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Somatomedin Action and Tissue Growth Factor Receptors
1987The somatomedins (SMs) are a family of peptide hormones which are growth hormone (GH)-dependent, possess insulin-like activity in extra-skeletal tissue, and promote the incorporation of (35-S)-sulfate into proteoglycans of cartilage. Although their precise role in the control of cellular growth and replication is still unclear, a growing body of ...
Ron G. Rosenfeld +3 more
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Characterization of the binding of a rat somatomedin to receptors in human placental cell membranes
Biochemical and Biophysical Research Communications, 1979Abstract The somatomedins presumably initiate their growth promoting effects by first binding to specific cell surface receptors in responsive tissues. The specific and high affinity binding of [125I]-rat somatomedin to human placental membranes was saturable and reversible with a dissociation constant of 4.5 × 10−9 M calculated from Scatchard ...
J W, Jacobs +3 more
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Mouse Placental Receptor for Basic Somatomedin following Maternal Ethanol Administration
Developmental Pharmacology and Therapeutics, 2017We have previously reported a reduction in the basic somatomedin (B-SM) binding activity of day-15 mouse placental membranes following 3 days of acute maternal ethanol administration. In the present experiments, we have investigated the effects of acute maternal ethanol administration early in gestation on the subsequent development of the placental B ...
L A, Kennedy, S, Mukerji, B, Bhaumick
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Characterization of a Specific Somatomedin-C Receptor on Isolated Bovine Growth Plate Chondrocytes*
Endocrinology, 1983The interaction of somatomedin (Sm) with growth plate chondrocytes (GPCs) is believed to be the primary stimulus of skeletal growth. Using techniques designed to disrupt as little as possible the phenotypic characteristics of GPCs, we have been able to obtain 3-4 x 10(8) viable cells from the major physes of one newborn calf.
S B, Trippel +3 more
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Affinity-Labeled Somatomedin-C Receptors and Binding Proteins from the Human Fetus*
The Journal of Clinical Endocrinology & Metabolism, 1984We investigated the ontogeny of the human placental membrane somatomedin-C (Sm-C)/insulin-like growth factor I (IGF-I) receptor by affinity labeling with the cross-linking agent disuccinimidyl suberate (DSS). Specific Sm-C receptors, identified from as early as 6 weeks gestation, demonstrated no apparent structural changes through the course of ...
J D, Grizzard +4 more
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1975
Publisher Summary This chapter presents the explorations of the insulinlike and growth-promoting properties of somatomedin by membrane receptor assays. A competitive radioreceptor assay using 125 I-labeled insulin and a human placental cell membrane preparation was developed to measure the total insulinlike activity (ILA) during the fractionation of
J J, van Wyk +5 more
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Publisher Summary This chapter presents the explorations of the insulinlike and growth-promoting properties of somatomedin by membrane receptor assays. A competitive radioreceptor assay using 125 I-labeled insulin and a human placental cell membrane preparation was developed to measure the total insulinlike activity (ILA) during the fractionation of
J J, van Wyk +5 more
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The Journal of Clinical Endocrinology & Metabolism, 1980
No agreement exists concerning the necessity for extracting serum somatomedin (Sm) before radioreceptor assay (RRA) and RIA. We have developed a simplified system of Sm extraction which has permitted a quantitative comparison of native and extracted Sm. To 0.8 ml of a mixture of 87.5% ethanol and 12.5% 2 N HC1, 0.2 ml serum is added.
W H, Daughaday, I K, Mariz, S L, Blethen
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No agreement exists concerning the necessity for extracting serum somatomedin (Sm) before radioreceptor assay (RRA) and RIA. We have developed a simplified system of Sm extraction which has permitted a quantitative comparison of native and extracted Sm. To 0.8 ml of a mixture of 87.5% ethanol and 12.5% 2 N HC1, 0.2 ml serum is added.
W H, Daughaday, I K, Mariz, S L, Blethen
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Somatomedin-C Receptor Ontogeny and Levels in Porcine Fetal and Human Cord Serum
The Journal of Clinical Endocrinology & Metabolism, 1976The ontogeny of somatomedin receptors in tissues of fetal pigs and levels of somatomedin-C in fetal pig serum at various gestational ages and in human cord serum was investigated. Specific binding of 125I somatomedin-C by particulate membranes prepared from fetal organs from a variety of gestational ages almost always exceeds specific 125I insulin ...
A J, D'ercole +2 more
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Australian Journal of Experimental Biology and Medical Science, 1978
SummaryMultiplication‐stimulating activity (MSA) in the medium of cultured rat liver cells was purified 120‐fold by chromatography on Dowex 50W and Sephadex G 50. MSA was labelled with 125I‐iodine and bound to a human placental membrane receptor. An assay for somatomedin‐like receptor activity (SmLRA) in unextracted serum was based on the displacement ...
M W, Brinsmead, G C, Liggins
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SummaryMultiplication‐stimulating activity (MSA) in the medium of cultured rat liver cells was purified 120‐fold by chromatography on Dowex 50W and Sephadex G 50. MSA was labelled with 125I‐iodine and bound to a human placental membrane receptor. An assay for somatomedin‐like receptor activity (SmLRA) in unextracted serum was based on the displacement ...
M W, Brinsmead, G C, Liggins
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The Journal of Clinical Endocrinology & Metabolism, 1981
We have previously demonstrated that somatomedin (SM) can induce the loss of specific SMC receptors on cultured IM-9 lymphocytes in a time- and concentration-dependent manner. To investigate the acute regulation of SM binding under in vivo conditions, we have evaluated SM receptors on circulating mononuclear cells obtained from 12 hypopituitary dwarfs ...
R G, Rosenfeld +3 more
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We have previously demonstrated that somatomedin (SM) can induce the loss of specific SMC receptors on cultured IM-9 lymphocytes in a time- and concentration-dependent manner. To investigate the acute regulation of SM binding under in vivo conditions, we have evaluated SM receptors on circulating mononuclear cells obtained from 12 hypopituitary dwarfs ...
R G, Rosenfeld +3 more
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