Multiplex Real-Time Polymerase Chain Reaction Assay To Detect Acanthamoeba spp., Vermamoeba vermiformis, Naegleria fowleri, and Balamuthia mandrillaris in Different Water Sources. [PDF]
Córdoba-Lanús E +10 more
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Duplex Reverse-Transcription Real-Time Polymerase Chain Reaction Assay Targeting 23S rRNA Single Nucleotide Polymorphisms for the Detection of Flea-Borne Rickettsioses. [PDF]
Probert WS +3 more
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Seasonal Stability Assessment of Reference Genes for Quantitative Real-Time Polymerase Chain Reaction Normalization in Bombus terrestris. [PDF]
Sankar K +4 more
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Validation of a real-time polymerase chain reaction for the detection and quantification of the nucleic acid of Histoplasma from equine clinical samples. [PDF]
Fisher LWS +8 more
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Revolutionizing chronic endometritis diagnosis: real-time polymerase chain reaction unveils microbial pathogens in Indian women with abnormal bleeding and reproductive challenges. [PDF]
Nandagopal M +5 more
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The real-time polymerase chain reaction
Molecular Aspects of Medicine, 2006The scientific, medical, and diagnostic communities have been presented the most powerful tool for quantitative nucleic acids analysis: real-time PCR [Bustin, S.A., 2004. A-Z of Quantitative PCR. IUL Press, San Diego, CA]. This new technique is a refinement of the original Polymerase Chain Reaction (PCR) developed by Kary Mullis and coworkers in the ...
Mikael, Kubista +11 more
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Real‐Time Polymerase Chain Reaction
ChemBioChem, 2003AbstractReal‐time PCR is the state‐of‐the‐art technique to quantify nucleic acids for mutation detection, genotyping and chimerism analysis. Since its development in the 1990s, many different assay formats have been developed and the number of real‐time PCR machines of different design is continuously increasing.
Jochen, Wilhelm, Alfred, Pingoud
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Electrochemical Real-Time Polymerase Chain Reaction
Journal of the American Chemical Society, 2006In this work, we report the first electrochemistry-based real-time polymerase chain reaction technique for sequence-specific nucleic acid detection. This new technique builds upon the advantages of the well-established fluorescence-based counterpart, such as short assay time (simultaneous target DNA amplification and detection).
Yeung, Siu Wai +2 more
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Real-Time Polymerase Chain Reaction Detection Methods
Recent Patents on DNA & Gene Sequences, 2008Real-time Polymerase Chain Reaction (PCR) is a quickly developing technology that has built upon the classic end-point PCR detection methods. In this article, we will review recent patents related to various chemistries used for nucleic acid detection during real-time PCR amplification.
Douglas F, Whitman, Sherry A, Dunbar
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