Results 251 to 260 of about 644,089 (298)
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Journal of Biochemical and Molecular Toxicology, 2004
AbstractAn efficient and effective method for quantification of small amounts of nucleic acids contained within a sample specimen would be an important diagnostic tool for determining the content of mitochondrial DNA (mtDNA) in situations where the depletion thereof may be a contributing factor to the exhibited pathology phenotype.
Hari K, Bhat, Irina, Epelboym
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AbstractAn efficient and effective method for quantification of small amounts of nucleic acids contained within a sample specimen would be an important diagnostic tool for determining the content of mitochondrial DNA (mtDNA) in situations where the depletion thereof may be a contributing factor to the exhibited pathology phenotype.
Hari K, Bhat, Irina, Epelboym
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Real-Time Polymerase Chain Reaction and Melting Curve Analysis
2006Monitoring polymerase chain reaction (PCR) once each cycle is a powerful method to detect and quantify the presence of nucleic acid sequences and has become known as "real-time" PCR. Absolute quantification of initial template copy number can be obtained, although quantification relative to a control sample or second sequence is often adequate. Melting
Robert J, Pryor, Carl T, Wittwer
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Detection of Castor Contamination by Real-Time Polymerase Chain Reaction
Journal of Agricultural and Food Chemistry, 2006Due to the potential for intentional contamination of food with crude preparations containing ricin, a real-time PCR method was developed for the detection of castor plant material in ground beef. One primer pair was identified and confirmed to be castor-specific and efficient for amplification of ricin in DNA extracts from castor or beef matrices.
Xiaohua, He +4 more
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Real-Time Quantitative Polymerase Chain Reaction to Assess Gene Transfer
Human Gene Therapy, 1999Accurate quantification of gene transfer (or gene correction) is a universal challenge in the field of gene therapy. In developing a clinical trial of lymphocyte gene therapy for Hunter syndrome (mucopolysaccharidosis type II), methods using Southern blot or automated DNA sequencing technology were employed, but found to be laborious and subject to ...
K, Becker, D, Pan, C B, Whitley
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Quantification of DNA by Real-Time Polymerase Chain Reaction (PCR)
Cold Spring Harbor Protocols, 2018There are few differences between the experimental steps necessary for amplifying template DNA in a real-time thermocycler and a standard polymerase chain reaction (PCR). In real-time PCR, it is necessary, however, to optimize the concentration of primers and probe and to perform a standard curve.
Michael R, Green, Joseph, Sambrook
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[Real-time polymerase chain reaction (RT-PCR)].
Annales Academiae Medicae Stetinensis, 2008The work present PCR (Polymerase Chain Reaction) with the real time analysis of the increase of product allowing quick detection, duplication and identification of even slight amounts of nucleic acids. Essential methods used in molecular diagnostics, based on marked probes and fluorescence phenomenon were discussed. Increased sensitivity of technology,
Katarzyna, Wiedro +2 more
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Real-time polymerase chain reaction
Resonance, 2008Simarjot Singh Pabla +1 more
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Polymerase Chain Reaction (PCR) and Real-Time PCR
2013The PCR technique, developed over 3 decades ago, has revolutionized molecular biology. PCR applications encompass several fields, including genetics, molecular cloning, diagnosis of infectious and hereditary diseases, and forensic sciences. Improvements of the PCR technique have resulted in its expansion to include variants such as reverse ...
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