Complementation of recombinant baculoviruses by coinfection with wild-type virus facilitates production in insect larvae of antigenic proteins of hepatitis B virus and influenza virus. [PDF]
, 1989 Peter M. Price +4 more
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Overview of the Purification of Recombinant Proteins [PDF]
Current Protocols in Protein Science, 2015 AbstractWhen the first version of this unit was written in 1995, protein purification of recombinant proteins was based on a variety of standard chromatographic methods and approaches, many of which were described and mentioned throughoutCurrent Protocols in Protein Science. In the interim, there has been a shift toward an almost universal usage of the
openaire +3 more sources
Bioorthogonal Engineering of Cellular Microenvironments Using Isonitrile Ligations
Advanced Functional Materials, EarlyView.Highly selective chemistries are required for fabrication and post‐cross–linking modification of cell‐encapsulating hydrogels used in tissue engineering applications. Isonitrile ligation reactions represent a promising class of bioorthogonal chemistries for engineering hydrogel‐based cellular microenvironments. Isonitrile‐based hydrogels are stable and
Ping Zhou +2 more
wiley +1 more source
Microbial Cell FactoriesBackground The three-finger proteins are a collection of disulfide bond rich proteins of great biomedical interests. Scalable recombinant expression and purification of bioactive three-finger proteins is quite difficult.
Jiang Xu +5 more
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The recombinant 65-kD heat shock protein of Mycobacterium bovis Bacillus Calmette-Guerin/M. tuberculosis is a target molecule for CD4+ cytotoxic T lymphocytes that lyse human monocytes. [PDF]
, 1988 Tom H. M. Ottenhoff +4 more
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Advanced Functional Materials, EarlyView.This review highlights recent advances in engineering artificial antigen‐presenting cells (aAPCs) as alternatives to dendritic cells for T cell expansion. Key design principles inspired by the immunological synapse are discussed, with emphasis on strategies for polyclonal and antigen‐specific T cell expansion.
Nguyen Thi Nguyen, Yu Seok Youn
wiley +1 more source
Immunization of mice with dengue structural proteins and nonstructural protein NS1 expressed by baculovirus recombinant induces resistance to dengue virus encephalitis [PDF]
, 1988 Y M Zhang +7 more
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Advanced Functional Materials, EarlyView.Synthetic cells are engineered herein to respond to an external chemical messenger by the activation of intracellular catalysis. The chemical messenger molecules are catalytically generated by an extracellular enzyme or a mineral surface, whereas the intracellular catalysis emerges via direct enzyme activation or via protein refolding.
Dante G. Andersen +5 more
wiley +1 more source
Recombinant Collagenlike Proteins [PDF]
A group of collagenlike recombinant proteins containing high densities of biologically active sites has been invented. The method used to express these proteins is similar to a method of expressing recombinant procollagens and collagens described in U. S.
Fertala, Andzej
core +1 more source
High‐Throughput Microfluidic‐Mediated Assembly of Layer‐By‐Layer Nanoparticles
Advanced Functional Materials, EarlyView.The layer‐by‐layer (LbL) assembly of polymer films on nanoparticle (NP) surfaces is a promising technique for targeted drug delivery. Despite its success in preclinical mouse models, a current good manufacturing practices (cGMP) compliant, clinical‐scale production method has been lacking.
Ivan S. Pires +4 more
wiley +1 more source