Results 11 to 20 of about 4,269,972 (308)

Perspective: A proposal on solutions of modern supply chain construction for lactoferrin

Journal of Dairy Science, 2023
: Lactoferrin is an iron-binding glycoprotein of the transferrin family that is found in most bodily fluids of mammals and has a variety of biological and beneficial functions, with great importance in health enhancement as a supplement for humans and ...
Ruoyu Mao, Xuanxuan Ma, Ya Hao, Guihong Pen, Xueling Zheng, Na Yang, Da Teng, Jianhua Wang   +7 more
doaj   +1 more source

Recombinant human preproinsulin expression, purification and reaction with insulin autoantibodies in sera from patients with insulin-dependent diabetes mellitus [PDF]

, 1993
A novel prokaryotic expression vector pGEX-6T was designed for high-level expression of recombinant fusion protein with a histidine-hexapeptide and glutathione-S-transferase at its N-terminus and the recombinant human preproinsulin at its C-terminus ...
Seißler, Jochen, Mauch, Ludwig, Walter, Michael, Northemann, Wolfgang, Berg, Heike   +4 more
core   +1 more source

Optimization of Expression Conditions, Purification and Identification of the Recombinant Cowpea Hemoglobin Lb Ⅱ in Escherichia coli

Shipin gongye ke-ji, 2023
Cowpea has a red nodule containing large amounts of bean hemoglobin. The protein is a good natural pigment and can be used as color agent in artificial meat.
Meng LIU, Congrui WANG, Bo LIU, Xiangzhong ZHAO   +3 more
doaj   +1 more source

Expression and Characterization of Recombinant Ecarin [PDF]

The Protein Journal, 2012
Abstract The snake venom protease ecarin from Echis carinatus was expressed in stable transfected CHO-S cells grown in animal component free cell culture medium. Recombinant ecarin (r-ecarin) was secreted from the suspension adapted Chinese Hamster Ovary (CHO-S) host cells as a pro-protein and activation to the mature form of r-ecarin ...
Jonebring, A., Lange, U., Bucha, E., Deinum, J., Elg, M., Lovgren, A.   +5 more
openaire   +3 more sources

Improved Recombinant Expression of Maltogenic α-Amylase AmyM in Bacillus subtilis by Optimizing Its Secretion and NADPH Production

Fermentation, 2023
The maltose α-amylase AmyM from Bacillus stearothermophilus can be used for flour modification, baked goods preservation, and maltose production. Here, we optimized the recombinant expression of AmyM in Bacillus subtilis WB800 via several strategies.
Yudan Chen, Qinglong Xin, Li Pan, Bin Wang   +3 more
doaj   +1 more source

Expression of recombinant multi-coloured fluorescent antibodies in gor ^-/trxB^- E. coli cytoplasm [PDF]

, 2011
Background Antibody-fluorophore conjugates are invaluable reagents used in contemporary molecular cell biology for imaging, cell sorting and tracking intracellular events.
Durvasula, Ravi V., Burchell, Joy Marilyn; id_orcid, Kang, Angray S., Anatoliy Markiv, Burchell, J, Durvasula, R.V., Beatson, R., Beatson Richard, Beatson, Richard; id_orcid, Kang Angray S, Markiv, A., Markiv Anatoliy, Kang, A.S., Richard Beatson, Burchell, J., Durvasula Ravi V, Ravi V Durvasula, Burchell, Joy; id_orcid, Durvasula, RV, Markiv, Anatoliy, Burchell Joy, Markiv, A, Angray S Kang, Beatson, Richard, Joy Burchell, Kang, AS, Beatson, R   +26 more
core   +1 more source

Biophysical Characterization of the Oligomeric States of Recombinant Immunoglobulins Type-M and Their C1q-Binding Kinetics by Biolayer Interferometry

Frontiers in Bioengineering and Biotechnology, 2022
Immunoglobulins type-M (IgMs) are one of the first antibody classes mobilized during immune responses against pathogens and tumor cells. Binding to specific target antigens enables the interaction with the C1 complex which strongly activates the ...
Anne Chouquet, Andrea J. Pinto, Julia Hennicke, Wai Li Ling, Isabelle Bally, Linda Schwaigerlehner, Nicole M. Thielens, Renate Kunert, Jean-Baptiste Reiser   +8 more
doaj   +1 more source

Recombinant expression of fungal oxidases for industrial application [PDF]

, 2005
Laccases catalyse the oxidation of a range of organic substrates coupled to the reduction of molecular oxygen to water. They are members of the ubiquitous blue multi-copper oxidase family.
Piscitelli, Alessandra
core   +1 more source

Maltose-binding protein enhances secretion of recombinant human granzyme B accompanied by in vivo processing of a precursor MBP fusion protein [PDF]

, 2010
Background: The apoptosis-inducing serine protease granzyme B (GrB) is an important factor contributing to lysis of target cells by cytotoxic lymphocytes.
Wels, Winfried, Pranav Oberoi, Jabulowsky, Robert Alexander, Benhar, Itai, Benjamin Dälken, Oberoi, Pranav, Robert A Jabulowsky, Itai Benhar, Winfried S Wels, Dälken, Benjamin   +9 more
core   +1 more source

Orally administered recombinant Lactobacillus expressing African swine fever virus antigens that induced immunity responses

Frontiers in Microbiology, 2023
African swine fever (ASF) is a highly contagious, acute, febrile disease caused by the African swine fever virus (ASFV), with morbidity and mortality rates approaching 100% in domestic and wild swine, resulting in massive economic losses to the pig ...
Hongliang Zhang, Saisai Zhao, Saisai Zhao, Haojie Zhang, Yu Shen, Peijun Zhang, Hu Shan, Xiulei Cai   +7 more
doaj   +1 more source