Results 131 to 140 of about 4,645,981 (305)
Expression and purification of recombinant human coagulation Factor VII fused to His-Tag through Gateway technology [PDF]
Background. Factor VII is a plasma glycoprotein that participates in the coagulation process leading to generation of fibrin. Construction, expression and purification of recombinant FVII fused to poly histidin tag through gateway technology ...
عدالتی فتح آباد, مهدی +6 more
core +1 more source
Molecular Oncology, EarlyView.RETRACTION: P. Merikhian, B. Darvishi, N. Jalili, M. R. Esmailinejad, A. S. Khatibi, S. M. Kalbolandi, M. Salehi, M. Mosayebzadeh, M. S. Barough, K. Majidzadeh‐A, F. Yadegari, F. Rahbarizadeh, and L. Farahmand, “Recombinant Nanobody Against Muc1 Tandem Repeats Inhibits Growth, Invasion, Metastasis, and Vascularization of Spontaneous Mouse Mammary ...
wiley +1 more source
IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo
FEBS Open Bio, EarlyView.To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley +1 more source
BMC CancerBackground Many long noncoding RNAs (lncRNAs) with altered expression significantly influence colorectal cancer (CRC) progression and behavior. The functions of many lncRNAs in CRC are not clear yet. This study aimed to discover novel lncRNA entities and
Xinzhi Miao +4 more
doaj +1 more source
FEBS Open Bio, EarlyView.Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada +7 more
wiley +1 more source
FEBS Open Bio, EarlyView.TGF‐β1 stimulation downregulates lysosomal channel TRPML1 in pulmonary fibroblasts. The TRPML1 agonist ML‐SA5 suppressed fibroblast‐to‐myofibroblast activation and collagen production. Mechanistically, ML‐SA5 inhibited mTOR phosphorylation, restored autophagic flux, and its effects were enhanced by rapamycin (mTOR inhibitor) and reversed by MHY1485 ...
Jiatong Yao +10 more
wiley +1 more source
Iranian Journal of Medical Sciences, 2014 Background:Amylin and Salmon Calcitonin belong to the calcitonin family of peptides and have high affinity binding sites in the rat spinal cord. The aim of this study was to characterize receptors for Amylin and Salmon Calcitonin functionally in the ...
Zahra Khoshdel +2 more
doaj
FEBS Open Bio, EarlyView.During the current opioid crisis, the majority of overdose deaths are attributed to ultra‐potent synthetic opioids, such as fentanyl. While fentanyl exerts its action via opioid receptors, we propose that potassium channels in the brain blood vessels may also sense fentanyl.
Shiwani Thapa +4 more
wiley +1 more source
Sex significantly influences transduction of murine liver by recombinant adeno-associated viral vectors through an androgen-dependent pathway. [PDF]
, 2003 A systematic evaluation of the influence of sex on transduction by recombinant adeno-associated viral vector (rAAV) indicated that transgene expression after liver-targeted delivery of vector particles was between 5- to 13-fold higher in male mice ...
Spence, Y +4 more
core
Characterization of the key membrane protein cytochrome Cyc2 from Ghiorsea bivora
FEBS Open Bio, EarlyView.This study reports the first detailed biochemical analysis of Cyc2TAG1_1, a cytochrome protein involved in microbial iron oxidation. We produced and purified this enzyme in E. coli, then measured its iron‐oxidizing efficiency, and characterized its electrical and structural properties.
Josiane Azizi +6 more
wiley +1 more source