Results 181 to 190 of about 13,945 (210)

Regulation of muscarinic M2 receptors

Life Sciences, 1997
The molecular mechanisms involved in the regulation of muscarinic receptor gene expression are poorly understood. In an effort to gain a better understanding of the regulation of M2 receptors, we have investigated homologous and heterologous regulation of M2 muscarinic receptor protein and gene expression in human embryonic lung fibroblasts (HEL 299 ...
P J, Barnes, E B, Haddad, J, Rousell
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m2-Toxin: A Selective Ligand for M2 Muscarinic Receptors

Molecular Pharmacology, 1999
Selective ligands are needed for distinguishing the functional roles of M2 receptors in tissues containing several muscarinic receptor subtypes. Because the venom of the green mamba Dendroaspis angusticeps contains the most specific antagonists known for M1 and M4 receptors (m1-toxin and m4-toxin), it was screened for toxins that inhibit the binding of
J M, Carsi, H H, Valentine, L T, Potter
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Kinetic and biophysical analysis of the m2 muscarinic receptor

Life Sciences, 1995
The recombinant Pm2 muscarinic receptor expressed in Chinese hamster ovary (CHO) cells was used as a model system to examine receptor-effector coupling and ligand binding. In CHO cells, equilibrium binding studies and the dependence on receptor number per cell of the maximum response and EC50 values for agonist stimulation of phosphatidylinositol ...
B T, Hirschberg, V A, Mosser, G L, Peterson, A, Toumadje, W K, Vogel, W C, Johnson, M I, Schimerlik   +6 more
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A model of the human M2 muscarinic acetylcholine receptor

Journal of Computer-Aided Molecular Design, 2002
The M2 muscarinic acetylcholine receptor belongs to the family of rhodopsin like G-Protein Coupled Receptors. This subtype of muscarinic receptors is of special interest because it bears, aside from an orthosteric binding site, also an allosteric binding site.
Kirstin Jöhren, Hans-Dieter Höltje
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Amplification of the rat m2 muscarinic receptor gene by the polymerase chain reaction: Functional expression of the M2 muscarinic receptor

Life Sciences, 1990
A selective amplification of the coding sequence of the rat M2 muscarinic receptor gene was achieved by the polymerase chain reaction. The error rate of this amplification system under conditions specified was 1 nucleotide substitution in 841 base pairs.
J, Lai, J W, Bloom, H I, Yamamura, W R, Roeske   +3 more
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Evidence of paired M2 muscarinic receptors.

Molecular Pharmacology, 1991
Binding assays involving various antagonists, including N-[3H] methylscopolamine, [3H]quinuclidinyl benzilate, AFDX-116, pirenzepine, and propylbenzilylcholine mustard, disclosed only a single population of M2 muscarinic receptors in membranes from the rat "brainstem" (medulla, pons, and colliculi).
L T, Potter, L A, Ballesteros, L H, Bichajian, C A, Ferrendelli, A, Fisher, H E, Hanchett, R, Zhang   +6 more
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Immunohistochemical localization of muscarinic receptors (M2) in the rat skin

Cell and Tissue Research, 2000
Acetylcholine (ACh) produces pain when applied to human skin and excites cutaneous mechanoreceptors and nerve terminals. These effects are partially mediated by activation of muscarinic receptors. The expression of muscarinic receptor subtype M2 has been shown in sensory neurons of rat dorsal root ganglia using reverse transcriptase polymerase chain ...
R V, Haberberger, M, Bodenbenner
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A novel function of the M2 muscarinic receptor

Trends in Pharmacological Sciences
The M2 muscarinic receptor (M2R) is a prototypic class A G protein-coupled receptor (GPCR). Interestingly, Fasciani et al. recently identified an internal translation start site within the M2 receptor mRNA, directing the expression of a C-terminal receptor fragment.
Jürgen Wess, Liu Liu
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Diphenyl sulfoxides as selective antagonists of the muscarinic M2 receptor

Bioorganic & Medicinal Chemistry Letters, 2000
Structure activity studies on [4-(phenylsulfonyl)phenyl]methylpiperazine led to the discovery of 4-cyclohexyl-alpha-[4-[[4-methoxyphenyl(S)-sufinyl]phenyl]-1-pi perazineacetonitrile, 1, an M2 selective muscarinic antagonist. Affinity at the cloned human M2 receptor was 2.7 nM; the M1/M2 selectivity is 40-fold.
J A, Kozlowski, D B, Lowe, H S, Guzik, G, Zhou, V B, Ruperto, R A, Duffy, R, McQuade, G, Crosby, L A, Taylor, W, Billard, H, Binch, J E, Lachowicz   +11 more
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Cholesterol as a determinant of cooperativity in the M2 muscarinic cholinergic receptor

Biochemical Pharmacology, 2007
M2 muscarinic receptor extracted from Sf9 cells in cholate-NaCl differs from that extracted from porcine sarcolemma. The latter has been shown to exhibit an anomalous pattern in which the capacity for N-[3H]methylscopolamine (NMS) is only 50% of that for [3H]quinuclidinylbenzilate (QNB), yet unlabeled NMS exhibits high affinity for all of the sites ...
Alejandro T, Colozo, Paul S-H, Park, Chi Shing, Sum, Luca F, Pisterzi, James W, Wells   +4 more
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