Results 111 to 120 of about 4,269,972 (308)
FEBS Open Bio, EarlyView.Lactoferrin promotes wound healing by activating MAPK, PI3K/Akt, and extracellular matrix pathways, enhancing fibroblast remodeling, proliferation, and re‐epithelialization. These mechanisms highlight its therapeutic potential in inflammation control, tissue repair, and remodeling.
Morgana Lüdtke Azevedo +4 more
wiley +1 more source
Recombinant Cytokines from Plants [PDF]
, 2011 Plant-based platforms have been successfully applied for the last two decades for the efficient production of pharmaceutical proteins. The number of commercialized products biomanufactured in plants is, however, rather discouraging. Cytokines are small
Sirko, Agnieszka +7 more
core +1 more source
BioTechHuman embryonic kidney (HEK293) cells are a widespread choice for recombinant protein expression. To optimise yields, the hydrolysate Tryptone N1 (TN1) is commonly added post-transfection. TN1 is obtained by controlled enzymatic digestion of casein.
Shafqat Shabir +4 more
doaj +1 more source
FEBS Open Bio, EarlyView.We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
BioTechPolyamines and their acetylated derivatives are promising biomarkers for noninvasive cancer diagnostics, creating demand for robust enzymatic tools for their detection.
Egor P. Sergeev, Denis L. Atroshenko
doaj +1 more source
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Multiple Strategies for Recombinant Expression of Human Chymotrypsinogen C in Pichia Pastoris
Food BioengineeringChymotrypsin C (CTRC) is a multifunctional serine protease that have shown great application potential in the field of food processing. This study aims to efficiently express CTRC in Pichia pastoris and provide a safe and highly effective new enzyme ...
Yi Liu +5 more
doaj +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Co-expression of Factor VIII with anti-FVIII Camelid antibody ligands: Effect on expression levels of bio-therapeutic FVIII [PDF]
, 2015 Production of recombinant FVIII, the protein that is missing or dysfunctional in haemophilia A patients, is highly inefficient compared to other recombinant clotting factors such as FIX.
Tolley, Caroline
core
Production of high-affinity glycosylated anti-mouse conjugated nanobodies in Pichia pastoris
Frontiers in Bioengineering and BiotechnologyIntroductionNanobodies (NBs) are small antibody fragments derived from camelid heavy-chain antibodies, which represent the minimal functional domain capable of antigen recognition and binding.
Sofía Orioli +7 more
doaj +1 more source