Results 51 to 60 of about 577,747 (248)

Design and Production of Arginine Deiminase-Azurin Recombinant Fusion Protein from Pseudomonas aeruginosa and its Confirmation by Western Blot

Novelty in Biomedicine, 2020
Background: Pseudomonas aeruginosa is a common Gram-negative, rod-shaped bacterium that has a unique genome that allowed the bacteria to produce various enzymes and proteins.
Mina Hadi, Mona Ghazi, Parvaneh Saffarian, Bahareh Hajikhani   +3 more
doaj   +1 more source

Fusing the 3’UTR of seed storage protein genes leads to massive recombinant protein accumulation in seeds

Scientific Reports, 2023
The demand for recombinant proteins is rising dramatically, and effective production systems are currently being developed. The production of recombinant proteins in plants is a promising approach due to its low cost and low risk of contamination of the ...
Masatake Kanai, Masaya Sugiyama, Maki Kondo, Kenji Yamada, Mikio Nishimura, Shoji Mano   +5 more
doaj   +1 more source

The temperature of growth and sporulation modulates the efficiency of spore-display in Bacillus subtilis

Microbial Cell Factories, 2020
Background Bacterial spores displaying heterologous antigens or enzymes have long been proposed as mucosal vaccines, functionalized probiotics or biocatalysts.
Claudia Petrillo, Stefany Castaldi, Mariamichela Lanzilli, Anella Saggese, Giuliana Donadio, Loredana Baccigalupi, Ezio Ricca, Rachele Isticato   +7 more
doaj   +1 more source

Antibody specificities of Thai and Australian scleroderma sera with topoisomerase I recombinant fusion proteins.

Journal of Immunology, 1993
Autoantibodies that react with the nuclear enzyme topoisomerase I (Topo I) are used as a diagnostic marker of diffuse scleroderma. To better define immune reactivity to Topo I, antibody epitopes in two patient populations were analyzed using recombinant ...
D. Cram, N. Fisicaro, L. Mcneilage, R. Coppel, L. Harrison   +4 more
semanticscholar   +1 more source

DEVELOPMENT OF FUSION RECOMBINANT PROTEINS BASED ON VP6 AND VP8 OF HUMAN ROTAVIRUS A

Инфекция и иммунитет, 2014
Rotaviruses are the most frequent cause of children enteritis. This infection often leads to severe dehydration of organism. Many infectious diseases are accompanied by fluid loss being the most common cause of death.
I. V. Dukhovlinov, E. G. Bogomolova, E. A. Fedorova, A. S. Simbirtsev   +3 more
doaj   +1 more source

Dissection of an old protein reveals a novel application: domain D of Staphylococcus aureus Protein A (sSpAD) as a secretion - tag

Microbial Cell Factories, 2010
Background Escherichia coli as a frequently utilized host organism for recombinant protein production offers different cellular locations with distinct qualities.
Paal Michael, Heel Thomas, Schneider Rainer, Auer Bernhard   +3 more
doaj   +1 more source

Efficient expression of fusion human epidermal growth factor in tobacco chloroplasts

BMC Biotechnology, 2023
Background Chloroplast transformation is a robust technology for the expression of recombinant proteins. Various types of pharmaceutical proteins including growth factors have been reported in chloroplasts via chloroplast transformation approach at high ...
Yunpeng Wang, Jieying Fan, Zhengyi Wei, Shaochen Xing   +3 more
doaj   +1 more source

Identification of functional regions on the tail of Acanthamoeba myosin- II using recombinant fusion proteins. II. Assembly properties of tails with NH2- and COOH-terminal deletions

Journal of Cell Biology, 1990
We used purified fusion proteins containing parts of the Acanthamoeba myosin-II tail to localize those regions of the tail responsible for each of the three steps in the successive dimerization mechanism (Sinard, J. H., W. F. Stafford, and T. D. Pollard.
John H. Sinard, David L. Rimm, T. D. Pollard   +2 more
semanticscholar   +1 more source

Recombinant production of a peroxidase-protein G fusion protein in Pichia pastoris

Journal of Biotechnology, 2016
Streptococcal protein G (SpG) binds immunoglobulin G from a broad range of mammalian species with high affinity. Chemical conjugations of SpG to the reporter enzyme horseradish peroxidase (HRP) are commonly used in immunohistochemical applications. However, commercial HRP preparations are typically isolated from horseradish roots as varying mixtures of
Ruth Birner-Gruenberger, Anton Glieder, Florian W. Krainer, Barbara Darnhofer   +3 more
openaire   +3 more sources

Human αB-crystallin as fusion protein and molecular chaperone increases the expression and folding efficiency of recombinant insulin. [PDF]

PLoS ONE, 2018
Low expression and instability are significant challenges in the recombinant production of therapeutic peptides. The current study introduces a novel expression and purification system for human insulin production using the molecular chaperone αB ...
Mohsen Akbarian, Reza Yousefi
doaj   +1 more source