Results 71 to 80 of about 6,607,627 (360)

Leveraging ANXA1 to enhance recombinant protein yields in CHO cells: A UPR-Mediated bioprocessing approach

Synthetic and Systems Biotechnology
Chinese hamster ovary (CHO) cells undergo endoplasmic reticulum stress (ERS) during intensive recombinant protein production, triggering the unfolded protein response (UPR) to balance cell survival and protein output.
Qi Zhao, Hui-Jie Zhang, Ming-Ming Han, Jumai Abiti, Yan-Ju Dong, Jia-Ning Wang, Jiang-Tao Lu, Wen Wang, Xi Zhang, Shao-Lei Geng, Le-Le Qiu, Xiao-Yin Wang, Zi-Chun Hua, Tian-Yun Wang, Yan-Long Jia   +14 more
doaj   +1 more source

Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis

Applied Microbiology and Biotechnology, 2015
The oxygenation reactions catalyzed by cytochromes P450 (CYPs) play critical roles in plant natural products biosynthesis. At the same time, CYPs are one of most challenging enzymes to functionally characterize due to the difficulty of recombinantly ...
Naoki Kitaoka, Yisheng Wu, Meimei Xu, R. Peters   +3 more
semanticscholar   +1 more source

Expression, purification and characterization of recombinant crambin [PDF]

"Protein Engineering, Design and Selection", 1996
Crambin, a small hydrophobic protein (4.7 kDa and 46 residues), has been successfully expressed in Escherichia coli from an artificial, synthetic gene. Several expression systems were investigated. Ultimately, crambin was successfully expressed as a fusion protein with the maltose binding protein, which was purified by affinity chromatography.
L, Lobb, B, Stec, E K, Kantrowitz, A, Yamano, V, Stojanoff, O, Markman, M M, Teeter   +6 more
openaire   +2 more sources

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Cross-kingdom regulation of plant microRNAs: potential application in crop improvement and human disease therapeutics

Frontiers in Plant Science
Plant microRNAs (miRNAs) are small non-coding RNA molecules that usually negatively regulate gene expression at the post-transcriptional level. Recent data reveal that plant miRNAs are not limited to individual plants but can transfer across different ...
Lei Shi, Lei Shi, Chao Guo, Miaomiao Fang, Yingmei Yang, Fei Yin, Yuan Shen, Yuan Shen   +7 more
doaj   +1 more source

Characterization of a Novel BmαTX47 Toxin Modulating Sodium Channels: The Crucial Role of Expression Vectors in Toxin Pharmacological Activity

Toxins, 2014
Long-chain scorpion toxins with four disulfide bridges exhibit various pharmacological features towards the different voltage-gated sodium channel subtypes. However, the toxin production still remains a huge challenge.
Tian Li, Lingna Xu, Honglian Liu, Yawen He, Songping Liang, Wenxin Li, Yingliang Wu   +6 more
doaj   +1 more source

A comparison between the recombinant expression and chemical synthesis of a short cysteine-rich insecticidal spider peptide

Journal of Venomous Animals and Toxins including Tropical Diseases, 2015
BackgroundThe choice between heterologous expression versus chemical synthesis for synthesizing short cysteine-rich insecticidal peptides from arthropods may impact the obtainment of yields and well-folded bioactive molecules for scientific research ...
H. Clement, Vianey Flores, Elia Diego‐Garcia, L. Corrales-García, E. Villegas, G. Corzo   +5 more
semanticscholar   +1 more source

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source

Optimisation of the production of cathepsin L1 from a recombinant saccharomyces cerevisiae [PDF]

, 2002
Cathepsin L1 is a cysteine protease that has been previously isolated and functionally expressed in Saccharomyces cerevisiae. It has the potential to be employed as a vaccine for liver-fluke disease in cattle and other ruminants.
O'Donovan, Eimear C
core  

Signal peptides for recombinant protein secretion in bacterial expression systems

Microbial Cell Factories, 2018
The secretion of biotechnologically or pharmaceutically relevant recombinant proteins into the culture supernatant of a bacterial expression host greatly facilitates their downstream processing and significantly reduces the production costs.
R. Freudl
semanticscholar   +1 more source