Results 51 to 60 of about 6,607,627 (360)

Structure-Activity Relationships of Bioengineered Heparin/Heparan Sulfates Produced in Different Bioreactors

Molecules, 2017
Heparin and heparan sulfate are structurally-related carbohydrates with therapeutic applications in anticoagulation, drug delivery, and regenerative medicine.
Ha Na Kim, John M. Whitelock, Megan S. Lord   +2 more
doaj   +1 more source

Recombinant Antimicrobial Peptide OaBac5mini Alleviates Inflammation in Pullorum Disease Chicks by Modulating TLR4/MyD88/NF-κB Pathway

Animals, 2023
Pullorum disease (PD), caused by Salmonella Pullorum (S. Pullorum), is a serious threat to the poultry industry worldwide. Antimicrobial peptides (AMPs) have drawn extensive attention as new-generation antibiotics because of their broad antimicrobial ...
Shanshan Shen, Fei Ren, Junping He, Jie Wang, Yawei Sun, Jianhe Hu   +5 more
doaj   +1 more source

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Maltose-binding protein enhances secretion of recombinant human granzyme B accompanied by in vivo processing of a precursor MBP fusion protein

, 2010
Background: The apoptosis-inducing serine protease granzyme B (GrB) is an important factor contributing to lysis of target cells by cytotoxic lymphocytes.
Wels, Winfried, Pranav Oberoi, Jabulowsky, Robert Alexander, Benhar, Itai, Benjamin Dälken, Oberoi, Pranav, Robert A Jabulowsky, Itai Benhar, Winfried S Wels, Dälken, Benjamin   +9 more
core   +1 more source

Automation of large scale transient protein expression in mammalian cells [PDF]

, 2011
Traditional mammalian expression systems rely on the time-consuming generation of stable cell lines; this is difficult to accommodate within a modern structural biology pipeline.
Susan Daenke, Jones, M, E. Yvonne Jones, Siebold, Christian, Daenke, Susan, Christian Siebold, Bishop, Benjamin, Margaret Jones, Clay, Jordan E, Stuart, David I., A. Radu Aricescu, Zhao, Y, Benjamin Bishop, Aricescu, Alexandru R, Radu Aricescu, A, Stuart, David, Yvonne Jones, E, Weixian Lu, Stuart, David I, Clay, JE, Daenke, S, Aricescu, Alexandru, Bishop, B, Jones, EY, Clay, Jordan E., Yuguang Zhao, Jones, Margaret, Lu, Weixian, David I. Stuart, Radu Aricescu, A., Yvonne Jones, E., Aricescu, AR, Jones, Edith, Jordan E. Clay, Zhao, Yuguang, Jones, E Yvonne, Lu, W, Siebold, C, Stuart, DI   +38 more
core   +1 more source

Production of Recombinant Proteins by Agrobacterium-Mediated Transient Expression

, 2022
The agroinfiltration of plant tissue is a robust method that allows the rapid and transient expression of recombinant proteins. Using wild-type plants as biomass, agroinfiltration exploits the ability of plants to synthesize even complex multimeric ...
Schillberg, Stefan, Noelke, Greta, Nölke, Greta, Spiegel, Holger   +3 more
core   +1 more source

Exploring the Utility of Recombinant Snake Venom Serine Protease Toxins as Immunogens for Generating Experimental Snakebite Antivenoms

Toxins, 2022
Snakebite is a neglected tropical disease that causes high rates of global mortality and morbidity. Although snakebite can cause a variety of pathologies in victims, haemotoxic effects are particularly common and are typically characterised by ...
Nessrin Alomran, Patricia Blundell, Jaffer Alsolaiss, Edouard Crittenden, Stuart Ainsworth, Charlotte A. Dawson, Rebecca J. Edge, Steven R. Hall, Robert A. Harrison, Mark C. Wilkinson, Stefanie K. Menzies, Nicholas R. Casewell   +11 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Use of Recombinant Virus Replicon Particles for Vaccination against Mycobacterium ulcerans Disease. [PDF]

, 2015
Buruli ulcer, caused by infection with Mycobacterium ulcerans, is a necrotizing disease of the skin and subcutaneous tissue, which is most prevalent in rural regions of West African countries.
Kerber, Sarah, Zimmer, Gert, Miriam Bolz, Sarah Kerber (421868), Bolz, Miriam, Gerd Pluschke, Miriam Bolz (309717), Gert Zimmer, Sarah Kerber, Gerd Pluschke (7855), Pluschke, Gerd, Gert Zimmer (223023)   +11 more
core   +1 more source

Synthetic enhancers including TFREs improve transgene expression in CHO cells

Heliyon
The human cytomegalovirus major immediate early gene (CMV) promoter is currently the most preferred promoter for recombinant therapeutic proteins (RTPs) production in CHO cells.
Hui-Ning Liu, Xiao-Yin Wang, Ying Zou, Wen-Bao Wu, Yan Lin, Bo-Yu Ji, Tian-Yun Wang   +6 more
doaj   +1 more source