The Professional Medical Journal, 2012 In recent years, real-time PCR has come forward as a robust and widely used molecular technique in clinical and biologicalsettings. Although it can detect very minute quantities of target nucleic acid, but quantification of specific nucleic acids is not an easy task.Accurate and precise quantification is hampered by a number of factors that may include
ABIDA RAZA, NAUREEN A KHATTAK
openaire +3 more sources
Comparison of immunoassay and real-time PCR methods for the detection of Jembrana disease virus infection in Bali cattle [PDF]
, 2009 A sensitive diagnostic assay for the detection of infections with the bovine lentivirus Jembrana disease virus (JDV) is required in Indonesia to control the spread of Jembrana disease. Immunoassays are used routinely but are compromised by cross-reactive
Desport, M. +5 more
core +2 more sources
Sensitive and specific detection of Trypanosoma cruzi DNA in clinical specimens using a multi-target real-time PCR approach [PDF]
, 2012 Background: The laboratory diagnosis of Chagas disease is challenging because the usefulness of different diagnostic tests will depend on the stage of the disease.
Aznar, Christine +5 more
core +4 more sources
Genome Biology, 2002 BackgroundGene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high-throughput and accurate expression profiling of selected genes.
J. Vandesompele +6 more
semanticscholar +1 more source
Recent sequence variation in probe binding site affected detection of respiratory syncytial virus group B by real-time RT-PCR [PDF]
, 2017 Background Direct immuno-fluorescence test (IFAT) and multiplex real-time RT-PCR have been central to RSV diagnosis in Kilifi, Kenya. Recently, these two methods showed discrepancies with an increasing number of PCR undetectable RSV-B viruses ...
Agoti, Charles N. +8 more
core +2 more sources
Experimental and Molecular Medicine, 2020 SARS-CoV-2 is very contagious and has rapidly spread globally. Due to various symptomatic and asymptomatic cases and the possibility of asymptomatic transmission, there is a pressing need for a fast and sensitive detection protocol to diagnose ...
Myungsun Park +3 more
semanticscholar +1 more source
Serology versus Real Time PCR in the Diagnosis of Human Brucellosis
Journal of Pure and Applied Microbiology, 2017 With the unwelcome re-emergence of brucellosis in different regions of the world, an accurate and timely diagnosis of this zoonosis has become a daunting challenge.
Sara El-Hossain Aly Reda +3 more
doaj +1 more source
Comparison of subgingival bacterial sampling with oral lavage for detection and quantification of periodontal pathogens by real-time polymerase chain reaction [PDF]
, 2007 Background: Saliva has been studied for the presence of subgingival pathogens in periodontitis patients. With the anaerobic culture technique, the discrepancy between salivary recovery and subgingival presence has been significant, which makes this ...
Boutaga, Khalil +3 more
core +2 more sources
PCR for the detection of pathogens in neonatal early onset sepsis. [PDF]
, 2020 BACKGROUND: A large proportion of neonates are treated for presumed bacterial sepsis with broad spectrum antibiotics even though their blood cultures subsequently show no growth. This study aimed to investigate PCR-based methods to identify pathogens not
Bedford Russell, A +8 more
core +1 more source
A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything
Frontiers in Microbiology, 2017 Real time PCR (quantitative PCR, qPCR) is now a well-established method for the detection, quantification, and typing of different microbial agents in the areas of clinical and veterinary diagnostics and food safety.
P. Kralik, M. Ricchi
semanticscholar +1 more source