Results 231 to 240 of about 131,465 (263)

Current status of recombinase polymerase amplification technologies for the detection of pathogenic microorganisms.

Diagnostic microbiology and infectious disease, 2023
Rapid detection of pathogenic microorganisms is key to the epidemiologic identification, prevention and control of disease in the field of public health.
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, X. Xia   +8 more
semanticscholar   +1 more source

Application of recombinant human pyruvate kinase in recombinase polymerase amplification

Journal of Bioscience and Bioengineering, 2023
Recombinase polymerase amplification (RPA) is an isothermal DNA amplification reaction at around 41°C using recombinase (Rec), single-stranded DNA-binding protein (SSB), strand-displacing DNA polymerase (Pol), and an ATP-regenerating enzyme. In this study, we attempted to use pyruvate kinase instead of creatine kinase (CK) that has been consistently ...
Kenji Kojima, Kenta Morimoto, Kevin Maafu Juma, Teisuke Takita, Kazuki Saito, Itaru Yanagihara, Shinsuke Fujiwara, Kiyoshi Yasukawa   +7 more
openaire   +2 more sources

Dual recombinase polymerase amplification system combined with lateral flow immunoassay for simultaneous detection of Staphylococcus aureus and Vibrio parahaemolyticus.

Journal of Pharmaceutical and Biomedical Analysis
Development of a highly sensitive visualization platform for multiplex genetic detection could significantly improve efficiency and reliability of on-site detection of foodborne pathogens. In this study, coupling recombinase polymerase amplification (RPA)
Yan Zhang, Xiaofeng Liu, Jia Luo, Hua Liu, You Li, Juan Liu, Lemei Zhu, Jinbin Wang, Haijuan Zeng   +8 more
semanticscholar   +1 more source

Recombinase polymerase amplification combined with Pyrococcus furiosus Argonaute for fast Salmonella spp. testing in food safety.

Journal of food microbiology
Foodborne illness caused by Salmonella spp. is one of the most prevalent public health problems globally, which have brought immeasurable economic burden and social impact to countries around the world.
Liyun Lin, Qiulan Luo, Liejun Li, Yuzhong Zheng, Huagui Wei, Jiayu Liao, Yaqun Liu, Mouquan Liu, Zhonghe Wang, Wanling Lin, Xianghui Zou, Hui Zhu, Min Lin   +12 more
semanticscholar   +1 more source

Multiplexed Recombinase Polymerase Amplification Assay To Detect Intestinal Protozoa

Analytical Chemistry, 2016
This work describes a proof-of-concept multiplex recombinase polymerase amplification (RPA) assay with lateral flow readout that is capable of simultaneously detecting and differentiating DNA from any of the diarrhea-causing protozoa Giardia, Cryptosporidium, and Entamoeba.
Zachary, Crannell, Alejandro, Castellanos-Gonzalez, Gayatri, Nair, Rojelio, Mejia, A Clinton, White, Rebecca, Richards-Kortum   +5 more
openaire   +2 more sources

Rapid authentication of characteristic milk powders by recombinase polymerase amplification assays.

Food Chemistry
The authentication of dairy species has great significance for food safety. This study focused on a more rapid method for identifying major dairy species, and specific recombinase polymerase amplification (RPA)-based assays for cattle, goat, sheep, camel
Cang Zhou, Libing Liu, Jia Chen, Qi Fu, Zhimin Chen, Jinfeng Wang, Xiaoxia Sun, Lianfeng Ai, Xiangdong Xu, Jianchang Wang   +9 more
semanticscholar   +1 more source

Sensitive and rapid detection of three foodborne pathogens in meat by recombinase polymerase amplification with lateral flow dipstick (RPA-LFD).

Journal of food microbiology
Foodborne illnesses, caused by harmful microorganisms in food, are a significant global health issue. Current methods for identifying these pathogens are both labor-intensive and time-consuming.
Wenxia Bai, Jie Chen, Dong Chen, Yike Zhu, Kairui Hu, Xiao Lin, Junlin Chen, Dafeng Song   +7 more
semanticscholar   +1 more source

Enhanced solid-phase recombinase polymerase amplification and electrochemical detection

Analytical and Bioanalytical Chemistry, 2017
Recombinase polymerase amplification (RPA) is an elegant method for the rapid, isothermal amplification of nucleic acids. Here, we elucidate the optimal surface chemistry for rapid and efficient solid-phase RPA, which was fine-tuned in order to obtain a maximum signal-to-noise ratio, defining the optimal DNA probe density, probe-to-lateral spacer ratio
Jonathan Sabaté, Del Río, Ivan Magriñà, Lobato, Olena, Mayboroda, Ioanis, Katakis, Ciara K, O'Sullivan   +4 more
openaire   +2 more sources

Optimization and Clinical Application Potential of Single Nucleotide Polymorphism Detection Method Based on CRISPR/Cas12a and Recombinase Polymerase Amplification.

Analytical Chemistry
Conventional methods for detecting single nucleotide polymorphisms (SNPs) in clinical practice often require substantial time, labor, and specialized equipment, limiting their widespread application.
Xingyue Wang, Ting Yang, Yunling Zhang, Z. Zeng, Qiang Wei, Pu Chen, Shuangshuang Yang, Yunfei Huang, Yongqi Zhang, Hongling Lu, Linhong Wu, Dijiao Tang, Ping Yang, Xuechun Wang, Qing Liu, Fan Li, Chao Ling, Shifeng Huang   +17 more
semanticscholar   +1 more source

Rapid, Sensitive, and Label-Free Detection of Long Noncoding RNAs in Breast Cancer Tissues by RecJf Exonuclease-Assisted Recombinase Polymerase Amplification.

Analytical Chemistry, 2023
An abnormal expression level of long noncoding RNAs (lncRNAs) is implicated in multiple cancers, and their sensitive and rapid measurement is pivotal for early cancer diagnosis and cancer treatment.
Su Jiang, Ting Liu, Qian Liu, Qian Zhang, Yunhong Han, Xiaorui Tian, Chun-Yang Zhang   +6 more
semanticscholar   +1 more source