Results 131 to 140 of about 5,436 (163)
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Journal of Virological Methods, 2022
A rapid and simple real-time recombinase polymerase amplification (RPA) assay was developed to detect decapod iridescent virus 1 (DIV1). The assay was developed using optimized primers and probes designed from the conserved sequence of the DIV1 major capsid protein (MCP) gene.
Qian-Jun, Huang +9 more
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A rapid and simple real-time recombinase polymerase amplification (RPA) assay was developed to detect decapod iridescent virus 1 (DIV1). The assay was developed using optimized primers and probes designed from the conserved sequence of the DIV1 major capsid protein (MCP) gene.
Qian-Jun, Huang +9 more
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Lab on a Chip, 2010
For the first time we demonstrate a self-sufficient lab-on-a-foil system for the fully automated analysis of nucleic acids which is based on the recently available isothermal recombinase polymerase amplification (RPA). The system consists of a novel, foil-based centrifugal microfluidic cartridge including prestored liquid and dry reagents, and a ...
Lutz, Sascha +12 more
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For the first time we demonstrate a self-sufficient lab-on-a-foil system for the fully automated analysis of nucleic acids which is based on the recently available isothermal recombinase polymerase amplification (RPA). The system consists of a novel, foil-based centrifugal microfluidic cartridge including prestored liquid and dry reagents, and a ...
Lutz, Sascha +12 more
openaire +3 more sources
2015
Numerous molecular-based detection protocols include an amplification step of the targeted nucleic acids. This step is important to reach the expected sensitive detection of pathogens in diagnostic procedures. Amplifications of nucleic acid sequences are generally performed, in the presence of appropriate primers, using thermocyclers. However, the time
Glais, Laurent, Jacquot, Emmanuel
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Numerous molecular-based detection protocols include an amplification step of the targeted nucleic acids. This step is important to reach the expected sensitive detection of pathogens in diagnostic procedures. Amplifications of nucleic acid sequences are generally performed, in the presence of appropriate primers, using thermocyclers. However, the time
Glais, Laurent, Jacquot, Emmanuel
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Analytical Biochemistry, 2022
SENECAVIRUS A: (SVA), an emerging picornavirus, has been associated with vesicular disease and neonatal mortality in swine, posing a great threat to the global swine industry. Accurate diagnosis of SVA is crucial for the effective prevention and control disease. In the present study, a simple, rapid and accurate diagnostic assay was developed combining
Huibao, Wang +6 more
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SENECAVIRUS A: (SVA), an emerging picornavirus, has been associated with vesicular disease and neonatal mortality in swine, posing a great threat to the global swine industry. Accurate diagnosis of SVA is crucial for the effective prevention and control disease. In the present study, a simple, rapid and accurate diagnostic assay was developed combining
Huibao, Wang +6 more
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Lab on a Chip, 2021
A simple microfluidic-integrated lateral flow recombinase polymerase amplification (MI-IF-RPA) assay was developed for rapid COVID-19 detection.
Dan Liu +7 more
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A simple microfluidic-integrated lateral flow recombinase polymerase amplification (MI-IF-RPA) assay was developed for rapid COVID-19 detection.
Dan Liu +7 more
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Veterinary Parasitology, 2017
Toxoplasma gondii infects all warm-blooded vertebrates, resulting in a great threat to human health and significant economic loss to the livestock industry. Ingestion of infectious oocysts of T. gondii from the environment is the major source of transmission. Detection of T. gondii oocysts by existing methods is laborious, time-consuming and expensive.
Y D, Wu +6 more
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Toxoplasma gondii infects all warm-blooded vertebrates, resulting in a great threat to human health and significant economic loss to the livestock industry. Ingestion of infectious oocysts of T. gondii from the environment is the major source of transmission. Detection of T. gondii oocysts by existing methods is laborious, time-consuming and expensive.
Y D, Wu +6 more
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Journal of Microbiological Methods
A rapid real-time recombinase polymerase amplification (real-time RPA) assay was developed and validated for the detection of Staphylococcus aureus (S. aureus). The assay achieved target-specific amplification within 20 min at 39 °C and a 50% limit of detection (LOD₅₀) of 298 CFU/mL.
Qinglong, Wang +11 more
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A rapid real-time recombinase polymerase amplification (real-time RPA) assay was developed and validated for the detection of Staphylococcus aureus (S. aureus). The assay achieved target-specific amplification within 20 min at 39 °C and a 50% limit of detection (LOD₅₀) of 298 CFU/mL.
Qinglong, Wang +11 more
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Parasitology Research, 2018
Babesia gibsoni is a protozoan parasite responsible for the majority of reported cases of canine babesiosis in China. Currently, microscopic examination of the Giemsa-stained thin blood smears is the main diagnosis method in clinic. Here, we report the recombinase polymerase amplification-lateral flow (LF-RPA) dipstick detection method for targeting B.
Jie, Cui +8 more
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Babesia gibsoni is a protozoan parasite responsible for the majority of reported cases of canine babesiosis in China. Currently, microscopic examination of the Giemsa-stained thin blood smears is the main diagnosis method in clinic. Here, we report the recombinase polymerase amplification-lateral flow (LF-RPA) dipstick detection method for targeting B.
Jie, Cui +8 more
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The causative agent of "Surra", Trypanosoma brucei evansi (T. b. evansi), is thought to have originated from Trypanosoma brucei brucei (T. b. brucei) and primarily causes trypanosomosis in a wide range of wild and domesticated animals. The disease inflicts significant economic damage to farmers and the livestock industry. Additionally, T. b.
Li, Zeng +3 more
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Li, Zeng +3 more
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ปลาแซลมอนแอตแลนติก (Salmo salar) เป็นปลาที่นิยมนำมาบริโภคในรูปแบบเนื้อปลาดิบ เนื่องจากมีรสชาติดีและคุณค่าทางโภชนาการสูง อย่างไรก็ดี เป็นที่ทราบกันดีว่าปลาแซลมอนแอตแลนติกในปัจจุบันมีอัตราการผลิตลดต่ำลง ส่งผลให้มีราคาสูงขึ้น เป็นเหตุให้มีการนำปลาที่มีลักษณะเนื้อคล้ายคลึงกัน อย่างเช่น ปลาเรนโบว์เทราต์ (Oncorhynchus mykiss) มาปลอมปน ซึ่งหากปรากฏลักษณะทางส ...
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