Results 111 to 120 of about 520,105 (221)

Enhanced CRISPR‐cas systems for genome editing and molecular diagnostics

Interdisciplinary Medicine, EarlyView.
This review systematically summarizes the major optimization strategies for CRISPR‐Cas systems, focusing on the engineering of Cas proteins, guide RNAs, reporter probes, and the incorporation of chemical additives. The aim is to enhance editing precision and diagnostic capability, thereby expanding the CRISPR system's potential for broader biomedical ...
Sheng Li, Han Li, Bojie Chen, Ling Jiang, Keyu Long, Jiaqi Lang, Xingxiang Pu, Erhu Xiong   +7 more
wiley   +1 more source

Establishment and evaluation of qPCR and real‐time recombinase‐aided amplification assays for detection of largemouth bass ranavirus

, 2022
Largemouth bass ranavirus disease (LMBVD) caused by largemouth bass ranavirus (LMBV) has resulted in severe economic losses in the largemouth bass (Micropterus salmoides) farming industry in China.
Xianlin Zhao, Fan, Zhaobin, Yanmin Guo, Zhaobin Fan, Jin, Yuqi, Mai, Qianyi, Weiqiang Liu, Bergmann, Sven, Yahui Wang, Guo, Yanmin, Yuqi Jin, Zeng, Weiwei, Wang, Yahui, Dong, Hanxu, Sun, Dongli, Sven M. Bergmann, Liu, Weiqiang, Qianyi Mai, Dongli Sun, Weiwei Zeng, Zhao, Xianlin, Hanxu Dong   +21 more
core   +1 more source

Two-stage detection of plasmodium spp. by combination of recombinase polymerase amplification and loop-mediated isothermal amplification assay

, 2022
We developed a combination of recombinase polymerase and loop-mediated isothermal amplification methods (RAMP) for rapid screening of five human Plasmodium spp. simultaneously.
Lai, Meng Yee, Lau, Yee Ling
core   +1 more source

Rapid Detection of Klebsiella pneumoniae in Infants With Severe Infection Disease by Instant Nanofluorescent Microspheres‐Immunochromatography Combined With Recombinase Polymerase Isothermal Amplification Technology

Journal of Clinical Laboratory Analysis, EarlyView.
Schematic illustration of the RPA‐FICTS platform for rapid detection of Klebsiella pneumoniae in infants. Genomic DNA extracted from clinical samples is amplified by recombinase polymerase amplification (RPA) targeting the mdh gene at 37°C, and the biotin‐ and digoxigenin‐labeled amplicons are subsequently detected on a nanofluorescent microsphere ...
Yiping Liu, Yi Lu, Xin Wang, Hanzhong Yu, Yumeng Lu, Minghui Chen, Feifan Zhu, Xing Lv, Li Wang, Ying Chen   +9 more
wiley   +1 more source

The effect of oxidative stress on the mutation rate of Mycobacterium tuberculosis with impaired catalase/peroxidase function. [PDF]

, 2008
OBJECTIVES: To determine the effect of oxidative stress on isoniazid-resistant Mycobacterium tuberculosis deficient in catalase/peroxidase activity to varying degrees through mutation in katG.
O'Sullivan, Denise M., Gillespie, S. H., O'Sullivan, DM, T. D. McHugh, Mchugh, T. D., McHugh, TD, S. H. Gillespie, Gillespie, SH, D. M. O'Sullivan   +8 more
core   +1 more source

Low‐Concentration Enhancement Effect: Enabling Sensitive Detection of EML4‐ALK Variants via Multiplex RT‐MIRA

Journal of Clinical Laboratory Analysis, EarlyView.
This study developed an optimized multiplex RT‐MIRA assay for the simultaneous detection of EML4‐ALK variants, achieving single‐copy sensitivity. Unexpectedly, during the development of the reaction system, we discovered a “low‐concentration enhancement effect,” where reducing primer or probe concentrations within a specific range markedly improved ...
Xiao‐Dong Ren, Xi‐Yao Luo, Jin‐Mi Li, Xian‐Ge Sun, Jing Lv, Hua‐Liang Xiao, Qiu‐Shi Wang, Hong Wang, Chun‐Yan Wang, Hao‐Zheng Li, Qing Huang   +10 more
wiley   +1 more source

Anticancer potential of berberine: Molecular pathways and current clinical trial perspectives

JSFA reports, EarlyView.
Abstract Cancer is constantly rising mortality rates due to its late prognosis, poor management, and expensive treatment. Multi‐sectoral approaches for cancer management include hygienic practices, synthetic drug exploitation, radiation therapy, and diet modifications.
Muhammad Maaz, Muhammad Tauseef Sultan, Muhammad Israr, Waqas Ahmad Khan, Fahad Tanveer, Muzzamal Hussain, Muhammad Imran, Ahmed Mujtaba, Anjuman Gul Memon, Muhammad Atif, Entessar Al Jbawi   +10 more
wiley   +1 more source

Rapid detection of shrimp white spot syndrome virus by real time, isothermal recombinase polymerase amplification assay [PDF]

, 2014
White spot syndrome virus (WSSV) causes large economic losses to the shrimp aquaculture industry, and thus far there are no efficient therapeutic treatments available against this lethal virus.
Wang, Yongjie, Manfred Weidmann (448254), Yingjie Pan (590513), Manfred Weidmann, Yu, Yongxin, Pan, Yingjie, Weidmann, Manfred, Yongxin Yu (300284), Xiaoming Xia, Yingjie Pan, Yongjie Wang (152774), Yongxin Yu, Xiaoming Xia (615271), Shuling Yan (590512), Wang, Y., Shuling Yan, Yan, Shuling, Xia, Xiaoming, Yongjie Wang   +18 more
core   +1 more source

Disease control in oilseed rape: Current insights and perspectives for next‐generation integrated pest management

Pest Management Science, EarlyView.
Winter oilseed rape in Europe suffers frequent pathogen losses. This review outlines key integrated pest management (IPM) strategies, rotation, residue and tillage management, optimized sowing, resistance, and targeted fungicides supported by forecasting.
Jon S West, Małgorzata Jędryczka, Nazanin Zamani‐Noor   +2 more
wiley   +1 more source

Conditional mutagenesis in the immune system: targeting the expression of the iCre2 recombinase to neutrophils and macrophages [PDF]

, 2010
Conditional mutagenesis allows the introduction of tissue specific mutations in the mouse and is of crucial importance in converting genome sequence information into functional data for biomedical research.
Könitzer, J.D.
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