Results 71 to 80 of about 1,064,076 (204)

Ex vivo screening for immunodominant viral epitopes by quantitative real time polymerase chain reaction (qRT-PCR) [PDF]

, 2003
The identification and characterization of viral epitopes across the Human Leukocyte Antigen (HLA) polymorphism is critical for the development of actives-specific or adoptive immunotherapy of virally-mediated diseases.
Nagorsen Dirk, Bonginelli Paola, Mocellin Simone, Provenzano Maurizio, Kwon Seog-Woon, Stroncek David   +5 more
core   +2 more sources

Quantitative Detection of t(14;18)-Positive Cells by Real-Time Quantitative PCR Using Fluorogenic Probes

BioTechniques, 1998
To detect t(14;18)-positive cells present in human lymphoma tissue, bone marrow aspirates and peripheral blood mononuclear cells (PBMNC), we have established an automated, real-time quantitative PCR using double-labeled fluorogenic probes. In relation to
Dölken Lars, Schüler Frank, Dölken Gottfried   +2 more
doaj   +1 more source

Soil Baiting, Rapid PCR Assay and Quantitative Real TIME PCR to Diagnose Late Blight of Potato in Quarantine Programs [PDF]

, 2018
Phytophthora infestans (mont) de Bary is a pathogen of great concern across the globe, and accurate detection is an important component in responding to the outbreaks of potential disease.
Anwar, F. (Firoz), Hussain, T. (Touseef), Singh, B. P. (Bir)   +2 more
core   +2 more sources

Enhanced cardiac expression of two isoforms of matrix metalloproteinase-2 in experimental diabetes mellitus. [PDF]

, 2019
BackgroundDiabetic cardiomyopathy (DM CMP) is defined as cardiomyocyte damage and ventricular dysfunction directly associated with diabetes independent of concomitant coronary artery disease or hypertension.
Ahn, Jinhee, Bae, Sun Sik, Cha, Kwang Soo, Choi, Jung Hyun, Hong, Taek Jong, Kim, Sang Sik, Lee, Han Cheol, Lee, Hye Won, Lee, Min Young, Lee, Sun Ju, Lovett, David H, Oh, Jun-Hyok, Park, Jin-Sup, Park, Mi Wha, Shin, Nari, Song, Sang Heon   +15 more
core   +2 more sources

Standard and Light-Cycler PCR methods for animal DNA species detection in animal feedstuffs [PDF]

, 2008
In this work four species-specific primers and probes were designed and evaluated for the detection and quantification of bovine, ovine, swine and chicken mitochondrial DNA in feeds.
Ajmone_Marsan, P, Chegdani, F, Chiappini, B, Faggionato, E, Fontana, C, Frezza, D, Giambra, V, Lanni, L, Losio, N, Maccabiani, G, Saccares, S, Vaccari, G, von Holst, C   +12 more
core   +1 more source

[Real time quantitative PCR].

Experimental & molecular medicine, 2002
So far, quantitative techniques, such as PCR and FISH, have been used to detect of DNA and RNA. However, it is difficult to measure and compare the exact amount of amplified products with the results of endpoint analysis in conventional PCR techniques.
openaire   +1 more source

Quantitative real time PCR for distinction between Pneumocystis jirovecii infection/colonization in hospitalized patients

Frontiers in Cellular and Infection Microbiology
BackgroundIdentification of the opportunistic fungus Pneumocystis jirovecii in respiratory specimens presents challenges, particularly in differentiating between colonization and active infection.
Faezeh Rouhi, Mahzad Erami, Sepide Rastgufar, Maryam Jahani, Shima Aboutalebian, Shima Aboutalebian, Sajedeh Soltani, Hamed Fakhim, Hossein Mirhendi, Hossein Mirhendi   +9 more
doaj   +1 more source

A triplex quantitative real-time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7

Virology Journal, 2018
Background Human adenovirus (HAdV) serotypes 2, 3 and 7 are more prevalent than other serotypes and have been associated with severe pneumonia in pediatric children.
Fang-zhou Qiu, Xin-xin Shen, Meng-chuan Zhao, Li Zhao, Su-xia Duan, Chen Chen, Ju-Ju Qi, Gui-xia Li, Le Wang, Zhi-shan Feng, Xue-jun Ma   +10 more
doaj   +1 more source

自发性高血压大鼠血液miRNAs表达分析

Zhongguo shiyan zhenduanxue, 2014
目的本试验检测自发性高血压大鼠（SHR）血液中miR-448-5p、miR-128、miR-146a和miR-24-2*四种小分子RNA（miRNA）的表达,探讨高血压疾病与miRNA表达水平相关性,为人类高血压诊断及治疗提供新的靶标。方法应用荧光定量PCR方法对SHR大鼠和正常大鼠血液中miR-448-5p、miR-128、miR-146a和miR-24-2*表达量进行检测。结果与正常对照组相比,SHR大鼠血液中miR-448-5p、miR-128、miR-146a和miR-24-2 ...
鞠传静, 王旭渤, 梁斌斌, 李颖, 于冰   +4 more
doaj  

Comparison of digital PCR and real time PCR methods for quantitative analysis of African Swine Fever Virus

Frontiers in Veterinary Science
IntroductionThe African swine fever (ASF) is a viral disease of significant socio-economic and animal health impact, commonly diagnosed using molecular techniques.
Silvia Dei Giudici, Piero Bonelli, Maria Giovanna Tilocca, Simona Cau, Pier Paolo Angioi, Anna Maria Sechi, Bruna Vodret, Annalisa Oggiano   +7 more
doaj   +1 more source