Results 21 to 30 of about 6,607,627 (360)

Optimization of Expression Conditions, Purification and Identification of the Recombinant Cowpea Hemoglobin Lb Ⅱ in Escherichia coli

open access: yesShipin gongye ke-ji, 2023
Cowpea has a red nodule containing large amounts of bean hemoglobin. The protein is a good natural pigment and can be used as color agent in artificial meat.
Meng LIU   +3 more
doaj   +1 more source

Recombinant protein expression in biofilms

open access: yesAIMS Microbiology, 2019
Biofilm research is usually focused on the prevention or control of biofilm formation. Recently, the significance of the biofilm mode of growth in biotechnological applications received increased attention. Since biofilm reactors show many advantages over suspended cell reactors, especially in their higher biomass density and operational stability ...
Alexandra Soares   +3 more
openaire   +3 more sources

Recombinant human preproinsulin expression, purification and reaction with insulin autoantibodies in sera from patients with insulin-dependent diabetes mellitus [PDF]

open access: yes, 1993
A novel prokaryotic expression vector pGEX-6T was designed for high-level expression of recombinant fusion protein with a histidine-hexapeptide and glutathione-S-transferase at its N-terminus and the recombinant human preproinsulin at its C-terminus ...
Seißler, Jochen   +4 more
core   +1 more source

Impact of the Expression System on Recombinant Protein Production in Escherichia coli BL21

open access: yesFrontiers in Microbiology, 2021
Recombinant protein production for medical, academic, or industrial applications is essential for our current life. Recombinant proteins are obtained mainly through microbial fermentation, with Escherichia coli being the host most used. In spite of that,
G. Lozano Terol   +5 more
semanticscholar   +1 more source

The multifunctionality of expression systems in Bacillus subtilis: Emerging devices for the production of recombinant proteins

open access: yesExperimental biology and medicine, 2021
Bacillus subtilis is a successful host for producing recombinant proteins. Its GRAS (generally recognized as safe) status and its remarkable innate ability to absorb and incorporate exogenous DNA into its genome make this organism an ideal platform for ...
C. C. D. Souza   +3 more
semanticscholar   +1 more source

Expression of recombinant multi-coloured fluorescent antibodies in gor -/trxB- E. coli cytoplasm [PDF]

open access: yes, 2011
Background Antibody-fluorophore conjugates are invaluable reagents used in contemporary molecular cell biology for imaging, cell sorting and tracking intracellular events.
Durvasula, Ravi V.   +26 more
core   +1 more source

Improved Recombinant Expression of Maltogenic α-Amylase AmyM in Bacillus subtilis by Optimizing Its Secretion and NADPH Production

open access: yesFermentation, 2023
The maltose α-amylase AmyM from Bacillus stearothermophilus can be used for flour modification, baked goods preservation, and maltose production. Here, we optimized the recombinant expression of AmyM in Bacillus subtilis WB800 via several strategies.
Yudan Chen   +3 more
doaj   +1 more source

Biophysical Characterization of the Oligomeric States of Recombinant Immunoglobulins Type-M and Their C1q-Binding Kinetics by Biolayer Interferometry

open access: yesFrontiers in Bioengineering and Biotechnology, 2022
Immunoglobulins type-M (IgMs) are one of the first antibody classes mobilized during immune responses against pathogens and tumor cells. Binding to specific target antigens enables the interaction with the C1 complex which strongly activates the ...
Anne Chouquet   +8 more
doaj   +1 more source

Orally administered recombinant Lactobacillus expressing African swine fever virus antigens that induced immunity responses

open access: yesFrontiers in Microbiology, 2023
African swine fever (ASF) is a highly contagious, acute, febrile disease caused by the African swine fever virus (ASFV), with morbidity and mortality rates approaching 100% in domestic and wild swine, resulting in massive economic losses to the pig ...
Hongliang Zhang   +7 more
doaj   +1 more source

Genetic Tools and Techniques for Recombinant Expression in Thermophilic Bacillaceae

open access: yesMicroorganisms, 2018
Although Escherichia coli and Bacillus subtilis are the most prominent bacterial hosts for recombinant protein production by far, additional species are being explored as alternatives for production of difficult-to-express proteins.
E. B. Drejer   +3 more
semanticscholar   +1 more source

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