Results 71 to 80 of about 1,309,435 (302)

Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris

BMC Biotechnology, 2019
Background The microbial transglutaminase (MTG) is inactive when only the mature sequence is expressed in Pichia pastoris. Although co-expression of MTG and its N-terminal pro-peptide can obtain the active MTG, the enzyme activity was still low.
Xiaoping Song, Changsheng Shao, Yugang Guo, Yajie Wang, Jingjing Cai   +4 more
doaj   +1 more source

Recombinant subunit vaccines against Neospora caninum [PDF]

, 2007
The intracellular protozoan parasite Neospora caninum is an important cause of bovine abortion and congenital infection in many countries. Currently there is no effective control method available, and development of a vaccine has been suggested as a ...
Pinitkiatisakul, Sunan
core  

Complexity of the Ruminococcus flavefaciens FD-1 cellulosome reflects an expansion of family-related protein-protein interactions [PDF]

, 2017
This work was supported in part by the European Union, Area NMP.2013.1.1–2: Self-assembly of naturally occurring nanosystems: CellulosomePlus Project number: 604530, and by the EU Seventh Framework Programme (FP7 2007–2013) under the WallTraC project ...
Alves, Victor D, Barak, Yoav, Bayer, Edward A, Borovok, Ilya, Bule, Pedro, Cardoso, Vânia, Dassa, Bareket, Flint, Harry J, Fontes, Carlos M G A, Gilbert, Harry J, Hamberg, Yuval, Israeli-Ruimy, Vered, Jindou, Sadanari, Lamed, Raphael, Moraïs, Sarah, Najmudin, Shabir, Slutzki, Michal, White, Bryan A   +17 more
core   +1 more source

Interaction of HS1BP3 with cortactin modulates TKS5 localisation, cell secretion and cancer malignancy

Molecular Oncology, EarlyView.
Here, we demonstrate that HS1BP3 interacts with Cortactin through a proline‐rich region (PRR3.1) and show that this interaction, and HS1BP3 itself, promote cancer cell proliferation and invasion. Inhibition of this interaction leads to build‐up of TKS5 in multivesicular endosomes and altered secretion of CD63 and CD9, providing an explanation for the ...
Arja Arnesen Løchen, Kristiane Søreng, Chiara Veroni, Laura Trachsel‐Moncho, Nagham Asp, Robin Gaupset, Lars Gustav Lyckander, Helene Knævelsrud, Lars Eftang, Anne Simonsen   +9 more
wiley   +1 more source

Human endogenous retrovirus np9 gene is over expressed in chronic lymphocytic leukemia patients

Leukemia Research Reports, 2014
The human genome contains a large number of endogenous retroviruses (HERVs). Their reactivation has frequently been observed in patients with cancer.
Sabrina Fischer, Natalia Echeverría, Gonzalo Moratorio, Ana Inés Landoni, Guillermo Dighiero, Juan Cristina, Pablo Oppezzo, Pilar Moreno   +7 more
doaj   +1 more source

Physiology-based IVIVE predictions of tramadol from in vitro metabolism data [PDF]

, 2014
To predict the tramadol in vivo pharmacokinetics in adults by using in vitro metabolism data and an in vitro-in vivo extrapolation (IVIVE)-linked physiologically-based pharmacokinetic (PBPK) modeling and simulation approach (SimcypA (R)).
Allegaert, Karel, Annaert, Pieter, Boussery, Koen, Colin, Pieter, Cuyckens, Filip, Mannens, Geert, Snoeys, Jan, T'jollyn, Huybrecht, Van Bocxlaer, Jan, Van Peer, Achiel, Vermeulen, An   +10 more
core   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Controlling sap-sucking insect pests with recombinant endophytes expressing plant lectin [PDF]

, 2010
We developed a novel pest management strategy, which uses endophytes to express anti-pest plant lectins. Fungal endophyte of Chaetomium globosum YY-11 with anti-fungi activities was isolated from rape seedlings, and bacterial endophytes of SJ-10 ...
Gaofu Qi, Nan Lan, Xianfang Zhang, Xinrong Ma, Xiuyun Zhao   +4 more
core   +1 more source

Production and characterization of monoclonal antibodies raised against recombinant human granzymes A and B and showing cross reactions with the natural proteins [PDF]

, 1993
The human serine proteases granzymes A and B are expressed in cytotoplasmic granules of activated cytotoxic T lymphocytes and natural killer cells. Recombinant granzyme A and granzyme B proteins were produced in bacteria, purified and then used to raise ...
Borst, Jannie, Brakenhoff, Just P.J., Hack, C. Erik, Kamp, Angela M., Kummer, J. Alain, Radosevic, K., Radosevic, Katarina, van Katwijk, Marcel, van Leeuwen, Anne Marie, Verweij, Cornelis L.   +9 more
core   +4 more sources

Domain associated with zinc fingers‐containing NF90‐NF45 complex inhibits m6A modification of primary microRNA by suppressing METTL3/14 activity

FEBS Open Bio, EarlyView.
NF90–NF45 functions as a negative regulator of methyltransferase‐like 3/14 (METTL3/14)‐mediated N6‐methyladenosine (m6A) modification on primary microRNAs (pri‐miRNAs). NF90–NF45 binds to anti‐oncogenic pri‐miRNAs and inhibits their m6A modification, thereby suppressing the biogenesis of anti‐oncogenic miRNAs.
Takuma Higuchi, Shunsuke Morioka, Keiko Morisawa, Kazutsugu Matsukawa, Shingo Ashida, Takeshi Suzuki, Shuji Sakamoto   +6 more
wiley   +1 more source