Results 41 to 50 of about 6,607,627 (360)

Metabolomic analysis of riboswitch containing E. coli recombinant expression system.

Molecular Biosystems, 2016
In this study we have employed metabolomics approaches to understand the metabolic effects of producing enhanced green fluorescent protein (eGFP) as a recombinant protein in Escherichia coli cells.
H. Muhamadali, Yun Xu, R. Morra, D. Trivedi, N. Rattray, N. Dixon, R. Goodacre   +6 more
semanticscholar   +1 more source

Optimization of the intron sequences combined with the CMV promoter increases recombinant protein expression in CHO cells

Scientific Reports
To meet the requirements of the biopharmaceutical industry, improving the yield of recombination therapeutic protein (RTP) from Chinese hamster ovary (CHO) cells is necessary.
Ting-Ting Xu, Yan-Fang Wang, Jing-Jia Yuan, Chun-Liu Mi, Shao-Lei Geng, Xiao-yin Wang, Tian-Yun Wang   +6 more
doaj   +1 more source

Recombinant expression and biological characterization of the antimicrobial peptide fowlicidin-2 in Pichia pastoris.

Experimental and Therapeutic Medicine, 2016
Fowlicidins are a group of cathelicidin antimicrobial peptides that were initially identified in chickens. Fowlicidin-2, which is composed of 31 amino acids, is widely expressed in the majority of tissues in chickens and has an important role in innate ...
Li-Wei Xing, Shi Tian, Wei Gao, Na Yang, Pei Qu, Di Liu, Jian Jiao, Jue Wang, Xingjun Feng   +8 more
semanticscholar   +1 more source

Recombinant Expression of Cyclotides Using Expressed Protein Ligation

, 2020
Cyclotides are naturally occurring microproteins (≈30 residues long) present in several families of plants. All cyclotides share a unique head-to-tail circular knotted topology containing three disulfide bridges forming a cystine knot topology. Cyclotides possess high stability to chemical, physical, and biological degradation and have been reported to
Maria Jose, Campbell, Jingtan, Su, Julio A, Camarero   +2 more
openaire   +3 more sources

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Expression and purification of recombinant human coagulation Factor VII fused to His-Tag through Gateway technology [PDF]

Background. Factor VII is a plasma glycoprotein that participates in the coagulation process leading to generation of fibrin. Construction, expression and purification of recombinant FVII fused to poly histidin tag through gateway technology ...
عدالتی فتح آباد, مهدی, امیری زاده, ناصر, حبیبی رودکنار, مهریار, حلبیان, راحله, ساکی, ساسان, مسروری, ناصر, محمدی روشنده, آمنه   +6 more
core   +1 more source

Novel and effective screening system for recombinant protein production in CHO cells

Scientific Reports
At present, biopharmaceuticals have received extensive attention from the society, among which recombinant proteins have a good growth trend and a large market share.
Junhe Zhang, Wenwen Yang, Liao Zhang, Wenqing Li, Xi Zhang, Xiaoyin Wang, Tianyun Wang   +6 more
doaj   +1 more source

Additivities for Soluble Recombinant Protein Expression in Cytoplasm of Escherichia coli

Fermentation
Recombinant protein expression in Escherichia coli is a fundamental technique in molecular biology and biotechnology. This review provides a comprehensive overview of various additivities to enhance the expression levels of soluble recombinant proteins ...
D. Atroshenko, E. P. Sergeev, D. Golovina, A. Pometun   +3 more
semanticscholar   +1 more source

Recombinant rhizopuspepsinogen. Expression, purification, and activation properties of recombinant rhizopuspepsinogens

Journal of Biological Chemistry, 1991
A cDNA clone, which contained the complete rhizopuspepsin structure and the putative proregion, was placed in three different Escherichia coli expression vectors for the synthesis of rhizopuspepsinogen (Rpg). Recombinant Rpgs which were expressed in the cytosol of E. coli as inclusion bodies (cRpg and tRpg) were not active.
Z, Chen, G, Koelsch, H P, Han, X J, Wang, X L, Lin, J A, Hartsuck, J, Tang   +6 more
openaire   +2 more sources

pH‐mediated activation of the lysosomal arginine sensor SLC38A9

FEBS Letters, EarlyView.
Cells monitor nutrient levels via the lysosomal transporter SLC38A9 to activate the mechanistic target of rapamycin complex 1 (mTORC1). This study reveals that SLC38A9 function is regulated by pH. We identified histidine 544 as a critical pH sensor that undergoes conformational changes to control amino acid efflux from lysosomes; therefore, it ...
Xuelang Mu, Ampon Sae Her, Tamir Gonen
wiley   +1 more source