Results 81 to 90 of about 2,445,999 (304)

Regulation of the lncRNA NEAT1 by p53‐ΔNp63 crosstalk modulates the DNA damage response and therapeutic efficacy in HNSCC

Molecular Oncology, EarlyView.
In head and neck squamous cell carcinoma (HNSCC) p53 and p63 exert opposite roles on the transcription regulation of the lncRNA NEAT1. Under basal conditions, p53 levels are low and p63 represses NEAT1 expression. Upon genotoxic stress, p53 is rapidly induced, displacing p63 from the NEAT1 promoter leading to NEAT1 transcriptional activation and ...
Sara De Domenico, Veronica La Banca, Silvia D'Amico, Stefano Scalera, Sara Nicolai, Angelo Peschiaroli   +5 more
wiley   +1 more source

Clonality despite sex: the evolution of host-associated sexual neighborhoods in the pathogenic fungus Penicillium marneffei.

PLoS Pathogens, 2012
Molecular genetic approaches typically detect recombination in microbes regardless of assumed asexuality. However, genetic data have shown the AIDS-associated pathogen Penicillium marneffei to have extensive spatial genetic structure at local and ...
Daniel A Henk, Revital Shahar-Golan, Khuraijam Ranjana Devi, Kylie J Boyce, Nengyong Zhan, Natalie D Fedorova, William C Nierman, Po-Ren Hsueh, Kwok-Yung Yuen, Tran P M Sieu, Nguyen Van Kinh, Heiman Wertheim, Stephen G Baker, Jeremy N Day, Nongnuch Vanittanakom, Elaine M Bignell, Alex Andrianopoulos, Matthew C Fisher   +17 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Function of bacteriophage orf recombinasesin genetic exchange [PDF]

, 2006
Recombination events in bacteriophages frequently occur by illegitimate exchange at short tracts of sequence homology, enabling these viruses to acquire novel genes and serve as vehicles for horizontal gene transfer.
Reed, Patricia
core  

Clonal diversity of the glutamate dehydrogenase gene in Giardia duodenalis from Thai Isolates: evidence of genetic exchange or Mixed Infections? [PDF]

, 2011
Background: The glutamate dehydrogenase gene (gdh) is one of the most popular and useful genetic markers for the genotypic analysis of Giardia duodenalis (syn. G. lamblia, G. intestinalis), the protozoan that widely causes enteric disease in humans.
Boontanom, P., Mungthin, M., Thompson, R.C.A., Tan-ariya, P., Siripattanapipong, S., Saksirisampant, W., Mathirut Mungthin, Peerapan Tan-ariya, RC Andrew Thompson, Leelayoova, S., Suradej Siripattanapipong, Wilai Saksirisampant, Parima Boontanom, Saovanee Leelayoova   +13 more
core   +1 more source

Fungal clones win the battle, but recombination wins the war

IMA Fungus, 2019
Clonal reproduction is common in fungi and fungal-like organisms during epidemics and invasion events. The success of clonal fungi shaped systems for their classification and some pathogens are tacitly treated as asexual.
André Drenth, Alistair R. McTaggart, Brenda D. Wingfield   +2 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Genetic Engineering by DNA Recombineering

Current Protocols in Chemical Biology, 2019
AbstractRecombineering inserts PCR products into DNA using homologous recombination. A pair of short homology arms (50 base pairs) on the ends of a PCR cassette target the cassette to its intended location. These homology arms can be easily introduced as 5′ primer overhangs during the PCR reaction.
Papa, Louis John, Shoulders, Matthew D.
openaire   +5 more sources

Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region

FEBS Open Bio, EarlyView.
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson, Anja H. Schiemann, Evelyn Sattlegger   +2 more
wiley   +1 more source

Diversity and recombination in Wolbachia and Cardinium from Bryobia spider mites [PDF]

, 2012
Background Wolbachia and Cardinium are endosymbiotic bacteria infecting many arthropods and manipulating host reproduction. Although these bacteria are maternally transmitted, incongruencies between phylogenies of host and parasite suggest an additional ...
Vera I D Ros, Edward J Feil, Johannes A J Breeuwer, Breeuwer, J.A.J., Fleming, V.M., Feil, E.J., Ros, V.I.D., Vicki M Fleming   +7 more
core   +2 more sources