Results 121 to 130 of about 6,124,322 (291)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Data-driven reduced order model and simplicial homology global optimization for reliability analysis and application. [PDF]

Heliyon, 2022
Zhao H, Wang M, Chang X.
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Development of Reduced Order Model for Steady State Flow Field in Two-dimensional Lower Plenum

Yuanzineng kexue jishu
In the field of reactor thermal-hydraulics, system thermal-hydraulic codes have been widely employed to quickly determine the global response characteristics of the primary system.
SHANG He1, PENG Minjun1, 2, XIA Genglei1, 2, , ZHANG Shiqi1, ZHU Shijie1
doaj   +1 more source

Semi-mechanistic reduced order model of pharmaceutical tablet dissolution for enabling Industry 4.0 manufacturing systems. [PDF]

Int J Pharm, 2023
Ferdoush S, Gonzalez M.
europepmc   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Development of a force identification method using system-level measurements with component-level reduced-order modeling

Nuclear Engineering and Technology
The accurate estimation of excitation forces is essential for maintaining the structural integrity of dynamic systems. However, direct measurement of excitation forces is often infeasible in practical scenarios.
Hyeongyeong Jin, Seunghun Baek
doaj   +1 more source

A multiparametric advection-diffusion reduced-order model for molecular transport in scaffolds for osteoinduction. [PDF]

Biomech Model Mechanobiol, 2022
Muixí A, Zlotnik S, Calvet P, Espanol M, Lodoso-Torrecilla I, Ginebra MP, Díez P, García-González A.   +7 more
europepmc   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Reduced-Order Model of a Time-Trial Cyclist Helmet for Aerodynamic Optimization Through Mesh Morphing and Enhanced with Real-Time Interactive Visualization

Fluids
Aerodynamics is a key factor in time-trial cycling. Over the years, various aspects have been investigated, including positioning, clothing, bicycle design, and helmet shape.
E. Di Meo, A. Lopez, C. Groth, M. E. Biancolini, P. P. Valentini   +4 more
doaj   +1 more source