Results 11 to 20 of about 3,905,031 (329)

Real-time PCR in virology [PDF]

Nucleic Acids Research, 2002
The use of the polymerase chain reaction (PCR) in molecular diagnostics has increased to the point where it is now accepted as the gold standard for detecting nucleic acids from a number of origins and it has become an essential tool in the research laboratory.
Mackay, I. M., Arden, K. E., Nitsche, A.
openaire   +4 more sources

Real-time PCR applications for diagnosis of leishmaniasis

Parasites & Vectors, 2018
Leishmaniasis is a vector-borne disease caused by many Leishmania species, which can infect both humans and other mammals. Leishmaniasis is a complex disease, with heterogeneous clinical manifestations ranging from asymptomatic infections to lesions at ...
Luca Galluzzi, Marcello Ceccarelli, Aurora Diotallevi, Michele Menotta, Mauro Magnani   +4 more
doaj   +2 more sources

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 2020
Background The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially ...
V. Corman, O. Landt, M. Kaiser, R. Molenkamp, A. Meijer, D. Chu, T. Bleicker, Sebastian Brünink, J. Schneider, Marie L Schmidt, D. Mulders, B. Haagmans, B. van der Veer, Sharon van den Brink, Lisa A. Wijsman, G. Goderski, J. Romette, J. Ellis, M. Zambon, M. Peiris, H. Goossens, C. Reusken, M. Koopmans, C. Drosten   +23 more
semanticscholar   +1 more source

Ultrafast Real-Time PCR in Photothermal Microparticles

ACS Nano, 2022
As the turnaround time of diagnosis becomes important, there is an increasing demand for rapid, point-of-care testing (POCT) based on polymerase chain reaction (PCR), the most reliable diagnostic tool.
Bong Kyun Kim, Sang-A Lee, Minju Park, Eui Ju Jeon, Mi Jung Kim, Jung Min Kim, Heesuk Kim, Seungwon Jung, Sang Kyung Kim   +8 more
semanticscholar   +1 more source

Enhanced analysis of real-time PCR data by using a variable efficiency model : FPK-PCR [PDF]

, 2011
Current methodology in real-time Polymerase chain reaction (PCR) analysis performs well provided PCR efficiency remains constant over reactions. Yet, small changes in efficiency can lead to large quantification errors. Particularly in biological samples,
Goetghebeur, Els, Lievens, Antoon, Van Aelst, Stefan, Van den Bulcke, Marc   +3 more
core   +3 more sources

MicroRNA and histopathological characterization of pure mucinous breast carcinoma

Cancer Biology & Medicine, 2013
Objective: To evaluate the short-term outcomes of video-assisted thoracic surgery (VATS) for thoracic tumors. Methods: The data of 1,790 consecutive patients were retrospectively reviewed.
Fang Li, Ning Li, Bai-Hua Zhang, Da-Wei Wang, Ke-Lin Sun, Gui-Yu Chen, Ju-Wei Mu, You-Sheng Mao et al.   +7 more
doaj   +1 more source

Prevalence and geographic distribution of Babesia conradae and detection of Babesia vogeli in free-ranging California coyotes (Canis latrans)

International Journal for Parasitology: Parasites and Wildlife, 2022
Babesia species are intraerythrocytic piroplasms that can result in disease characterized by hemolytic anemia and thrombocytopenia. Of the 5 species that are known to infect canids in the United States, Babesia conradae is most frequently diagnosed in ...
Nadia N. Javeed, Laura Shultz, Samantha Barnum, Janet E. Foley, Emir Hodzic, Emily L. Pascoe, Beatríz Martínez-López, Niamh Quinn, Danielle Bucklin, Jonathan D. Dear   +9 more
doaj   +1 more source

Handheld real-time PCR device

Lab on a Chip, 2016
World's smallest, fully autonomous, handheld real-time PCR was shown in this contribution. The device can quickly process up to four samples at a time with detection capability of a single DNA copy. The fully integrated system includes all required electronics for fluorescence measurement, data viewing (LCD display) and processing, and is ideal for use
Ahrberg, Christian D., Ilic, Bojan Robert, Manz, Andreas, Neužil, Pavel   +3 more
openaire   +4 more sources

Absolute quantification by droplet digital PCR versus analog real-time PCR

Nature Methods, 2013
Nanoliter-sized droplet technology paired with digital PCR (ddPCR) holds promise for highly precise, absolute nucleic acid quantification. Our comparison of microRNA quantification by ddPCR and real-time PCR revealed greater precision (coefficients of ...
Christopher M. Hindson, J. Chevillet, H. Briggs, Emily N. Gallichotte, I. K. Ruf, B. Hindson, R. Vessella, M. Tewari   +7 more
semanticscholar   +1 more source

Detection of Tumor Cell-Specific mRNA in the Peripheral Blood of Patients with Breast Cancer-Evaluation of Several Markers with Real-Time Reverse Transcription-PCR [PDF]

, 2013
It is widely known that cells from epithelial tumors, e. g., breast cancer, detach from their primary tissue and enter blood circulation. We show that the presence of circulating tumor cells (CTCs) in samples of patients with primary and metastatic ...
Alexandra Kölbl, Alix-Panabieres, Balbin, Bergqvist, Bernard, Bolke, Braun, Braun, Chang, Christian Schindlbeck, De Cremoux, Dontu, Dydensborg, Fabre-Lafay, Fleming, Frasor, Gervasoni, Giani, Guo, Hedenfalk, Inaba, Jiang, Julia Neugebauer, Kim, Klaus Friese, Konecny, Krawczyk, Kurec, Lafleur, Livak, Markou, Matthias Ilmer, Mendoza, Mitas, Moliterni, Nikseresht, Noack, Pan, Pantel, Ring, Selvamurugan, Simone Hofmann, Slamon, Smerage, Solomayer, Stefan Hutter, Tai, Udo Jeschke, Ulrich Andergassen, Vandesompele, Verena Engelstädter, Wang, Weissenstein, Wu, Wu, Zhang   +55 more
core   +3 more sources