Results 41 to 50 of about 577,747 (248)
Generation of a vector suite for protein solubility screening
Recombinant protein expression has become an invaluable tool for academic and biotechnological projects. With the use of high-throughput screening technologies for soluble protein production, uncountable target proteins were produced in a soluble and ...
Agustin eCorrea+5 more
doaj +1 more source
Background: In the process of recombinant protein biosynthesis affinity tags are efficient tools to achieve the expected purity and yield during the purification steps.
Pál Salamon+9 more
doaj
The recombinant expression and activity detection of MAF-1 fusion protein [PDF]
AbstractThis study establishes the recombinant expression system of MAF-1 (Musca domesticaantifungal peptide-1) and demonstrates the antifungal activity of the expression product and shows the relationship between biological activity and structure. The gene segments on mature peptide part of MAF-1 were cloned, based on the primers designed according to
Jian Liu+5 more
openaire +3 more sources
Genetic Structures and Conditions of their Expression, which Allow Receiving Native Recombinant Proteins with High Output [PDF]
We investigated the possibility of obtaining native recombinant amyloidogenic proteins by creating genetic constructs encoding fusion proteins of target proteins with Super Folder Green Fluorescent Protein (sfGFP).
Michael M. Shavlovsky, PhD, ScD¹+6 more
doaj
Automated production of recombinant human proteins as resource for proteome research
Background An arbitrary set of 96 human proteins was selected and tested to set-up a fully automated protein production strategy, covering all steps from DNA preparation to protein purification and analysis.
Poustka Annemarie+4 more
doaj +1 more source
Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for Mammalian Protein Expression Systems. [PDF]
Recombinant proteins are commonly expressed in eukaryotic expression systems to ensure the formation of disulfide bridges and proper glycosylation. Although many proteins can be expressed easily, some proteins, sub-domains, and mutant protein versions ...
Raphael Reuten+7 more
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To determine the specificity of autoantibodies for various antigenic sites on a self-protein molecule, sera from 19 patients with anti-La antibodies were tested for their reactivity with molecularly cloned La protein fragments.
E. S. St Clair+3 more
semanticscholar +1 more source
ACCUMULATION OF RECOMBINANT FUSION PROTEIN – SECRETORY ANALOG OF Ag85B AND ESAT6 MYCOBACTERIUM TUBERCULOSIS PROTEINS – IN TRANSGENIC Lemna minor L. PLANTS [PDF]
Determination of the presence of the recombinant fusion protein (ESAT6-Ag85B(ΔTMD)-6His) and its accumulation level in duckweed plants (Lemna minor L.) was the aim of the research.
A.A.Peterson+3 more
doaj +1 more source
An alternative vaccine design approach and diagnostic kits are highly required against the anticipated pandemicity caused by the South African Territories type 2 (SAT2) Foot and Mouth Disease Virus (FMDV).
Guoxiu Li+12 more
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A histidine (His)-tag is composed of six His residues and typically exerts little influence on the structure and solubility of expressed recombinant fusion proteins.
He Shuzhen+10 more
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