Results 61 to 70 of about 6,607,627 (360)

METHODS FOR RECOMBINANT EXPRESSION AND FUNCTIONAL CHARACTERIZATION OF HUMAN CANNABINOID RECEPTOR CB2

Computational and Structural Biotechnology Journal, 2013
Cannabinoid receptor CB2 is a seven transmembrane-domain integral membrane protein that belongs to a large superfamily of G protein-coupled receptors (GPCR).
Alexei A. Yeliseev
doaj   +1 more source

A Novel Insecticidal Spider Peptide that Affects the Mammalian Voltage-Gated Ion Channel hKv1.5

Frontiers in Pharmacology, 2021
Spider venoms include various peptide toxins that modify the ion currents, mainly of excitable insect cells. Consequently, scientific research on spider venoms has revealed a broad range of peptide toxins with different pharmacological properties, even ...
Diana Alvarado, Samuel Cardoso-Arenas, Ligia-Luz Corrales-García, Ligia-Luz Corrales-García, Herlinda Clement, Iván Arenas, Pavel Andrei Montero-Dominguez, Timoteo Olamendi-Portugal, Fernando Zamudio, Agota Csoti, Jesús Borrego, Gyorgy Panyi, Ferenc Papp, Gerardo Corzo   +13 more
doaj   +1 more source

Bacterial expression of recombinant MyoXVIIIA domains [PDF]

Analytical Biochemistry, 2005
Myosin XVIII (MyoXVIII)1, the newest member of the myosin superfamily (1), consists of two subclasses MyoXVIIIA and MyoXVIIIB (1, 2). Myosin family members have a motor domain, one or more IQ motifs and c-terminal tails that usually form dimeric coiled-coils, each domain having critical roles in a variety of cell functions (1, 3).
Jacek, Szeliga, Jeremy, Jordan, Ching-Hui, Yang, Zvjezdana, Sever-Chroneos, Zissis C, Chroneos   +4 more
openaire   +2 more sources

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Expression and purification of an adenylation domain from a eukaryotic nonribosomal peptide synthetase: Using structural genomics tools for a challenging target

, 2010
Nonribosomal peptide synthetases (NRPSs) are large multimodular and multidomain enzymes that are involved in synthesising an array of molecules that are important in human and animal health.
Lee, TV, Arcus, VL, Lott, JS, Johnson, RD, Lott, J. Shaun, Vickery L. Arcus, Johnson, Richard D., Arcus, Vickery L., J. Shaun Lott, Richard D. Johnson, T. Verne Lee, Lee, T. Verne   +11 more
core   +1 more source

Engineering of the Recombinant Expression and PEGylation Efficiency of the Therapeutic Enzyme Human Thymidine Phosphorylase

Frontiers in Bioengineering and Biotechnology, 2021
Human thymidine phosphorylase (HsTP) is an enzyme with important implications in the field of rare metabolic diseases. Defective mutations of HsTP lead to mitochondrial neurogastrointestinal encephalomyopathy (MNGIE), a disease with a high unmet medical ...
Christos S. Karamitros, Catrina M. Somody, Giulia Agnello, Scott Rowlinson   +3 more
doaj   +1 more source

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

Recombinant expression and phenotypic screening of a bioactive cyclotide against α-synuclein-induced cytotoxicity in baker’s yeast

Angewandte Chemie, 2015
We report for the first time the recombinant expression of fully folded bioactive cyclotides inside live yeast cells by using intracellular protein trans-splicing in combination with a highly efficient split-intein. This approach was successfully used to
K. Jagadish, A. Gould, R. Borra, S. Majumder, Zahid Mushtaq, A. Shekhtman, J. Camarero   +6 more
semanticscholar   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

Expression of the murine cytomegalovirus glycoprotein H by recombinant vaccinia virus [PDF]

, 1994
The sequence of the gene encoding glycoprotein H (gH) of murine cytomegalovirus (MCMV) strain Smith was determined and compared with the sequence of the gH of MCMV strain K181. Transcriptional analysis showed that gH is encoded by a large mRNA of 5.0 kb,
Rapp, Maria, Koszinowski, Ulrich H., Lucin, Pero, Messerle, Martin, Loh, Lambert C.   +4 more
core