Results 241 to 250 of about 731,111 (296)

Multiplex Real-Time Polymerase Chain Reaction Assay To Detect Acanthamoeba spp., Vermamoeba vermiformis, Naegleria fowleri, and Balamuthia mandrillaris in Different Water Sources. [PDF]

open access: yesAm J Trop Med Hyg
Córdoba-Lanús E   +10 more
europepmc   +1 more source

Validation of a real-time polymerase chain reaction for the detection and quantification of the nucleic acid of Histoplasma from equine clinical samples. [PDF]

open access: yesMicrobiol Spectr
Fisher LWS   +8 more
europepmc   +1 more source

The real-time polymerase chain reaction

Molecular Aspects of Medicine, 2006
The scientific, medical, and diagnostic communities have been presented the most powerful tool for quantitative nucleic acids analysis: real-time PCR [Bustin, S.A., 2004. A-Z of Quantitative PCR. IUL Press, San Diego, CA]. This new technique is a refinement of the original Polymerase Chain Reaction (PCR) developed by Kary Mullis and coworkers in the ...
Mikael, Kubista   +11 more
openaire   +4 more sources

Real‐Time Polymerase Chain Reaction

ChemBioChem, 2003
AbstractReal‐time PCR is the state‐of‐the‐art technique to quantify nucleic acids for mutation detection, genotyping and chimerism analysis. Since its development in the 1990s, many different assay formats have been developed and the number of real‐time PCR machines of different design is continuously increasing.
Jochen, Wilhelm, Alfred, Pingoud
openaire   +2 more sources

Electrochemical Real-Time Polymerase Chain Reaction

Journal of the American Chemical Society, 2006
In this work, we report the first electrochemistry-based real-time polymerase chain reaction technique for sequence-specific nucleic acid detection. This new technique builds upon the advantages of the well-established fluorescence-based counterpart, such as short assay time (simultaneous target DNA amplification and detection).
Yeung, Siu Wai   +2 more
openaire   +3 more sources

Real-Time Polymerase Chain Reaction Detection Methods

Recent Patents on DNA & Gene Sequences, 2008
Real-time Polymerase Chain Reaction (PCR) is a quickly developing technology that has built upon the classic end-point PCR detection methods. In this article, we will review recent patents related to various chemistries used for nucleic acid detection during real-time PCR amplification.
Douglas F, Whitman, Sherry A, Dunbar
openaire   +2 more sources

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