Results 41 to 50 of about 399,275 (342)

Genetic Structures and Conditions of their Expression, which Allow Receiving Native Recombinant Proteins with High Output [PDF]

International Journal of Biomedicine, 2012
We investigated the possibility of obtaining native recombinant amyloidogenic proteins by creating genetic constructs encoding fusion proteins of target proteins with Super Folder Green Fluorescent Protein (sfGFP).
Michael M. Shavlovsky, PhD, ScD¹, Irina V. Morozova¹, Dmitry S. Polyakov, PhD¹, Tatyana D. Aleynikova, PhD¹, Anna M. Kern², Natalya A. Grudinina, PhD¹, Kirill V. Solovyov, PhD¹   +6 more
doaj  

Effect of active immunization with recombinant-derived goose INH-α, AMH, and PRL fusion protein on broodiness onset and egg production in geese (Anser cygnoides)

Poultry Science, 2021
: This study was conducted to investigate the potential effects of active immunization against recombinant-derived goose inhibin-α (INH-α), anti-Müllerian hormone (AMH), and prolactin (PRL) fusion protein on broodiness onset and egg production in geese ...
Y. Zhang, Z.Y. Chen, C. An, K.Q. Weng, Z.F. Cao, Q. Xu, G.H. Chen   +6 more
doaj   +1 more source

The rab3A-22A chimera prevents sperm exocytosis by stabilizing open fusion pores [PDF]

, 2016
At the final stage of exocytotis, a fusion pore opens between the plasma and a secretory vesicle membranes; typically, when the pore dilates the vesicle releases its cargo.
Bustos, Matias Alberto, De la Iglesia, Paola X., Lucchesi, Ornella, Pocognoni, Cristián Adrián, Quevedo, María Florencia, Tomes, Claudia Nora   +5 more
core   +1 more source

Recombinant protein production data after expression in the bacterium Escherichia coli

Data in Brief, 2016
Fusion proteins have become essential for the expression and purification of recombinant proteins in Escherichia coli. The metal-binding protein CusF has shown several features that make it an attractive fusion protein and affinity tag: ''Expression and ...
J. Enrique Cantu-Bustos, Kevin D. Cano del Villar, Teresa Vargas-Cortez, Jose Ruben Morones-Ramirez, Isaias Balderas-Renteria, Xristo Zarate   +5 more
doaj   +1 more source

Complexity of the Ruminococcus flavefaciens FD-1 cellulosome reflects an expansion of family-related protein-protein interactions [PDF]

, 2017
This work was supported in part by the European Union, Area NMP.2013.1.1–2: Self-assembly of naturally occurring nanosystems: CellulosomePlus Project number: 604530, and by the EU Seventh Framework Programme (FP7 2007–2013) under the WallTraC project ...
Alves, Victor D, Barak, Yoav, Bayer, Edward A, Borovok, Ilya, Bule, Pedro, Cardoso, Vânia, Dassa, Bareket, Flint, Harry J, Fontes, Carlos M G A, Gilbert, Harry J, Hamberg, Yuval, Israeli-Ruimy, Vered, Jindou, Sadanari, Lamed, Raphael, Moraïs, Sarah, Najmudin, Shabir, Slutzki, Michal, White, Bryan A   +17 more
core   +1 more source

Development of a novel fusion system for soluble protein overexpression and purification in Escherichia coli [PDF]

, 2010
Recombinant protein production is a useful technology for therapeutic and diagnostic applications. The bacterium Escherichia coli is widely used for the bioproduction of proteins, but still presents some drawbacks.
Castro, António, Costa, Sofia Judite, Domingues, Lucília   +2 more
core  

The Caenorhabditis elegans DPF‐3 and human DPP4 have tripeptidyl peptidase activity

FEBS Letters, EarlyView.
The dipeptidyl peptidase IV (DPPIV) family comprises serine proteases classically defined by their ability to remove dipeptides from the N‐termini of substrates, a feature that gave the family its name. Here, we report the discovery of a previously unrecognized tripeptidyl peptidase activity in DPPIV family members from two different species.
Aditya Trivedi, Rajani Kanth Gudipati
wiley   +1 more source

Development of alkaline phosphatase-scFv and its use for one-step enzyme-linked immunosorbent assay for His-tagged protein detection

Open Life Sciences, 2022
A histidine (His)-tag is composed of six His residues and typically exerts little influence on the structure and solubility of expressed recombinant fusion proteins.
He Shuzhen, Xu Ruixian, Yi Huashan, Chen Zhixin, Chen Congjie, Li Qiang, Han Qinqin, Xia Xueshan, Song Yuzhu, Xu Junwei, Zhang Jinyang   +10 more
doaj   +1 more source

Experimental Spinal Fusion With Recombinant Human Bone Morphogenetic Protein-2 Without Decortication of Osseous Elements [PDF]

, 1997
Study Design. L4-L5 intertransverse process fusions were produced with 58 μg, 230 μg, or 920 μg of recombinant human bone morphogenetic protein-2 in 20 dogs. Eleven had traditional decortication of posterior elements before insertion of the implant. Nine
Dawson, Edgar G., Delamarter, Rick B., Kabo, J. Michael, Kanim, Linda E.A., Liu, David, Sandhu, Harvinder, Toth, Jeffrey M.   +6 more
core   +1 more source

Novel prokaryotic expression of thioredoxin-fused insulinoma associated protein tyrosine phosphatase 2 (IA-2), its characterization and immunodiagnostic application [PDF]

, 2016
Background The insulinoma associated protein tyrosine phosphatase 2 (IA-2) is one of the immunodominant autoantigens involved in the autoimmune attack to the beta-cell in Type 1 Diabetes Mellitus.
A Trabucchi, A Trabucchi, A Trabucchi, A Villalba, A Villalba, A Westerlund-Karlsson, Adriana Victoria Sabljic, AG Krochik, AL Notkins, Aldana Trabucchi, BD Spangler, Bruno David Rovitto, C Levy-Marchal, C Tiberti, C Torn, CA Baer, CC Richardson, CF Verge, D Barford, DU Rabin, DU Rabin, E Bonifacio, E Bonifacio, E Kawasaki, EA Gale, Edgardo Poskus, ELD Harlow, ER LaVallie, ER LaVallie, ET Mambetisaeva, Expert Committee on the Diagnosis and Classification of Diabetes Mellitus, F Baneyx, F Sodoyez-Goffaux, G Hannig, GS Eisenbarth, H Goetz, H Mziaut, H Mziaut, H Shägger, H Xie, I Kikkas, J Sambrook, J Seissler, J Suckale, JA Berzofsky, JA Berzofsky, JA Dromey, JC Sodoyez, JM Gonzalez-Buitrago, JM Wenzlau, JP Palmer, K Lobner, KT Elvers, L Castano, LL Guerra, Luciano Lucas Guerra, M Hawa, M Knip, M Masuda, M Solimena, M Trajkovski, M Trajkovski, MA Payton, ML Papouchado, ML Papouchado, MP Sica, MS Lan, MS Lan, Natalia Inés Faccinetti, NG Morgenthaler, O Pines, P Tompa, PD Burbelo, PJ Bingley, PZ Zimmet, R Gianani, R Turner, RD Hoffman, RR Stumpo, Ruben Francisco Iacono, S Chen, S Krause, S Schubert, S Torii, Silvina Noemí Valdez, SJ Kim, SLBP Thrower, SN Valdez, SN Valdez, T Ort, T Tuomi, VN Uversky, WA Hagopian, X Jia, X Palomer, Z Zhao   +95 more
core   +5 more sources