Results 11 to 20 of about 3,663,569 (290)

Validation of the NAT Chagas IVD Kit for the Detection and Quantification of Trypanosoma cruzi in Blood Samples of Patients with Chagas Disease

Life, 2023
In the absence of validated biomarkers to control the cure of Chagas disease, PCR-based diagnosis is being used as the main tool for an early indication of therapeutic failure.
Otacilio C. Moreira, Alice Gomes Fernandes, Natalia Lins da Silva Gomes, Carolina Messias dos Santos, Thiago Jacomasso, Alexandre Dias Tavares Costa, Lucas de O. Rossetti Nascimento, Alejandro Marcel Hasslocher-Moreno, Pedro Emmanuel Alvarenga Americano do Brasil, Luis Gustavo Morello, Fabricio Klerynton Marchini, Marco Aurelio Krieger, Constança Britto   +12 more
doaj   +1 more source

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 2020
Background The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially ...
V. Corman, O. Landt, M. Kaiser, R. Molenkamp, A. Meijer, D. Chu, T. Bleicker, Sebastian Brünink, J. Schneider, Marie L Schmidt, D. Mulders, B. Haagmans, B. van der Veer, Sharon van den Brink, Lisa A. Wijsman, G. Goderski, J. Romette, J. Ellis, M. Zambon, M. Peiris, H. Goossens, C. Reusken, M. Koopmans, C. Drosten   +23 more
semanticscholar   +1 more source

Ultrafast Real-Time PCR in Photothermal Microparticles

ACS Nano, 2022
As the turnaround time of diagnosis becomes important, there is an increasing demand for rapid, point-of-care testing (POCT) based on polymerase chain reaction (PCR), the most reliable diagnostic tool.
Bong Kyun Kim, Sang-A Lee, Minju Park, Eui Ju Jeon, Mi Jung Kim, Jung Min Kim, Heesuk Kim, Seungwon Jung, Sang Kyung Kim   +8 more
semanticscholar   +1 more source

MicroRNA and histopathological characterization of pure mucinous breast carcinoma

Cancer Biology & Medicine, 2013
Objective: To evaluate the short-term outcomes of video-assisted thoracic surgery (VATS) for thoracic tumors. Methods: The data of 1,790 consecutive patients were retrospectively reviewed.
Fang Li, Ning Li, Bai-Hua Zhang, Da-Wei Wang, Ke-Lin Sun, Gui-Yu Chen, Ju-Wei Mu, You-Sheng Mao et al.   +7 more
doaj   +1 more source

Prevalence and geographic distribution of Babesia conradae and detection of Babesia vogeli in free-ranging California coyotes (Canis latrans)

International Journal for Parasitology: Parasites and Wildlife, 2022
Babesia species are intraerythrocytic piroplasms that can result in disease characterized by hemolytic anemia and thrombocytopenia. Of the 5 species that are known to infect canids in the United States, Babesia conradae is most frequently diagnosed in ...
Nadia N. Javeed, Laura Shultz, Samantha Barnum, Janet E. Foley, Emir Hodzic, Emily L. Pascoe, Beatríz Martínez-López, Niamh Quinn, Danielle Bucklin, Jonathan D. Dear   +9 more
doaj   +1 more source

Handheld real-time PCR device

Lab on a Chip, 2016
World's smallest, fully autonomous, handheld real-time PCR was shown in this contribution. The device can quickly process up to four samples at a time with detection capability of a single DNA copy. The fully integrated system includes all required electronics for fluorescence measurement, data viewing (LCD display) and processing, and is ideal for use
Ahrberg, Christian D., Ilic, Bojan Robert, Manz, Andreas, Neužil, Pavel   +3 more
openaire   +4 more sources

Absolute quantification by droplet digital PCR versus analog real-time PCR

Nature Methods, 2013
Nanoliter-sized droplet technology paired with digital PCR (ddPCR) holds promise for highly precise, absolute nucleic acid quantification. Our comparison of microRNA quantification by ddPCR and real-time PCR revealed greater precision (coefficients of ...
Christopher M. Hindson, J. Chevillet, H. Briggs, Emily N. Gallichotte, I. K. Ruf, B. Hindson, R. Vessella, M. Tewari   +7 more
semanticscholar   +1 more source

REAL TIME PCR;

The Professional Medical Journal, 2012
In recent years, real-time PCR has come forward as a robust and widely used molecular technique in clinical and biologicalsettings. Although it can detect very minute quantities of target nucleic acid, but quantification of specific nucleic acids is not an easy task.Accurate and precise quantification is hampered by a number of factors that may include
ABIDA RAZA, NAUREEN A KHATTAK
openaire   +3 more sources

Simulation of between repeat variability in real time PCR reactions. [PDF]

PLoS ONE, 2012
While many decisions rely on real time quantitative PCR (qPCR) analysis few attempts have hitherto been made to quantify bounds of precision accounting for the various sources of variation involved in the measurement process.
Antoon Lievens, Stefan Van Aelst, Marc Van den Bulcke, Els Goetghebeur   +3 more
doaj   +1 more source

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Genome Biology, 2002
BackgroundGene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes.
J. Vandesompele, K. De Preter, F. Pattyn, B. Poppe, N. Van Roy, A. De Paepe, F. Speleman   +6 more
semanticscholar   +1 more source