Results 31 to 40 of about 6,607,627 (360)

Large-Scale Recombinant Expression and Purification of Human Tyrosinase Suitable for Structural Studies

open access: yesPLoS ONE, 2016
Human tyrosinase (TYR) is a glycoprotein that initiates the first two reactions in the melanin biosynthesis pathway. Mutations in its encoding gene cause Oculocutaneous Albinism type I (OCA1), the most severe form of albinism, which is a group of ...
X. Lai   +3 more
semanticscholar   +1 more source

Recombinant expression of LFchimera antimicrobial peptide in a plant-based expression system and its antimicrobial activity against clinical and phytopathogenic bacteria

open access: yes, 2018
Antimicrobial peptides (AMPs) with their broad and selective antimicrobial activity and lowered risk of resistance development in microbial populations are promining as a new alternative candidate to conventional antibiotics.
Mahmood Chahardoli   +3 more
semanticscholar   +1 more source

Recombinant expression of Streptococcus pneumoniae capsular polysaccharides in Escherichia coli

open access: yesOpen Biology, 2016
Currently, Streptococcus pneumoniae is responsible for over 14 million cases of pneumonia worldwide annually, and over 1 million deaths, the majority of them children.
E. Kay   +4 more
semanticscholar   +1 more source

Strategies for Heterologous Expression, Synthesis, and Purification of Animal Venom Toxins

open access: yesFrontiers in Bioengineering and Biotechnology, 2022
Animal venoms are complex mixtures containing peptides and proteins known as toxins, which are responsible for the deleterious effect of envenomations. Across the animal Kingdom, toxin diversity is enormous, and the ability to understand the biochemical ...
Esperanza Rivera-de-Torre   +9 more
doaj   +1 more source

Recombinant protein expression in microbial systems [PDF]

open access: yesFrontiers in Microbiology, 2014
With the advent of recombinant DNA technology, expressing heterologous proteins in microorganisms rapidly became the method of choice for their production at laboratory and industrial scale. Bacteria, yeasts and other hosts can be grown to high biomass levels efficiently and inexpensively.
Rosano, German Leandro   +1 more
openaire   +5 more sources

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

open access: yesFrontiers in Bioengineering and Biotechnology, 2019
Infectious diseases, along with cancers, are among the main causes of death among humans worldwide. The production of therapeutic proteins for treating diseases at large scale for millions of individuals is one of the essential needs of mankind.
N. Tripathi, A. Shrivastava
semanticscholar   +1 more source

The yeast expression system for recombinant glycosyltransferases [PDF]

open access: yesGlycoconjugate Journal, 1999
Glycosyltransferases are increasingly being used for in vitro synthesis of oligosaccharides. Since these enzymes are difficult to purify from natural sources, expression systems for soluble forms of the recombinant enzymes have been developed. This review focuses on the current state of development of yeast expression systems.
Malissard, Martine   +2 more
openaire   +5 more sources

Efficient recombinant expression of SFTI-1 in bacterial cells using intein-mediated protein trans-splicing

open access: yesBiopolymers, 2016
We report for the first time the recombinant expression of bioactive wild‐type sunflower trypsin inhibitor 1 (SFTI‐1) inside E. coli cells by making use of intracellular protein trans‐splicing in combination with a high efficient split‐intein.
Yilong Li   +4 more
semanticscholar   +1 more source

Expression, characterization, and application of human-like recombinant gelatin

open access: yesBioresources and Bioprocessing
Gelatin is a product obtained through partial hydrolysis and thermal denaturation of collagen, belonging to natural biopeptides. With irreplaceable biological functions in the field of biomedical science and tissue engineering, it has been widely applied.
Xiaoping Song   +3 more
semanticscholar   +1 more source

Biotechnology Data Analysis Training with Jupyter Notebooks

open access: yesJournal of Microbiology & Biology Education, 2023
Biotechnology has experienced innovations in analytics and data processing. As the volume of data and its complexity grow, new computational procedures for extracting information are being developed. However, the rate of change outpaces the adaptation of
Ulf W. Liebal   +6 more
doaj   +1 more source

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