Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics
FEBS Open Bio, EarlyView.Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini +2 more
wiley +1 more source
Lumbar Interbody Fusion using Low-dose of Recombinant Human Bone Morphogenetic Protein-2 (rh-BMP2); Minimum 1-year Follow-up Results at A Single Institute [PDF]
, 2021 Sang-Soo Choi +3 more
openalex +1 more source
Glycoform Modification of Secreted Recombinant Glycoproteins through Kifunensine Addition during Transient Vacuum Agroinfiltration. [PDF]
, 2018 Kifunensine, a potent and selective inhibitor of class I α-mannosidases, prevents α-mannosidases I from trimming mannose residues on glycoproteins, thus resulting in oligomannose-type glycans.
Kailemia, Muchena J +5 more
core +3 more sources
FEBS Open Bio, EarlyView.CDT1 is an essential protein for DNA replication licensing that loads the MCM complex, the eukaryotic replicative DNA helicase, onto replication origins. Overexpression of CDT1 induces cell cycle arrest at the S phase. Here we showed CDT1 inhibits the progression of replication forks by interacting with the MCM complex, leading to the stalling and ...
Takashi Tsuyama +7 more
wiley +1 more source
FimH-based display of functional eukaryotic proteins on bacteria surfaces
Scientific Reports, 2019 The demand for recombinant proteins for analytic and therapeutic purposes is increasing; however, most currently used bacterial production systems accumulate the recombinant proteins in the intracellular space, which requires denaturating procedures for ...
Markus Chmielewski +5 more
doaj +1 more source
Human αB-crystallin as fusion protein and molecular chaperone increases the expression and folding efficiency of recombinant insulin. [PDF]
PLoS ONE, 2018 Low expression and instability are significant challenges in the recombinant production of therapeutic peptides. The current study introduces a novel expression and purification system for human insulin production using the molecular chaperone αB ...
Mohsen Akbarian, Reza Yousefi
doaj +1 more source
Evaluation of a novel Escherichia coli fusion system for overproduction of recombinant immunogenic proteins [PDF]
, 2011 Recombinant protein production has been widely applied for therapeutic and diagnostic applications, namely for polyclonal antibody production. Antibodies are usually raised against a specific protein by immunization of animals with the purified protein.
Almeida, André +6 more
core
Passaging of a Newcastle disease virus pigeon variant in chickens results in selection of viruses with mutations in the polymerase complex enhancing virus replication and virulence [PDF]
, 2010 Some Newcastle disease virus (NDV) variants isolated from pigeons (pigeon paramyxovirus type 1; PPMV-1) do not show their full virulence potential for domestic chickens but may become virulent upon spread in these animals.
Dortmans, J.C.F.M. +3 more
core +2 more sources
A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms
FEBS Open Bio, EarlyView.We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley +1 more source
Design of an in vivo cleavable disulfide linker in recombinant fusion proteins
BioTechniques, 2010 In order to achieve optimal biological activity and desired pharmacokinetic profiles, a dithiocyclopeptide linker was designed for in vivo release of protein domains from a recombinant fusion protein.
Xiaoying Chen +3 more
doaj +1 more source