Results 221 to 230 of about 131,465 (263)
Corrigendum to: Development of a reverse-transcription recombinase polymerase amplification assay with a lateral flow assay for rapid detection of avian orthoavulavirus. [PDF]
J Vet Diagn Investeuropepmc +1 more source
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Droplet Digital Recombinase Polymerase Amplification (Ddrpa) Reaction Unlocking Via Picoinjection
SSRN Electronic Journal, 2021Recombinase polymerase amplification (RPA) has been recognized as a promising isothermal amplification method for nucleic acid detection. However, the digital format of RPA is still challenging to implement due to its MgOAc-initiated reaction feature and the inherent non-specific amplification.
Johnson Q. Cui +6 more
openaire +3 more sources
Analytical Chemistry, 2023
Timely identification of human papillomavirus (HPV) infection is crucial for the prevention of cervical cancer. Current HPV detection methods mainly rely on polymerase chain reaction (PCR), which often requires bulky equipment and a long assay time.
Hu Zhou +10 more
semanticscholar +1 more source
Timely identification of human papillomavirus (HPV) infection is crucial for the prevention of cervical cancer. Current HPV detection methods mainly rely on polymerase chain reaction (PCR), which often requires bulky equipment and a long assay time.
Hu Zhou +10 more
semanticscholar +1 more source
Nucleic acid quantification with amplicon yield in recombinase polymerase amplification
Analytical Chemistry, 2022AbstractAmplification-based qPCR provides accurate and sensitive nucleic acid quantification. However, the requirement of temperature cycling and real-time monitoring limits its translation to different settings. Here, we adapted isothermal Recombinase Polymerase Amplification (RPA) reaction to develop a semi-quantitative method that relies on final ...
Priyanka Valloly, Rahul Roy
openaire +2 more sources
Small, 2023
Digital nucleic acid detection based on microfluidics technology can quantify the initial amount of nucleic acid in the sample with low equipment requirements and simple operations, which can be widely used in clinical and in vitro diagnosis.
Weihong Yin +6 more
semanticscholar +1 more source
Digital nucleic acid detection based on microfluidics technology can quantify the initial amount of nucleic acid in the sample with low equipment requirements and simple operations, which can be widely used in clinical and in vitro diagnosis.
Weihong Yin +6 more
semanticscholar +1 more source
Journal of Agricultural and Food Chemistry, 2023
Acute hepatopancreatic necrosis disease (AHPND) is one of the most devastating diseases in aquaculture, causing significant economic losses in seafood supplies worldwide.
Pei Wang +7 more
semanticscholar +1 more source
Acute hepatopancreatic necrosis disease (AHPND) is one of the most devastating diseases in aquaculture, causing significant economic losses in seafood supplies worldwide.
Pei Wang +7 more
semanticscholar +1 more source
ACS Sensors, 2023
Rising consumption, large-scale production, and widespread distribution have been accompanied by an increase in the number of Salmonella infections reported to implicate contaminated food products. We developed a portable origami microfluidic device that
Yunxuan Chen, Yaxi Hu, Xiaonan Lu
semanticscholar +1 more source
Rising consumption, large-scale production, and widespread distribution have been accompanied by an increase in the number of Salmonella infections reported to implicate contaminated food products. We developed a portable origami microfluidic device that
Yunxuan Chen, Yaxi Hu, Xiaonan Lu
semanticscholar +1 more source
การตรวจไวรัสมะเร็งเม็ดเลือดขาวในแมวที่รวดเร็วด้วยวิธี recombinase polymerase amplification (RPA)
2023โรคติดเชื้อไวรัสมะเร็งเม็ดเลือดขาวในแมว (Feline Leukemia Virus : FeLV) เป็นโรคติดเชื้อไวรัสในแมวที่สำคัญที่เป็นสาเหตุของโรคมะเร็งเม็ดเลือดขาว และ มะเร็งต่อมน้ำเหลือง การตรวจหา FeLV RNA และโปรไวรัส ด้วยวิธีทางอณูชีววิทยามีความสำคัญในการแยกระยะต่างๆของโรค ซึ่งวิธี Rapid immunochromatographic assay ที่นิยมใช้ตรวจแอนติเจนนั้นสามารถตรวจได้เพียงการติดเช ...
openaire +1 more source
International Journal of Infectious Diseases
Recombinase Polymerase Amplification (RPA) technology significantly advances the diagnostics of neglected tropical diseases (NTDs), providing rapid, isothermal, and minimally preparative testing ideally suited for under-resourced countries.
Luisa Hueso +7 more
semanticscholar +1 more source
Recombinase Polymerase Amplification (RPA) technology significantly advances the diagnostics of neglected tropical diseases (NTDs), providing rapid, isothermal, and minimally preparative testing ideally suited for under-resourced countries.
Luisa Hueso +7 more
semanticscholar +1 more source