2C positive feedback loop promotes oxidative muscle fiber ...
Shuailong Zheng, Hainan Wu, Min Liu, Kunpeng Wu, Shuntao Huang, Zulfiqar Ahmed, Lenox Pius, Mengjin Zhu, Dequan Xu   +8 more
wiley   +1 more source

Comparative Analysis of Gene Expression at Sea Level and High Altitude: A Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) Approach. [PDF]

Cureus
Alharthi SB, Alsubai AH, Almalki SK, El-Shehawi AM, Eldebsy AM, Alsoliman AA, Alharthi RF, Morsi M, Alharthi D, Mutabaqani RA.   +9 more
europepmc   +1 more source

CRISPR/Cas13a: Compensatory Target Activation Mechanism

Advanced Science, EarlyView.
CRISPR/Cas13a‐CTAM as a novel compensatory target activation mechanism that enables synergistic activation of Cas13a via two independently editable short RNA effectors. This dual‐effector system maintains enzymatic activity comparable to traditional single‐effector while significantly enhancing flexibility, sensitivity, and application scope.
Bowen Jiang, Tenghua Zhang, Yao Lu, Shujun Zhou, Wenjing Xiao, Shuming Pan, Nan Shi, Yan Sheng, Jiaming Hu   +8 more
wiley   +1 more source