Results 61 to 70 of about 577,747 (248)

The main protease 3CLpro of the SARS-CoV-2 virus: how to turn an enemy into a helper

Frontiers in Bioengineering and Biotechnology, 2023
Despite the long history of use and the knowledge of the genetics and biochemistry of E. coli, problems are still possible in obtaining a soluble form of recombinant proteins in this system. Although, soluble protein can be obtained both in the cytoplasm
Svetlana V. Belenkaya, Svetlana V. Belenkaya, Svetlana V. Belenkaya, Iuliia A. Merkuleva, Olga I. Yarovaya, Olga I. Yarovaya, Varvara Yu. Chirkova, Elena A. Sharlaeva, Daniil V. Shanshin, Ekaterina A. Volosnikova, Sergey Z. Vatsadze, Mikhail V. Khvostov, Nariman F. Salakhutdinov, Dmitriy N. Shcherbakov, Dmitriy N. Shcherbakov, Dmitriy N. Shcherbakov   +15 more
doaj   +1 more source

FimH-based display of functional eukaryotic proteins on bacteria surfaces

Scientific Reports, 2019
The demand for recombinant proteins for analytic and therapeutic purposes is increasing; however, most currently used bacterial production systems accumulate the recombinant proteins in the intracellular space, which requires denaturating procedures for ...
Markus Chmielewski, Johannes Kuehle, Danuta Chrobok, Nicole Riet, Michael Hallek, Hinrich Abken   +5 more
doaj   +1 more source

Localization of the immunologic activity in the superantigen Staphylococcal enterotoxin B using truncated recombinant fusion proteins.

Journal of Immunology, 1992
The exotoxins of certain strains of Staphylococcus aureus strains are able both to stimulate potent proliferation and induce anergy in T lymphocytes expressing the appropriate T cell Ag receptor V beta gene elements.
R. Buelow, Robyn E O'Hehir, R. Schreifels, Tammy J. Kummerehl, G. Riley, J. Lamb   +5 more
semanticscholar   +1 more source

Expression in Bacteria of the Gene Encoding the gp43 Antigen of Paracoccidioides brasiliensis: Immunological Reactivity of the Recombinant Fusion Proteins

Clinical and Vaccine Immunology, 2002
gp43 is the major diagnostic antigen of Paracoccidioides brasiliensis, the agent of paracoccidioidomycosis (PCM) in humans. In the present study, cDNA of the gp43 gene (PbGP43) was obtained by reverse transcriptase PCR, inserted into a pGEX vector in ...
S. Diniz, K. C. Carvalho, P. Cisalpino, J. Silveira, L. Travassos, R. Puccia   +5 more
semanticscholar   +1 more source

An Efficient Genome-Wide Fusion Partner Screening System for Secretion of Recombinant Proteins in Yeast

Scientific Reports, 2015
To produce rarely secreted recombinant proteins in the yeast Saccharomyces cerevisiae, we developed a novel genome-wide optimal translational fusion partner (TFP) screening system that involves recruitment of an optimal secretion signal and fusion ...
Jung-Hoon Bae, Bong Hyun Sung, Hyun-jin Kim, Soon‐Ho Park, Kwang-Mook Lim, Mi-Jin Kim, Cho-Ryong Lee, J. Sohn   +7 more
semanticscholar   +1 more source

Expression of an active form of recombinant Ty1 reverse transcriptase in Escherichia coli: a fusion protein containing the C-terminal region of the Ty1 integrase linked to the reverse transcriptase–RNase H domain exhibits polymerase and RNase H activities [PDF]

, 2000
M.L. Wilhelm, Mansour Boutabout, François‐Xavier Wilhelm   +2 more
openalex   +2 more sources

Turn TRAIL Into Better Anticancer Therapeutic Through TRAIL Fusion Proteins

Cancer Medicine
Background TNF‐related apoptosis‐inducing ligand (TRAIL) belongs to the tumor necrosis factor superfamily. TRAIL selectively induces apoptosis in tumor cells while sparing normal cells, which makes it an attractive candidate for cancer therapy ...
Yan Wang, Xin Qian, Yubo Wang, Caiyuan Yu, Li Feng, Xiaoyan Zheng, Yaya Wang, Qiuhong Gong   +7 more
doaj   +1 more source

Recombinant Glutathione S-Transferase/CD36 Fusion Proteins Define an Oxidized Low Density Lipoprotein-binding Domain*

Journal of Biological Chemistry, 1998
CD36 is a multifunctional cell-surface receptor that binds adhesion molecules such as thrombospondin-1 and collagen and modified lipids and/or lipoproteins.
Frieda A. Pearce, P. Roy, A. C. Nicholson, D. Hajjar, M. Febbraio, R. Silverstein   +5 more
semanticscholar   +1 more source

Contribution of defined amino acid residues to the immunogenicity of recombinantEscherichia coliheat-stable enterotoxin fusion proteins [PDF]

, 2000
Isabelle Batisson, Maurice Der Vartanian
openalex   +2 more sources

A fusion protein containing a lepidopteran-specific toxin from the South Indian red scorpion (Mesobuthus tamulus) and snowdrop lectin shows oral toxicity to target insects

BMC Biotechnology, 2006
Background Despite evidence suggesting a role in plant defence, the use of plant lectins in crop protection has been hindered by their low and species-specific insecticidal activity.
Fitches Elaine, Trung Nghia, Gatehouse John A   +2 more
doaj   +1 more source