Results 241 to 250 of about 131,465 (263)

Droplet digital recombinase polymerase amplification for multiplexed detection of human coronavirus.

Lab on a Chip, 2023
Since the outbreak of coronavirus 2019 (COVID-19), detection technologies have been attracting a great deal of attention in molecular diagnosis applications. In particular, the droplet digital PCR (ddPCR) has become a promising tool as it offers absolute
J. Choi, W. Seo, T. Kang, T. Kang, B. Chung   +4 more
semanticscholar   +1 more source

Integrating Recombinase Polymerase Amplification and Photosensitization Colorimetric Detection in One Tube for Fast Screening of C. sakazakii in Formula Milk Powder.

Analytical Chemistry
Cronobacter sakazakii (C. sakazakii) is a widely existing opportunistic pathogen and thus threatens people with low immunity, especially infants. To prevent the outbreak, a rapid and accurate on-site testing method is required.
Yanying Wang, Ting Zheng, Xianming Li, Peng Wu   +3 more
semanticscholar   +1 more source

FEN1-aided recombinase polymerase amplification (FARPA) for one-pot and multiplex detection of nucleic acids with an ultra-high specificity and sensitivity.

Biosensors & bioelectronics, 2023
Recombinase polymerase amplification (RPA) running at 37-42 °C is fast, efficient and less-implemented; however, the existing technologies of nucleic acid testing based on RPA have some limitations in specificity of single-base recognition and ...
Yinjiao Ma, Haiping Wu, Shan Chen, Chunmei Xie, Jingjing Hu, Xiemin Qi, Xueping Ma, Ya'nan Chu, Jingwen Shan, Yan Lu, L. Cui, Bingjie Zou, Guohua Zhou   +12 more
semanticscholar   +1 more source

Point-of-Care Testing for Norovirus Typing Using CRISPR/Cas12a Combined with Reverse Transcription Recombinase Polymerase Amplification

Bioconjugate chemistry, 2023
Noroviruses (NoVs) are one of the leading causes of acute gastroenteritis in humans. This study combined reverse transcription recombinase polymerase amplification (RT-RPA) with a clustered regularly interspaced short palindromic repeat/CRISPR-associated
Tanfen Fang, L. Zhang, Wei Ding, Yan Liu, P. Li, Wen Wang, Wenjing Xiang, Bo Wang, Wanping Sun   +8 more
semanticscholar   +1 more source

Development of a rapid, sensitive detection method for SARS‐CoV‐2 and influenza virus based on recombinase polymerase amplification combined with CRISPR‐Cas12a assay

Journal of Medical Virology, 2023
Respiratory tract infections are associated with the most common diseases transmitted among people and remain a huge threat to global public health. Rapid and sensitive diagnosis of causative agents is critical for timely treatment and disease control ...
Yuning Wang, Liqiang Wu, Xiaomei Yu, Gang Wang, Ting Pan, Zhao Huang, Ting-Ting Cui, Tianxun Huang, Zhen Huang, Libo Nie, Chungen Qian   +10 more
semanticscholar   +1 more source

Rapid Isothermal Detection of Pathogenic Clostridioides difficile Using Recombinase Polymerase Amplification.

Analytical Chemistry
Nosocomial-associated diarrhea due to Clostridioides difficile infection (CDI) is diagnosed after sample precultivation by the detection of the toxins in enzyme immunoassays or via toxin gene nucleic acid amplification.
Iris Bachmann, O. Behrmann, Marcel Klingenberg-Ernst, M. Rupnik, F. Hufert, G. Dame, M. Weidmann   +6 more
semanticscholar   +1 more source

Integrating recombinase polymerase amplification with CRISPR/Cas9-initiated nicking-rolling circle amplification in Staphylococcus aureus assay.

Chemical Communications
We integrate recombinase polymerase amplification (RPA) with CRISPR/Cas9-initiated nicking rolling circle amplification (CRISPR/Cas9-nRCA) for detecting Staphylococcus aureus.
Jianguo Xu, Tong Zhou, Danni Xue, Zhuqi Sui, Haidong Yang, Xinyue Yuan, Qi Wang   +6 more
semanticscholar   +1 more source

A specific and ultrasensitive Cas12a/crRNA assay with recombinase polymerase amplification and lateral flow biosensor technology for the rapid detection of Streptococcus pyogenes

Microbiology spectrum
The potential of CRISPR/Cas systems for nucleic acid detection in novel biosensing applications is remarkable. The current clinical diagnostic detection of Streptococcus pyogenes (S. pyogenes) is based on serological identification, culture, and PCR.
Yu-Hsuan Cheng, Jiawen Lyu, Jiang Han, Long Feng, Xiangmei Li, Pei Li, Shanfeng Zhang, Wenqiao Zang   +7 more
semanticscholar   +1 more source

Application of Recombinase Polymerase Amplification with CRISPR/Cas12a and Multienzyme lsothermal Rapid Amplification with lateral flow dipstick assay for Bactrocera correcta.

Pest Management Science
BACKGROUND Bactrocera correcta is a quarantine pest that negatively impacts the fruit and vegetable industry. Differentiating B. correcta from similar species, especially in non-adult stages, remains challenging. Rapid molecular identification techniques,
Weisong Li, Bo Cai, Ranran Chen, Jianchen Cui, Hui Wang, Zhihong Li   +5 more
semanticscholar   +1 more source

TE-RPA: One-tube telomerase extension recombinase polymerase amplification-based electrochemical biosensor for precise diagnosis of urothelial carcinoma.

Biosensors & bioelectronics
Telomerase demonstrates potential as a non-invasive urinary biomarker for urothelial carcinoma (UC); however, current detection methods are either labor-intensive or exhibit suboptimal performance.
Mengting Wang, Wenzhe Chen, Mingzhao Li, Fuyang Lin, J. Zhong, Wenrui Ouyang, Chao Cai, Guohua Zeng, Hongxing Liu   +8 more
semanticscholar   +1 more source