Results 111 to 120 of about 131,465 (263)

Advances in Microfluidics and Lab-on-a-Chip Technologies

, 2017
Advances in molecular biology are enabling rapid and efficient analyses for effective intervention in domains such as biology research, infectious disease management, food safety, and biodefense.
Abate, Adley, Aebersold, Ahlford, Almassian, Almoguera, Asiello, Athamanolap, Baker, Becker, Blazej, Brehm-Stecher, Burger, Business-Wire, Byrnes, Cai, Cao, Chang, Chao, Chatterjee, Chin, Chin, Coupland, Craw, Crews, Crews, Czilwik, Derveaux, DeVoe, Dharmasiri, Dodsworth, Domon, Duffy, Erlich, Farrar, Fredrickson, Fu, Gagnon, Gao, Gonzalez de Castro, Grover, Hadd, Han, Hanyoup, Hardouin, Hauck, Hayden, He, He, He, Hindson, Ho, Hsieh, Huang, Hughes, Jacobs, Jansson, Jarvius, Jayamohan, Jayamohan, Jenkins, Jha, Ji, Jin, Jin, Kalra, Karimi, Karns, Khandurina, Kim, Kim, Kim, Kim, Kim, Klein, Klostranec, Konry, Kuroda, Kwasny, Kwasny, Kühnemund, Küster, Lagally, Lapierre, Lazar, Lazar, Lee, Lee, Lee, Li, Liu, Lochovsky, Macosko, Mahmoudian, Mani, Manz, Mao, Mariella, Melin, Mery, Metzker, Mezger, Mitchell, Mitchell, Neuzil, Ng, Northen, Oblath, Oxford Nanopore, Paegel, Paegel, Paliwal, Park, Pekin, Pennisi, Perez-Toralla, Pješčić, Pješčić, Pješčić, Prakash, Priye, Priye, Quick, Ro, Romanov, Safavieh, Sainiemi, Samuel, Sanvicens, Sato, Sayad, Schoenitz, Schuler, Shamsi, Shih, Song, Srbek, Stumpf, Sun, Sundberg, Tanaka, Teh, Tehranirokh, Thompson, Thuy, Tian, Tröger, Unger, Vergauwe, Volpatti, Weisenberger, Wheeler, Whitesides, Woolley, Xie, Xu, Xue, Yetisen, Yin, Zhang, Zhang, Zhu   +161 more
core   +1 more source

Persistent Wnt/β-catenin signaling determines dorsalization of the postnatal subventricular zone and neural Stem cell specification into oligodendrocytes and glutamatergic neurons [PDF]

, 2014
In the postnatal and adult central nervous system (CNS), the subventricular zone (SVZ) of the forebrain is the main source of neural stem cells (NSCs) that generate olfactory neurons and oligodendrocytes (OLs), the myelinating cells of the CNS.
Azim, Kasum, Butt, Arthur, Cantu, Claudio, Draganova, Kalina, Fischer, Bruno, Hurtado-Chong, Anahi, Raineteau, Olivier, Sommer, Lukas, Zemke, Martina   +8 more
core   +1 more source

DNA Flap‐Mediated Control of Transcription for Programmable RNA Synthesis

Angewandte Chemie International Edition, EarlyView.
Pyrimidine‐rich ssDNA flaps on the T7 promoter inhibit T7 RNA polymerase (T7RP) transcription. Leveraging this pyrimidine bias, we developed DNAzyme‐ and MNAzyme‐mediated flap promoter induced transcription control (D‐FIT and M‐FIT) systems. These platforms enable regulated transcription and sequence‐specific detection, highlighting the programmable ...
Eun Sung Lee, Jisu Woo, Seokjoon Kim, Seok Hyeon Kim, Gun Haeng Lee, Ki Soo Park   +5 more
wiley   +1 more source

DNA detection using recombination proteins.

PLoS Biology, 2006
DNA amplification is essential to most nucleic acid testing strategies, but established techniques require sophisticated equipment or complex experimental procedures, and their uptake outside specialised laboratories has been limited. Our novel approach,
Olaf Piepenburg, Colin H Williams, Derek L Stemple, Niall A Armes   +3 more
doaj   +1 more source

Digital Isothermal Quantification of Nucleic Acids via Simultaneous Chemical Initiation of Recombinase Polymerase Amplification Reactions on SlipChip [PDF]

, 2011
In this paper, digital quantitative detection of nucleic acids was achieved at the single-molecule level by chemical initiation of over one thousand sequence-specific, nanoliter isothermal amplification reactions in parallel.
Davydova, Elena K., Du, Wenbin, Ismagilov, Rustem F., Kreutz, Jason E., Piepenburg, Olaf, Shen, Feng   +5 more
core   +2 more sources

Chimeric piggyBac transposases for genomic targeting in human cells. [PDF]

, 2012
Integrating vectors such as viruses and transposons insert transgenes semi-randomly and can potentially disrupt or deregulate genes. For these techniques to be of therapeutic value, a method for controlling the precise location of insertion is required ...
Belcaid, Mahdi, Coates, Craig J, Dang, Nong C, Maragathavally, Kommineni J, Moisyadi, Stefan, Owens, Jesse B, Segal, David J, Stoytchev, Ilko, Stoytcheva, Zoia, Urschitz, Johann   +9 more
core   +5 more sources

Rapid Detection of Measles Virus Using Reverse Transcriptase/Recombinase Polymerase Amplification Coupled with CRISPR/Cas12a and a Lateral Flow Detection: A Proof-of-Concept Study

Diagnostics
The measles virus is highly contagious, and efforts to simplify its diagnosis are essential. A reverse transcriptase/recombinase polymerase amplification assay coupled with CRISPR/Cas12a and an immunochromatographic lateral flow detection (RT-RPA-CRISPR ...
Elena Pinchon, Steven Henry, F. Leon, C. Fournier-Wirth, Vincent Foulongne, J. Cantaloube   +5 more
semanticscholar   +1 more source

Strand Invasion Based Amplification (SIBA®): a novel isothermal DNA amplification technology demonstrating high specificity and sensitivity for a single molecule of target analyte.

PLoS ONE, 2014
Isothermal nucleic acid amplification technologies offer significant advantages over polymerase chain reaction (PCR) in that they do not require thermal cycling or sophisticated laboratory equipment.
Mark J Hoser, Hannu K Mansukoski, Scott W Morrical, Kevin E Eboigbodin   +3 more
doaj   +1 more source

Microfluidic method for rapid turbidimetric detection of the DNA of Mycobacterium tuberculosis using loop-mediated isothermal amplification in capillary tubes [PDF]

, 2015
We describe a microfluidic method for rapid isothermal turbidimetric detection of the DNA of Mycobacterium tuberculosis. Loop-mediated isothermal amplification is accomplished in capillary tubes for amplifying DNA in less than 15 min, and sensitivity and
Gill, P., Rafati, A.
core  

Engineering Gram-Negative Microbial Cell Factories Using Transposon Vectors [PDF]

, 2016
The construction of microbial cell factories à la carte largely depends on specialized molecular biology and synthetic biology tools needed to reprogram bacteria for modifying their existing functions or for bestowing them with new-to-Nature tasks.
Aparicio, Tomás, Lorenzo, Víctor de, Martínez-García, Esteban, Nikel, Pablo I.   +3 more
core   +1 more source