Results 41 to 50 of about 577,747 (248)

Generation of a vector suite for protein solubility screening

Frontiers in Microbiology, 2014
Recombinant protein expression has become an invaluable tool for academic and biotechnological projects. With the use of high-throughput screening technologies for soluble protein production, uncountable target proteins were produced in a soluble and ...
Agustin eCorrea, Claudia eOrtega, Gonzalo eObal, Pedro eAlzari, Renaud eVincentelli, Pablo Javier Oppezzo   +5 more
doaj   +1 more source

Study of native SMAC protein production in the pUbiq expression system: Molecular cloning, biosynthesis and molecular modelling

Electronic Journal of Biotechnology, 2022
Background: In the process of recombinant protein biosynthesis affinity tags are efficient tools to achieve the expected purity and yield during the purification steps.
Pál Salamon, Csongor K. Orbán, Katalin Molnár-Nagy, Zita Kovács, Klára Váncsa, Emese Bálint, Ildikó Miklóssy, Beáta Albert, Gyöngyi Tar, Szabolcs Lányi   +9 more
doaj  

The recombinant expression and activity detection of MAF-1 fusion protein [PDF]

Scientific Reports, 2015
AbstractThis study establishes the recombinant expression system of MAF-1 (Musca domesticaantifungal peptide-1) and demonstrates the antifungal activity of the expression product and shows the relationship between biological activity and structure. The gene segments on mature peptide part of MAF-1 were cloned, based on the primers designed according to
Jian Liu, Ping Fu, Song Gao, Yong Zhang, Jianwei Wu, Guo Guo   +5 more
openaire   +3 more sources

Genetic Structures and Conditions of their Expression, which Allow Receiving Native Recombinant Proteins with High Output [PDF]

International Journal of Biomedicine, 2012
We investigated the possibility of obtaining native recombinant amyloidogenic proteins by creating genetic constructs encoding fusion proteins of target proteins with Super Folder Green Fluorescent Protein (sfGFP).
Michael M. Shavlovsky, PhD, ScD¹, Irina V. Morozova¹, Dmitry S. Polyakov, PhD¹, Tatyana D. Aleynikova, PhD¹, Anna M. Kern², Natalya A. Grudinina, PhD¹, Kirill V. Solovyov, PhD¹   +6 more
doaj  

Automated production of recombinant human proteins as resource for proteome research

Proteome Science, 2008
Background An arbitrary set of 96 human proteins was selected and tested to set-up a fully automated protein production strategy, covering all steps from DNA preparation to protein purification and analysis.
Poustka Annemarie, Wiemann Stefan, Schmidt Christian, Kohl Thorsten, Korf Ulrike   +4 more
doaj   +1 more source

Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for Mammalian Protein Expression Systems. [PDF]

PLoS ONE, 2016
Recombinant proteins are commonly expressed in eukaryotic expression systems to ensure the formation of disulfide bridges and proper glycosylation. Although many proteins can be expressed easily, some proteins, sub-domains, and mutant protein versions ...
Raphael Reuten, Denise Nikodemus, Maria B Oliveira, Trushar R Patel, Bent Brachvogel, Isabelle Breloy, Jörg Stetefeld, Manuel Koch   +7 more
doaj   +1 more source

Analysis of autoantibody binding to different regions of the human La antigen expressed in recombinant fusion proteins.

Journal of Immunology, 1988
To determine the specificity of autoantibodies for various antigenic sites on a self-protein molecule, sera from 19 patients with anti-La antibodies were tested for their reactivity with molecularly cloned La protein fragments.
E. S. St Clair, D. Pisetsky, C. Reich, J. Keene   +3 more
semanticscholar   +1 more source

ACCUMULATION OF RECOMBINANT FUSION PROTEIN – SECRETORY ANALOG OF Ag85B AND ESAT6 MYCOBACTERIUM TUBERCULOSIS PROTEINS – IN TRANSGENIC Lemna minor L. PLANTS [PDF]

Biotechnologia Acta, 2015
Determination of the presence of the recombinant fusion protein (ESAT6-Ag85B(ΔTMD)-6His) and its accumulation level in duckweed plants (Lemna minor L.) was the aim of the research.
A.A.Peterson, M. Yu. Vasylenko, N. A. Matvieieva, M. V. Kuchuk   +3 more
doaj   +1 more source

Antigenicity and Immunogenicity Analysis of the E. coli Expressed FMDV Structural Proteins; VP1, VP0, VP3 of the South African Territories Type 2 Virus

Viruses, 2021
An alternative vaccine design approach and diagnostic kits are highly required against the anticipated pandemicity caused by the South African Territories type 2 (SAT2) Foot and Mouth Disease Virus (FMDV).
Guoxiu Li, Ashenafi Kiros Wubshet, Yaozhong Ding, Qian Li, Junfei Dai, Yang Wang, Qian Hou, Jiao Chen, Bing Ma, Anna Szczotka-Bochniarz, Susan Szathmary, Yongguang Zhang, Jie Zhang   +12 more
doaj   +1 more source

Development of alkaline phosphatase-scFv and its use for one-step enzyme-linked immunosorbent assay for His-tagged protein detection

Open Life Sciences, 2022
A histidine (His)-tag is composed of six His residues and typically exerts little influence on the structure and solubility of expressed recombinant fusion proteins.
He Shuzhen, Xu Ruixian, Yi Huashan, Chen Zhixin, Chen Congjie, Li Qiang, Han Qinqin, Xia Xueshan, Song Yuzhu, Xu Junwei, Zhang Jinyang   +10 more
doaj   +1 more source