Results 121 to 130 of about 797,107 (316)

Recombinant Proteins: Hopes for Tissue Engineering

BioImpacts, 2012
Proteins constitute a group of key molecules with many applications in tissue engineering. Use of proteins provided from natural sources has several limitations which are overcome by the use of recombinant proteins.
Sepideh Hamzehlou, Mohammad Morad Farajollahi, Ahmad Mehdipour, Ali Samadikuchaksaraei   +3 more
doaj   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Production of Recombinant Lectins from Garlic (Allium Sativum) Bulbs and Their Insecticidal Activity Against Hemipteran Insects. [PDF]

, 2008
Lectins have long been established as potential agents for use in control of insect pest species. In this study the use of garlic bulb lectins against phloem-feeding hemipteran insect pests is investigated.
Wiles, Duncan Peter
core  

Optimization of a novel expression system for recombinant protein production in CHO cells

Scientific Reports
Chinese hamster ovary (CHO) cells are common mammalian cell lines for expressing recombinant proteins, yet the expression level of recombinant proteins is still hindered.
Junhe Zhang, Chenyang Du, Yue Pan, Zhan Zhang, Ruoyuan Feng, Mengyao Ma, Tianyun Wang   +6 more
doaj   +1 more source

A yeast model of 5‐oxoproline accumulation reveals a general toleration to 5‐oxoproline

FEBS Open Bio, EarlyView.
Using a yeast model, we show that even high accumulation of 5‐oxoproline causes only mild cellular stress and does not trigger oxidative stress. Instead, cells adapt by activating efflux pumps and diverse protective pathways, suggesting that previously proposed harmful effects of 5‐oxoproline may arise from indirect metabolic imbalances rather than the
Pratiksha Dubey, Vaishnavi Sanjayan, Praveen Singh, Shantanu Sengupta, Anand Kumar Bachhawat   +4 more
wiley   +1 more source

Evaluation of a reverse tetracycline inducible system in recombinant BCG to improve stability and immunogenicity [PDF]

, 2011
The aim of this study is to utilise the tetracycline dependent gene regulation system to down-regulate recombinant antigen expression in BCG during the expansion of seed stocks and up-regulate expression prior to or post ...
Mbele, Prisca
core  

Scalable production of recombinant three-finger proteins: from inclusion bodies to high quality molecular probes

Microbial Cell Factories
Background The three-finger proteins are a collection of disulfide bond rich proteins of great biomedical interests. Scalable recombinant expression and purification of bioactive three-finger proteins is quite difficult.
Jiang Xu, Xiao Lei, Ao Li, Jun Li, Shuxing Li, Lin Chen   +5 more
doaj   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

Exploitation of the protein tubulin for controlling African trypanosomiasis [PDF]

, 2005
This thesis presents the results of an investigation into the structural protein, tubulin, as a potential target for anti-trypanosomatid drug discovery and vaccine development.
Giles, Natalie
core   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source