Results 91 to 100 of about 6,607,627 (360)

CD47 promotes mitogen‐activated protein kinase and epithelial‐to‐mesenchymal transition molecular programs to drive prometastatic phenotypes in non‐small cell lung cancer

Molecular Oncology, EarlyView.
Beyond its role in immune evasion, this study identified that CD47 drives tumor‐intrinsic signaling in non‐small cell lung cancer (NSCLC). Transcriptomic profiling and functional studies revealed that CD47 regulates cell adhesion, migration, and metastasis through an ERK–EMT signaling axis.
Asa P.Y. Lau, Lilian G. Zhai, Ryunosuke Hoshi, Zackary Rousseau, Abraam Zakhary, Yin Fang Wu, Rola M. Saleeb, Heyu Ni, Kelsie L. Thu   +8 more
wiley   +1 more source

Experimental and In Silico Modelling Analyses of the Gene Expression Pathway for Recombinant Antibody and By-Product Production in NS0 Cell Lines [PDF]

, 2012
Monoclonal antibodies are commercially important, high value biotherapeutic drugs used in the treatment of a variety of diseases. These complex molecules consist of two heavy chain and two light chain polypeptides covalently linked by disulphide bonds ...
Martin, Elaine B., C Mark Smales, Sarah K. Spurgeon (128819), Mead, Emma J., Emma J. Mead (128810), Smales, C. Mark, Chiverton, Lesley M., Smales, Christopher Mark, Elaine B. Martin (128823), C. Mark Smales (128831), Spurgeon Sarah K., Spurgeon, Sarah K., Montague, Gary A.; id_orcid, Montague, Gary A, Smales C. Mark, Montague, Gary A., von der Haar Tobias, Smales, C Mark, Emma J Mead, Sarah K Spurgeon, Gary A Montague, Martin Elaine B., Gary A. Montague (128826), von der Haar, Tobias, Mead, Emma J, Lesley M. Chiverton (128814), Chiverton Lesley M., Elaine B Martin, Tobias von der Haar, Tobias von der Haar (80575), Mead Emma J., Montague Gary A., Lesley M Chiverton   +32 more
core   +1 more source

Improvement of the transient expression system for production of recombinant proteins in plants

Scientific Reports, 2018
An efficient and high yielding expression system is required to produce recombinant proteins. Furthermore, the transient expression system can be used to identify the localization of proteins in plant cells.
Tsuyoshi Yamamoto, Ken Hoshikawa, Kentaro Ezura, Risa Okazawa, S. Fujita, Miyo Takaoka, H. Mason, H. Ezura, K. Miura   +8 more
semanticscholar   +1 more source

Heterozygous loss‐of‐function alleles associate the conserved 3′‐5′ exoribonuclease EXOSC10 with hypersensitivity to the anticancer drug 5‐fluorouracil

Molecular Oncology, EarlyView.
EXOSC10, an essential nuclear RNA exosome‐associated 3′‐5′ exoribonuclease, is inhibited by the anticancer drug 5‐fluorouracil (5‐FU), and EXOSC10 depletion increases 5‐FU sensitivity. The colon‐cancer variant EXOSC10S402T, located in a proteolysis motif, is stable and nuclear but nonfunctional in vivo.
Radhika Sain, Yann Le Page, Frédéric Percevault, Emmanuelle Becker, Luc Negroni, Almudena Fernandez, Marta Cantero, Diego Muñoz‐Santos, Lluís Montoliu, Cyrille Garnier, Michael Primig   +10 more
wiley   +1 more source

Expression, intracellular targeting and purification of HIV Nef variants in tobacco cells [PDF]

, 2007
Background Plants may represent excellent alternatives to classical heterologous protein expression systems, especially for the production of biopharmaceuticals and vaccine components.
Buriani, Giampaolo, Nuttall, James, Marusic Carla, Lombardi Raffaele, Lombardi, Raffaele, Baschieri , Selene, Buriani Giampaolo, Eugenio Benvenuto, Lico Chiara, Baschieri Selene, Frigerio, Lorenzo, James Nuttall, Benvenuto Eugenio, Lorenzo Frigerio, Nuttall James, Giampaolo Buriani, Marusic, Carla, Selene Baschieri, Frigerio Lorenzo, Raffaele Lombardi, Benvenuto, Eugenio, Chiara Lico, Carla Marusic, Lico, Chiara   +23 more
core   +1 more source

SETDB1 knockdown boosts recombinant protein in CHO cells via epigenetic transcriptional silencing

AMB Express
Chinese hamster ovary (CHO) cells serve as the predominant mammalian expression system for recombinant protein production. However, clonal heterogeneity and instability in recombinant protein expression remain significant challenges.
Jiang-Tao Lu, Miao Zhang, Yan-Ping Gao, Lu-Lu Yang, Zhao-Ming Cui, Ming-Ming Han, Xi Zhang, Hong-Yan Xu, Xiao-Yin Wang, Le-Le Qiu, Zi-Chun Hua, Tian-Yun Wang, Yan-Long Jia   +12 more
doaj   +1 more source

Recombinant expression and immunological characterization of Burkholderia pseudomallei type Ⅲ secretion system protein BipD

陆军军医大学学报
Objective To express recombinant Burkholderia pseudomallei (B. pseudomallei) type Ⅲ secretion system BipD protein, prepare its polyclonal antibodies and verify their immunological traits.
NAN Dongqi, WEN Yuan, CHEN Jiangao
doaj   +1 more source

The recombinant expression systems for structure determination of eukaryotic membrane proteins

Protein & Cell, 2014
Eukaryotic membrane proteins, many of which are key players in various biological processes, constitute more than half of the drug targets and represent important candidates for structural studies.
Yuan He, Kan Wang, N. Yan
semanticscholar   +1 more source

Interaction of HS1BP3 with cortactin modulates TKS5 localisation, cell secretion and cancer malignancy

Molecular Oncology, EarlyView.
Here, we demonstrate that HS1BP3 interacts with Cortactin through a proline‐rich region (PRR3.1) and show that this interaction, and HS1BP3 itself, promote cancer cell proliferation and invasion. Inhibition of this interaction leads to build‐up of TKS5 in multivesicular endosomes and altered secretion of CD63 and CD9, providing an explanation for the ...
Arja Arnesen Løchen, Kristiane Søreng, Chiara Veroni, Laura Trachsel‐Moncho, Nagham Asp, Robin Gaupset, Lars Gustav Lyckander, Helene Knævelsrud, Lars Eftang, Anne Simonsen   +9 more
wiley   +1 more source

Design and implementation of a high yield production system for recombinant expression of peptides

Microbial Cell Factories, 2014
BackgroundMaking peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome.
V. Rodríguez, J. Asenjo, B. Andrews
semanticscholar   +1 more source