Results 191 to 200 of about 21,194,347 (300)

Spatial Control of Light‐Responsive Proteins and Optogenetics Within Hydrogels via Volumetric Bioprinting

Advanced Materials, EarlyView.
Spatiotemporal control over cell behavior within bioprinted constructs remains a key challenge in biofabrication. Volumetric bioprinting using multi‐wavelength light sources permits to activate light‐sensitive intracellular proteins as well as optogenetic gene and protein expression circuits in bioprinted cells, in addition to enabling fast printing of
Davide Ribezzi, Pere Català, Gabriel Größbacher, Paulina Nuñez Bernal, Olaf Nijssen, Sammy Florczak, Wilco Nijenhuis, Nuria Crusellas‐Villorbina, Bram Nijhoff, Paul Delrot, Jos Malda, Andreas Hierholzer, Martin Fussenegger, Lukas C. Kapitein, Riccardo Levato   +14 more
wiley   +1 more source

Culture and real-time quantitative PCR to detect environmental nontuberculous mycobacteria in a clinical care center. [PDF]

Total Environ Microbiol
Zhang M, Smith BL, Wilsey RN, Koch TNM, Dohanich E, McShane PJ, Honda JR, King MD.   +7 more
europepmc   +1 more source

Rare Variants in RPPH1 Real-Time Quantitative PCR Control Assay Binding Sites Result in Incorrect Copy Number Calls. [PDF]

J Mol Diagn, 2022
Sicko RJ, Romitti PA, Browne ML, Brody LC, Stevens CF, Mills JL, Caggana M, Kay DM.   +7 more
europepmc   +1 more source

Accessing Planktothrix species diversity and associated toxins using quantitative real-time PCR in natural waters

, 2015
Tese de Doutoramento em Biologia apresentada à Faculdade de Ciências da Universidade do Porto, 2015.I acknowledge all the institutions and laboratories that have received me and/or provided logistic support and equipment to conduct the experiments.
Churro, Catarina
core  

Electropolymerized Aptasensor for Femtogram‐Level Cytokine Detection

Advanced Materials, EarlyView.
Electropolymerization of 3‐amino‐ʟ‐tyrosine creates ultrathin, smooth, and chemically robust pALT nanofilms with accessible carboxyl functionalities for direct aptamer immobilization. Integrated with fiber‐optic SPR and OECT platforms, these interfaces enable IL‐6 detection down to 100 fg mL−1 and provide a versatile route to bioelectronic sensing ...
Jiyao Yu, Renan Colucci, Rachel X. Shi, Qi Lu, Tommaso Marchesi D'Alvise, Ulrike Kraft, Christopher V. Synatschke, Tanja Weil   +7 more
wiley   +1 more source

Development of a Real-Time Quantitative PCR Assay for Direct Detection and Quantification of the Root-Lesion Nematode <i>Pratylenchus penetrans</i> in Potato Roots. [PDF]

Int J Mol Sci
Poudel D, Yan G.
europepmc   +1 more source

Detection and relative quantification of amine oxidase gene (yobN) in Bacillus subtilis: application of real-time quantitative PCR. [PDF]

J Food Sci Technol, 2022
Pištěková H, Jančová P, Buňková L, Šopík T, Maršálková K, Berčíková L, Buňka F.   +6 more
europepmc   +1 more source

Esterified Cholesterol Conjugates Enable LNP‐Mediated mRNA Delivery to the Blood–Brain Barrier Following Intravenous Administration

Advanced Materials, EarlyView.
A cholesterol‐conjugated lipid library enabled the identification of a ligand‐free LNP platform for efficient mRNA delivery to brain endothelial cells via systemic administration. This platform achieves selective BBB targeting without disrupting barrier integrity, and enables modulation of neuroinflammation and vascular function without requiring trans‐
Zeru Tian, Xu Wang, Alexis Pacheco Benitez, Erick Guerrero, Yaou Duan, Jingwen Wei, Lukas Farbiak, Daniel J. Siegwart   +7 more
wiley   +1 more source

Selection and validation of stable reference genes in potato infected by Pectobacterium atrosepticum using real-time quantitative PCR. [PDF]

Sci Rep
Hou L, Zhu H, Xian W, Ma Y.
europepmc   +1 more source

Comparison and Sensitivity Evaluation of Three Different Commercial Real-Time Quantitative PCR Kits for SARS-CoV-2 Detection. [PDF]

Viruses, 2021
Banko A, Petrovic G, Miljanovic D, Loncar A, Vukcevic M, Despot D, Cirkovic A.   +6 more
europepmc   +1 more source