Results 111 to 120 of about 6,607,627 (360)

Recombinant Cytokines from Plants [PDF]

, 2011
Plant-based platforms have been successfully applied for the last two decades for the efficient production of pharmaceutical proteins. The number of commercialized products biomanufactured in plants is, however, rather discouraging. Cytokines are small
Sirko, Agnieszka, Patrycja Redkiewicz, Vanek, Tomas, Agnieszka Sirko, Góra-Sochacka, Anna, Redkiewicz, Patrycja, Anna Góra-Sochacka, Tomas Vaněk   +7 more
core   +1 more source

Exploring Use of a Protein Cage System for Producing Bioactive Peptides in Escherichia coli

Microbial Biotechnology
New therapeutics are urgently needed to curb the spread of drug‐resistant diseases. Bioactive peptides (BAPs), including antimicrobial peptides, are emerging as an exciting new class of compounds with advantages over current drug modalities, especially ...
Maxim D. Harding, Mark A. Jackson, Edward K. Gilding, Kuok Yap, David J. Craik, Frank Sainsbury, Nicole Lawrence   +6 more
doaj   +1 more source

Identification of a Novel Chitinase from Bacillus paralicheniformis: Gene Mining, Sequence Analysis, and Enzymatic Characterization

Foods
In this study, a novel strain for degrading chitin was identified as Bacillus paralicheniformis HL37, and the key chitinase CH1 was firstly mined through recombinant expression in Bacillus amyloliquefaciens HZ12.
Xianwen Ma, Dian Zou, Anying Ji, Cong Jiang, Ziyue Zhao, Xiaoqi Ding, Zongchen Han, Pengfei Bao, Kang Chen, Aimin Ma, Xuetuan Wei   +10 more
doaj   +1 more source

Advances in microbial antimicrobial peptides expression system and transformation strategy

Journal of Hebei University of Science and Technology, 2019
In this paper, a review on antimicrobial peptides, including the bioactivity, recombinant expression system and molecular transformation strategy, is provided.
Ke XU, Liping ZHANG, Yuanyuan ZHANG, Junguo LIU   +3 more
doaj   +1 more source

Recombinant expression and immunological characterization of hemolysin coregulated protein 1, Burkholderia pseudomallei type Ⅵ secretion system protein

Di-san junyi daxue xuebao, 2020
Objective To express recombinant hemolysin coregulated protein 1 (Hcp1) of type Ⅵ secretion system (T6SS) of Burkholderia pseudomallei and identify its immunological properties.
ZHANG Meijuan, HU Zhiqiang, XIA Yupei, YUAN Siqi, YAN Jingmin   +4 more
doaj   +1 more source

Expression of Recombinant Toxoplasma gondii P24.

Journal of Veterinary Medical Science, 1999
The gene encoding Toxoplasma gondii P24 has been reported previously. To determine the function of P24 against immune systems in the near future, we prepared recombinant P24 antigens using Escherichia coli, insect cells infected with recombinant baculovirus and mammalian cells infected with recombinant vaccinia virus.
TANAKA, Tetsuya, XUAN, Xuenan, KATO, Mihoko, NAGASAWA, Hideyuki, FUJISAKI, Kozo, MIKAMI, Takeshi, SUZUKI, Naoyoshi   +6 more
openaire   +2 more sources

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source

Evaluation of a reverse tetracycline inducible system in recombinant BCG to improve stability and immunogenicity

, 2011
The aim of this study is to utilise the tetracycline dependent gene regulation system to down-regulate recombinant antigen expression in BCG during the expansion of seed stocks and up-regulate expression prior to or post ...
Mbele, Prisca
core  

Expression of an anti-CD33 single-chain antibody by Pichia pastoris [PDF]

, 2005
CD33 is a cell surface glycoprotein expressed on cells of myelomonocytic lineage, leukaemic cells, but not haematopoietic stem cells. By virtue of its expression pattern, CD33 has become a popular target for new immunotherapeutic approaches to treat ...
Nicholls, Peter J., Blower, Philip J., Peter J. Nicholls, Trivett, Amanda J., Blower, P J, Emberson, L M, Philip J. Blower, Nicholls, P J, Amanda J. Trivett, Louise M. Emberson, Emberson, Louise M., Trivett, A J   +11 more
core   +1 more source

Inhibition of cyclin‐dependent kinases 12/13 using CT7439 as a treatment for colorectal cancer with CDK12 upregulation

Molecular Oncology, EarlyView.
The proposed mechanism of action for the CDK12/13 inhibitor and cyclin K degrader, CT7439. CDK12/13 inhibition interrupts transcription elongation, leading to increased DNA damage that results in cell death. This agent is a potentially novel treatment option for patients with colorectal cancer. Created in BioRender. Cyclin‐dependent kinase (CDK) 12 and
Wylie K. Watlington, Divya L. Dayanidhi, Mohammad Zokaasadi, John B. Mantyh, Pelumi D. Olawuni, Gabrielle Rupprecht, Jeremy M. Force, Shannon McCall, Ashwani K. Bahl, David S. Hsu, Jason A. Somarelli   +10 more
wiley   +1 more source