Results 91 to 100 of about 4,645,981 (305)

Biological and genomic characterization of Escherichia phage KBU_SV1 active against carbapenem-resistant Escherichia coli

Frontiers in Microbiology
Carbapenem-resistant Escherichia coli infections are increasingly difficult to treat, and bacteriophages have been highlighted as a promising tool to control such multidrug-resistant bacteria. Here, we isolated and characterized Escherichia phage KBU_SV1
Sevil Öztaş, Abdulkerim Karaynir, Rumeysa Gulsu Ozkan, Bulent Bozdogan, Devrim Dündar   +4 more
doaj   +1 more source

Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation

Molecular Oncology, EarlyView.
How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim, Alvin Kunyao Guo, Yoko Itahana, Srinivasaraghavan Kannan, Valerie Goh, Mingmin Ong, Terina Yan Wen Tay, Weiying Ye, Sabhashina Radha Krishnan, Jun Nishiyama, Chandra S Verma, Koji Itahana   +11 more
wiley   +1 more source

Engineered Recombinant Protein Production Systems Originated from Escherichia coli [PDF]

, 2019
Selection of a suitable host strain is an important step in recombinant production of heterologous proteins. As the prokaryotic systems and especially Escherichia coli (E.
Ranjbari, Javad
core   +1 more source

Expression of the murine cytomegalovirus glycoprotein H by recombinant vaccinia virus [PDF]

, 1994
The sequence of the gene encoding glycoprotein H (gH) of murine cytomegalovirus (MCMV) strain Smith was determined and compared with the sequence of the gH of MCMV strain K181. Transcriptional analysis showed that gH is encoded by a large mRNA of 5.0 kb,
Rapp, Maria, Koszinowski, Ulrich H., Lucin, Pero, Messerle, Martin, Loh, Lambert C.   +4 more
core   +1 more source

Targeting the EpCAM‐AXL axis to overcome drug resistance in lung cancer

Molecular Oncology, EarlyView.
Lung cancer cells often evade therapy by hijacking signaling pathways. We reveal that cleaved EpCAM (sEpCAM) stabilizes the oncogenic protein AXL, driving NF‐κB and STAT3‐mediated chemoresistance. This EpCAM‐AXL axis identifies a high‐risk patient subset with poor prognosis.
Alexa Guerrero‐Alba, Mark Shacker, Timothy P. Fleming, Michael A. Smith, Ross M. Bremner, Narendra V. Sankpal   +5 more
wiley   +1 more source

Recombinant protein characterization.

, 2019
Recombinant protein characterization.
Nykola C. Jones (2863304), Søren V. Hoffmann (2067904), Maximilian W. A. Skoda (1762540), Ulrich Bergmann (115067), Hanna Wacklin-Knecht (6812279), Erik I. Hallin (6812276), Cecilie K. Kristiansen (6812273), Oda C. Krokengen (6812267), Arne Raasakka (180418), Salla Ruskamo (3861415), Guro H. Vatne (6812270), Robert Barker (1558021), Petri Kursula (90066)   +12 more
core   +1 more source

Cross-kingdom regulation of plant microRNAs: potential application in crop improvement and human disease therapeutics

Frontiers in Plant Science
Plant microRNAs (miRNAs) are small non-coding RNA molecules that usually negatively regulate gene expression at the post-transcriptional level. Recent data reveal that plant miRNAs are not limited to individual plants but can transfer across different ...
Lei Shi, Lei Shi, Chao Guo, Miaomiao Fang, Yingmei Yang, Fei Yin, Yuan Shen, Yuan Shen   +7 more
doaj   +1 more source

Characterization of therapeutic antibodies targeting lymphatic and vascular resistance factor for the treatment of hypervascularized and highly metastatic cancers

Molecular Oncology, EarlyView.
LVRF (lymphatic and vascular resistance factor) is a VEGFA splice variant upregulated in response to anti‐angiogenic therapy that fosters tumor adaptation through enhanced vascularization, lymphangiogenesis, and metastatic dissemination. Targeting LVRF with fully human neutralizing antibodies disrupts these processes and limits resistant tumor growth ...
Marion David, Arthur Karaulic, Audrey Bennetot, Clémence Fournier, Quentin Odet, Arthur Gouraud, Isabelle Bourget, Cédric Gaggioli, Sandy Giuliano, Frédéric Luciano, Olivier Léger, Zaki Sellam, Gilles Pagès   +12 more
wiley   +1 more source

Silver inhibition on recombinant flavin reductase from Citrobacter freundii A1 [PDF]

, 2012
In this study, the flavin reductase (Fre) of Citrobatter freundii A1 was expressed in E. coli DH5a host with 1 mM IPTG induction. This recombinant protein was fused to 6xHis-tag, thus promising easier IMAC purification of the expressed enzyme.
Mamat, Nor Sasha Izana
core  

EXPRESSION AND CHARACTERIZATION OF RECOMBINANT LEPTOSPIRAL OUTER MEMBRANE PROTEIN LIPL32 FROM LEPTOSPIRA INTERROGANS SEROVAR AUTUMNALIS [PDF]

, 2020
. Leptospira interrogans serovar autumnalis, a causative agent of leptospirosis in Thailand, was isolated from a patient for DNA extraction and amplification of LipL32 gene by polymerase chain reaction (PCR).
Interrogans, Autumnalis, From L Recombinant Lipl32   +2 more
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