Results 71 to 80 of about 993,329 (331)

Modeling hepatic fibrosis in TP53 knockout iPSC‐derived human liver organoids

Molecular Oncology, EarlyView.
This study developed iPSC‐derived human liver organoids with TP53 gene knockout to model human liver fibrosis. These organoids showed elevated myofibroblast activation, early disease markers, and advanced fibrotic hallmarks. The use of profibrotic differentiation medium further amplified the fibrotic signature seen in the organoids.
Mustafa Karabicici, Soheil Akbari, Ceyda Caliskan, Canan Celiker, Ozden Oz, Leman Binokay, Gökhan Karakulah, Serif Senturk, Esra Erdal   +8 more
wiley   +1 more source

Application of droplet digital PCR for quantitative detection of Spiroplasma citri in comparison with real time PCR.

PLoS ONE, 2017
Droplet digital polymerase chain reaction (ddPCR) is a method for performing digital PCR that is based on water-oil emulsion droplet technology. It is a unique approach to measure the absolute copy number of nucleic acid targets without the need of ...
Yogita Maheshwari, Vijayanandraj Selvaraj, Subhas Hajeri, Raymond Yokomi   +3 more
doaj   +1 more source

Standard and Light-Cycler PCR methods for animal DNA species detection in animal feedstuffs [PDF]

, 2008
In this work four species-specific primers and probes were designed and evaluated for the detection and quantification of bovine, ovine, swine and chicken mitochondrial DNA in feeds.
Ajmone_Marsan, P, Chegdani, F, Chiappini, B, Faggionato, E, Fontana, C, Frezza, D, Giambra, V, Lanni, L, Losio, N, Maccabiani, G, Saccares, S, Vaccari, G, von Holst, C   +12 more
core   +1 more source

PYCR1 inhibition in bone marrow stromal cells enhances bortezomib sensitivity in multiple myeloma cells by altering their metabolism

Molecular Oncology, EarlyView.
This study investigated how PYCR1 inhibition in bone marrow stromal cells (BMSCs) indirectly affects multiple myeloma (MM) cell metabolism and viability. Culturing MM cells in conditioned medium from PYCR1‐silenced BMSCs impaired oxidative phosphorylation and increased sensitivity to bortezomib.
Inge Oudaert, Lauren van den Broecke, Osman Aksoy, Judith Lind, Sonia Vallet, Arne Van der Vreken, Gamze Ates, Ann Massie, Ken Maes, Kim De Veirman, Elke De Bruyne, Karin Vanderkerken, Klaus Podar, Eline Menu   +13 more
wiley   +1 more source

Soil Baiting, Rapid PCR Assay and Quantitative Real TIME PCR to Diagnose Late Blight of Potato in Quarantine Programs [PDF]

, 2018
Phytophthora infestans (mont) de Bary is a pathogen of great concern across the globe, and accurate detection is an important component in responding to the outbreaks of potential disease.
Anwar, F. (Firoz), Hussain, T. (Touseef), Singh, B. P. (Bir)   +2 more
core   +2 more sources

Adaptaquin is selectively toxic to glioma stem cells through disruption of iron and cholesterol metabolism

Molecular Oncology, EarlyView.
Adaptaquin selectively kills glioma stem cells while sparing differentiated brain cells. Transcriptomic and proteomic analyses show Adaptaquin disrupts iron and cholesterol homeostasis, with iron chelation amplifying cytotoxicity via cholesterol depletion, mitochondrial dysfunction, and elevated reactive oxygen species.
Adrien M. Vaquié, Davod R. Shah, Eliane E. S. Brechbühl, Michael McNicholas, Zhaoyang Xu, John H. Stockley, Laura Morcom, Diana Gold Diaz, Gemma C. Girdler, Rachel V. Seear, Gabriel Balmus, Rajiv R. Ratan, Harry Bulstrode, James A. Nathan, Manav Pathania, Kevin M. Brindle, David H. Rowitch   +16 more
wiley   +1 more source

Nephrin and podocin mRNA detection in urine sediment of dogs with chronic kidney disease: preliminary observations

Journal of Veterinary Research, 2022
Dogs with chronic kidney disease (CKD) may have alterations in the glomerular filtration barrier, including podocyte loss. Detection of podocyte mRNA in urine could be useful for assessing podocyturia in dogs with kidney disease.
Souza Camilla de, Coelho Mariana, Antonelo Daniel Silva, Passarelli Danielle, Rochetti Arina Lázaro, Fukumasu Heidge, Leite-Dellova Deise Carla Almeida   +6 more
doaj   +1 more source

Aggressive prostate cancer is associated with pericyte dysfunction

Molecular Oncology, EarlyView.
Tumor‐produced TGF‐β drives pericyte dysfunction in prostate cancer. This dysfunction is characterized by downregulation of some canonical pericyte markers (i.e., DES, CSPG4, and ACTA2) while maintaining the expression of others (i.e., PDGFRB, NOTCH3, and RGS5).
Anabel Martinez‐Romero, Ane Martinez‐Larrinaga, Joaquim Grego‐Bessa, Saioa Garcia‐Longarte, Hielke van Splunder, Ianire Astobiza, Amaia Ercilla, Laura Bozal‐Basterra, Isabel Mendizabal, Pilar Villacampa, Arkaitz Carracedo, Mariona Graupera   +11 more
wiley   +1 more source

Quantification of Intestinal Lactobacillus Species in Children with Functional Constipation by Quantitative Real-Time PCR

Clinical and Experimental Gastroenterology, 2020
Nabi Jomehzadeh,1– 3 Hazhir Javaherizadeh,4 Mansour Amin,2,3 Mohammad Rashno,1 Ali Teimoori5 1Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; 2Infectious and Tropical Diseases Research Center ...
Jomehzadeh N, Javaherizadeh H, Amin M, Rashno M, Teimoori A   +4 more
doaj  

Selection and validation of reference genes for the normalization of quantitative real-time PCR in different muscle tissues of rabbits

BMC Zoology, 2022
Background In molecular biology studies, the selection of optimal reference genes is of vital importance for accurately quantifying gene expression.
Mengke Ni, Zhichao Li, Jing Li, Hui He, Yaling Wang, Yixuan Jiang, Xianwei Wang, Zhuanjian Li, Ming Li, Huifen Xu   +9 more
doaj   +1 more source