Results 21 to 30 of about 3,663,569 (290)

Optimization of primer sets and detection protocols for SARS-CoV-2 of coronavirus disease 2019 (COVID-19) using PCR and real-time PCR

open access: yesExperimental and Molecular Medicine, 2020
SARS-CoV-2 is very contagious and has rapidly spread globally. Due to various symptomatic and asymptomatic cases and the possibility of asymptomatic transmission, there is a pressing need for a fast and sensitive detection protocol to diagnose ...
Myungsun Park   +3 more
semanticscholar   +1 more source

Serology versus Real Time PCR in the Diagnosis of Human Brucellosis

open access: yesJournal of Pure and Applied Microbiology, 2017
With the unwelcome re-emergence of brucellosis in different regions of the world, an accurate and timely diagnosis of this zoonosis has become a daunting challenge.
Sara El-Hossain Aly Reda   +3 more
doaj   +1 more source

PCR REAL TIME PER L’IDENTIFICAZIONE DI MYCOBACTERIUM TUBERCULOSIS MDR

open access: yesMicrobiologia Medica, 2003
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F. Meacci   +5 more
doaj   +1 more source

A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything

open access: yesFrontiers in Microbiology, 2017
Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety.
P. Kralik, M. Ricchi
semanticscholar   +1 more source

Advanced and conventional molecular techniques in the diagnosis of Mycoplasma pneumoniae in patients with respiratory tract infection

open access: yesمجلة كلية الطب, 2014
Background:-M. pneumoniae is an important human pathogen that produces community-acquired respiratory tract infection. Diagnosis of M. pneumoniae infection is challenging and crucial for the timely initiation of the effective antibiotic therapy ...
Muntaha M. Hassan   +3 more
doaj   +1 more source

Rapid and Sensitive Qualitative Duoplex Real-Time PCR Method for Discriminatory and Confirmatory Diagnosis of HTLV-1 and HTLV-2 Infections: Brazilian Multicentric Study

open access: yesFrontiers in Medicine, 2022
Human T cell lymphotropic virus (HTLV) is the caustive agent of two main conditions i. e., the HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and the adult T-cell leukemia/lymphoma (ATLL).
Mauricio Cristiano Rocha-Junior   +23 more
doaj   +1 more source

Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1 [PDF]

open access: yesVeterinarski Glasnik, 2010
Examinations were performed on 65 samples of nasal smeas taken from calves and young cows with clinical symptoms of respiratory infection to determine the presence of the bovine herpes virus 1 using parallel implementation of molecular and standard ...
Milić Nenad   +6 more
doaj   +1 more source

Protein and mRNA expression of estradiol receptors during estrus in yaks (Bos grunniens)

open access: yesRevista Brasileira de Zootecnia, 2015
The objective of this study was to investigate mRNA by real-time PCR and protein expression by immunofluorescence of the estradiol receptors (ER) in the pineal gland, hypothalamus, pituitary gland, and gonads of yaks (Bos grunniens).
Shengdong Huo   +5 more
doaj   +1 more source

Development of New Modified Simple Polymerase Chain Reaction and Real-time Polymerase Chain Reaction for the Identification of Iranian Brucella abortus Strains [PDF]

open access: yesArchives of Razi Institute, 2019
Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified as biological threat agents.
S. Alamian   +4 more
doaj   +1 more source

Statistical analysis of real-time PCR data

open access: yesBMC Bioinformatics, 2006
Background Even though real-time PCR has been broadly applied in biomedical sciences, data processing procedures for the analysis of quantitative real-time PCR are still lacking; specifically in the realm of appropriate statistical treatment.
Reed Ann   +3 more
doaj   +1 more source

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