Results 251 to 260 of about 731,111 (296)

Robust regression methods for real-time polymerase chain reaction

Analytical Biochemistry, 2015
Current real-time polymerase chain reaction (PCR) data analysis methods implement linear least squares regression methods for primer efficiency estimation based on standard curve dilution series. This method is sensitive to outliers that distort the outcome and are often ignored or removed by the end user.
Trypsteen, Wim, De Neve, Jan, Bosman, Kobus, Nijhuis, Monique, Thas, Olivier, Vandekerckhove, Linos, De Spiegelaere, Ward   +6 more
openaire   +4 more sources

Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction

2010
The real-time quantitative reverse transcriptase polymerase chain reaction (RQ-PCR) has become the method of choice for the quantification of specific mRNAs. This method is fast, extremely sensitive, and accurate, requires only very small amounts of input RNA, and is relatively simple to perform.
Hongxin, Fan, Ryan S, Robetorye
openaire   +2 more sources

Quantitative analysis of total mitochondrial DNA: Competitive polymerase chain reaction versus real‐time polymerase chain reaction

Journal of Biochemical and Molecular Toxicology, 2004
AbstractAn efficient and effective method for quantification of small amounts of nucleic acids contained within a sample specimen would be an important diagnostic tool for determining the content of mitochondrial DNA (mtDNA) in situations where the depletion thereof may be a contributing factor to the exhibited pathology phenotype.
Hari K, Bhat, Irina, Epelboym
openaire   +2 more sources

Real-Time Polymerase Chain Reaction and Melting Curve Analysis

2006
Monitoring polymerase chain reaction (PCR) once each cycle is a powerful method to detect and quantify the presence of nucleic acid sequences and has become known as "real-time" PCR. Absolute quantification of initial template copy number can be obtained, although quantification relative to a control sample or second sequence is often adequate. Melting
Robert J, Pryor, Carl T, Wittwer
openaire   +2 more sources

Detection of Castor Contamination by Real-Time Polymerase Chain Reaction

Journal of Agricultural and Food Chemistry, 2006
Due to the potential for intentional contamination of food with crude preparations containing ricin, a real-time PCR method was developed for the detection of castor plant material in ground beef. One primer pair was identified and confirmed to be castor-specific and efficient for amplification of ricin in DNA extracts from castor or beef matrices.
Xiaohua, He, David L, Brandon, Grace Q, Chen, Thomas A, McKeon, John Mark, Carter   +4 more
openaire   +2 more sources

Real-Time Quantitative Polymerase Chain Reaction to Assess Gene Transfer

Human Gene Therapy, 1999
Accurate quantification of gene transfer (or gene correction) is a universal challenge in the field of gene therapy. In developing a clinical trial of lymphocyte gene therapy for Hunter syndrome (mucopolysaccharidosis type II), methods using Southern blot or automated DNA sequencing technology were employed, but found to be laborious and subject to ...
K, Becker, D, Pan, C B, Whitley
openaire   +2 more sources

Quantification of DNA by Real-Time Polymerase Chain Reaction (PCR)

Cold Spring Harbor Protocols, 2018
There are few differences between the experimental steps necessary for amplifying template DNA in a real-time thermocycler and a standard polymerase chain reaction (PCR). In real-time PCR, it is necessary, however, to optimize the concentration of primers and probe and to perform a standard curve.
Michael R, Green, Joseph, Sambrook
openaire   +2 more sources

[Real-time polymerase chain reaction (RT-PCR)].

Annales Academiae Medicae Stetinensis, 2008
The work present PCR (Polymerase Chain Reaction) with the real time analysis of the increase of product allowing quick detection, duplication and identification of even slight amounts of nucleic acids. Essential methods used in molecular diagnostics, based on marked probes and fluorescence phenomenon were discussed. Increased sensitivity of technology,
Katarzyna, Wiedro, Ewa, Stachowska, Dariusz, Chlubek   +2 more
openaire   +1 more source

Real-time polymerase chain reaction

2023
Javeed Ahmad Tantray, Sheikh Mansoor, Rasy Fayaz Choh Wani, Nighat Un Nissa   +3 more
openaire   +1 more source

Real-Time Polymerase Chain Reaction

2006
Heng-Phon Too, Azlinda Anwar
openaire   +2 more sources