Results 71 to 80 of about 1,320,877 (307)

Leishmania tarentolae: taxonomic classification and its application as a promising biotechnological expression host

, 2019
In this review, we summarize the current knowledge concerning the eukaryotic protozoan parasite Leishmania tarentolae, with a main focus on its potential for biotechnological applications.
Klatt, S., Konthur, Z., Maslov, D., Simpson, L.   +3 more
core   +1 more source

CD47 promotes mitogen‐activated protein kinase and epithelial‐to‐mesenchymal transition molecular programs to drive prometastatic phenotypes in non‐small cell lung cancer

Molecular Oncology, EarlyView.
Beyond its role in immune evasion, this study identified that CD47 drives tumor‐intrinsic signaling in non‐small cell lung cancer (NSCLC). Transcriptomic profiling and functional studies revealed that CD47 regulates cell adhesion, migration, and metastasis through an ERK–EMT signaling axis.
Asa P.Y. Lau, Lilian G. Zhai, Ryunosuke Hoshi, Zackary Rousseau, Abraam Zakhary, Yin Fang Wu, Rola M. Saleeb, Heyu Ni, Kelsie L. Thu   +8 more
wiley   +1 more source

Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris

BMC Biotechnology, 2019
Background The microbial transglutaminase (MTG) is inactive when only the mature sequence is expressed in Pichia pastoris. Although co-expression of MTG and its N-terminal pro-peptide can obtain the active MTG, the enzyme activity was still low.
Xiaoping Song, Changsheng Shao, Yugang Guo, Yajie Wang, Jingjing Cai   +4 more
doaj   +1 more source

Lipid-free Antigen B subunits from echinococcus granulosus: oligomerization, ligand binding, and membrane interaction properties [PDF]

, 2015
Background: The hydatid disease parasite Echinococcus granulosus has a restricted lipid metabolism, and needs to harvest essential lipids from the host.
Córsico, Betina, Ferreira, Ana M., Franchini, Gisela R., Kennedy, Malcolm W., Pórfido, Jorge L., Silva-Álvarez, Valeria   +5 more
core   +5 more sources

Overview of the Purification of Recombinant Proteins [PDF]

Current Protocols in Protein Science, 2015
AbstractWhen the first version of this unit was written in 1995, protein purification of recombinant proteins was based on a variety of standard chromatographic methods and approaches, many of which were described and mentioned throughoutCurrent Protocols in Protein Science. In the interim, there has been a shift toward an almost universal usage of the
openaire   +2 more sources

Heterozygous loss‐of‐function alleles associate the conserved 3′‐5′ exoribonuclease EXOSC10 with hypersensitivity to the anticancer drug 5‐fluorouracil

Molecular Oncology, EarlyView.
EXOSC10, an essential nuclear RNA exosome‐associated 3′‐5′ exoribonuclease, is inhibited by the anticancer drug 5‐fluorouracil (5‐FU), and EXOSC10 depletion increases 5‐FU sensitivity. The colon‐cancer variant EXOSC10S402T, located in a proteolysis motif, is stable and nuclear but nonfunctional in vivo.
Radhika Sain, Yann Le Page, Frédéric Percevault, Emmanuelle Becker, Luc Negroni, Almudena Fernandez, Marta Cantero, Diego Muñoz‐Santos, Lluís Montoliu, Cyrille Garnier, Michael Primig   +10 more
wiley   +1 more source

Human endogenous retrovirus np9 gene is over expressed in chronic lymphocytic leukemia patients

Leukemia Research Reports, 2014
The human genome contains a large number of endogenous retroviruses (HERVs). Their reactivation has frequently been observed in patients with cancer.
Sabrina Fischer, Natalia Echeverría, Gonzalo Moratorio, Ana Inés Landoni, Guillermo Dighiero, Juan Cristina, Pablo Oppezzo, Pilar Moreno   +7 more
doaj   +1 more source

Recombinant subunit vaccines against Neospora caninum [PDF]

, 2007
The intracellular protozoan parasite Neospora caninum is an important cause of bovine abortion and congenital infection in many countries. Currently there is no effective control method available, and development of a vaccine has been suggested as a ...
Pinitkiatisakul, Sunan
core  

Horseradish and soybean peroxidases: comparable tools for alternative niches? [PDF]

, 2006
Horseradish and soybean peroxidases (HRP and SBP, respectively) are useful biotechnological tools. HRP is often termed the classical plant heme peroxidase and although it has been studied for decades, our understanding has deepened since its cloning and ...
Alonso Lomillo, Alpeeva, Azevedo, Azevedo, Azevedo, Barry J. Ryan, Bruns, Bódalo, Campanella, Castilho, Castillo, Chen, Cho, Ciarán Ó’Fágáin, Colas, Dachs, Dai, Dai, dos Santos, Dotsikas, Dunford, Duroux, Ferapontova, Ferapontova, Ferapontova, Folkes, Gajhede, Gazaryan, Gazaryan, Gijzen, Gray, Gustafsson, Henriksen, Hoffmann, Howes, Howes, Howes, Hoyle, Hushpulian, Ignatenko, Jung, Kamal, Kamiya, Katz, Kazlauskas, Kenausis, Kim, Kim, Klibanov, Knutson, Konash, Kreiner, Lee, Lei, Li, Li, Lin, Lin, Lindgren, Liu, Luong, Mendive, Michizoe, Morawski, Morawski, Morgan, Morimoto, Mukai, Nagano, Neil Carolan, Newmyer, Newmyer, Nicell, Ozaki, Patel, Paul, Penalva, Poljaková, Rahman, Rauf, Rodriguez-Lopez, Rossiter, Sakharov, Schmitz, Schuvailo, Shipovskov, Smith, Smith, Song, Sun, Tafazoli, Tanaka, Teilum, Tupper, Tønning, van de Velde, van de Velde, Varnado, Veitch, Veitch, Veitch, Veitch, Verpoorte, Vreeke, Wandersman, Wang, Wang, Wanger, Welinder, Welinder, Welinder, Won, Woodard, Wu, Wuhrer, Yan, Yuan, Zhang, Zhang, Zhu   +119 more
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Interaction of HS1BP3 with cortactin modulates TKS5 localisation, cell secretion and cancer malignancy

Molecular Oncology, EarlyView.
Here, we demonstrate that HS1BP3 interacts with Cortactin through a proline‐rich region (PRR3.1) and show that this interaction, and HS1BP3 itself, promote cancer cell proliferation and invasion. Inhibition of this interaction leads to build‐up of TKS5 in multivesicular endosomes and altered secretion of CD63 and CD9, providing an explanation for the ...
Arja Arnesen Løchen, Kristiane Søreng, Chiara Veroni, Laura Trachsel‐Moncho, Nagham Asp, Robin Gaupset, Lars Gustav Lyckander, Helene Knævelsrud, Lars Eftang, Anne Simonsen   +9 more
wiley   +1 more source