Results 71 to 80 of about 22,071,005 (304)

Application of droplet digital PCR for quantitative detection of Spiroplasma citri in comparison with real time PCR.

open access: yesPLoS ONE, 2017
Droplet digital polymerase chain reaction (ddPCR) is a method for performing digital PCR that is based on water-oil emulsion droplet technology. It is a unique approach to measure the absolute copy number of nucleic acid targets without the need of ...
Yogita Maheshwari   +3 more
doaj   +1 more source

Quantitative real-time PCR v1

open access: yes, 2023
Quantitative real-time PCR protocol used for Day et al.
openaire   +1 more source

HBV DNA Quantitation Using Real-time PCR

open access: yesAnnals of Laboratory Medicine, 2006
Accurate measurement of the concentration of hepatitis B virus (HBV) DNA in clinical samples is important for the appropriate treatment of patients and evaluation of their therapeutic responses. In addition, the concentration of HBV DNA in the serum of patients with chronic HBV infection has a very broad range.
Jeong, Heo   +7 more
openaire   +2 more sources

Pharmacological chromatin remodeling enhances response to estrogen therapy in ER+ breast cancer

open access: yesMolecular Oncology, EarlyView.
Estrogen therapy elicits clinical benefit in ~ 30% of patients with endocrine‐resistant estrogen receptor (ER)‐positive breast cancer. Based on findings that ER transcriptional activation underlies response to estrogen therapy, we tested the effects of epigenetic dysregulation via pharmacological inhibition of histone deacetylases (HDACi).
Anneka L. Johnson Thomas   +16 more
wiley   +1 more source

Primers for quantitative real-time PCR.

open access: yes, 2016
Primers for quantitative real-time PCR.
Qing-song Lei (3168369)   +4 more
core   +2 more sources

Identification and validation of stable reference genes for quantitative real time PCR in different minipig tissues at developmental stages

open access: yesBMC Genomics, 2022
Background Quantitative real time PCR (qPCR) is a powerful tool to evaluate mRNA expression level. However, reliable qPCR results require normalization with validated reference gene(s).
Jeongah Song   +3 more
doaj   +1 more source

Somatostatin receptor 4 (SSTR4) is a tumor suppressor in cutaneous and head & neck squamous cell carcinomas

open access: yesMolecular Oncology, EarlyView.
This study identifies somatostatin receptor 4 (Sstr4) as a critical tumor suppressor against skin and head/neck cancers (HNSCC, cSCC, and BCC). The loss of Sstr4 removes a check on cell growth, causing hyperactivation of the MAPK‐ERK signaling pathway (↑).
Ali Taqvi   +6 more
wiley   +1 more source

Detection of Saccharopolyspora rectivirgula by quantitative Real-Time PCR

open access: yesThe Annals of occupational hygiene, 2011
The thermophilic actinomycete species Saccharopolyspora rectivirgula has been associated with the exogen allergic alveolitis (EAA). EAA is caused by the inhalation of high amounts of airborne spores that can be found for example in environments of agricultural production, compost facilities, mushroom cultivation rooms, or rooms with technical air ...
Schäfer, Jenny   +3 more
openaire   +4 more sources

Gut microbiota alterations in patients with non‐small‐cell lung cancer undergoing chemoradiotherapy

open access: yesMolecular Oncology, EarlyView.
We investigated the impact of concurrent chemoradiotherapy (CRT) on the gut microbiota in patients with locally advanced non‐small‐cell lung cancer. Overall gut microbiota composition remained largely stable throughout CRT while antibiotic exposure may influence microbial diversity.
Hanne Marte Nymoen   +19 more
wiley   +1 more source

Primers used for quantitative real-time PCR (qRT-PCR).

open access: yes, 2013
Primers used for quantitative real-time PCR (qRT-PCR).
Birgit Schittek (209307)   +4 more
core   +1 more source

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