Results 101 to 110 of about 3,004 (180)

Characterization of the Corrinoid Iron-Sulfur ProteinTetrachloroethene Reductive Dehalogenase of Dehalobacterrestrictus [PDF]

open access: yesApplied and Environmental Microbiology, 2003
ABSTRACT The membrane-bound tetrachloroethene reductive dehalogenase (PCE-RDase) (PceA; EC 1.97.1.8), the terminal component of the respiratory chain of Dehalobacter restrictus , was purified 25-fold to apparent electrophoretic homogeneity. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed
Maillard Julien   +5 more
openaire   +3 more sources

Identification of stable endogenous reference genes for real-time PCR in the human fetal gonad using an external standard technique [PDF]

open access: yes, 2011
Peer ...
Ana Monteiro   +13 more
core   +1 more source

Development of a Continuous, Uncoupled Fluorescence‐based Assay for Monitoring Methyltransferase Activity: Methylation Going Sour

open access: yesChemCatChem, Volume 18, Issue 3, 12 February 2026.
Methylation going sour: The first uncoupled fluorescence‐based assay was established to measure methyltransferase activity in a continuous fashion. Different use cases are presented, including the assay's compatibility with catalyst formulations other than purified enzymes, the determination of kinetic parameters, as well as the screening of a ...
Benjamin Panagiotis Chapple   +2 more
wiley   +1 more source

19F NMR as a Molecular Reporter: Labelling Strategies and Methods for Probing Biomolecular Motion and Exchange

open access: yesChemistry–Methods, Volume 6, Issue 2, February 2026.
This review summarizes the unique benefits 19F NMR spectroscopy has to offer for the study of biological systems. Particular focus is laid on the available strategies for incorporation of fluorinated probes into proteins and the latest applications of the ever‐expanding toolbox of fluorine NMR methodology to study structure and dynamics.
Charles Burridge, Björn M. Burmann
wiley   +1 more source

Desulfitobacterium elongatum sp. nov. NIT-TF6 Isolated from Trichloroethene-Dechlorinating Culture with Formate

open access: yesMicroorganisms
A strictly anaerobic bacterium denoted as strain NIT-TF6 of the genus Desulfitobacterium was isolated from a trichloroethene-dechlorinating culture with formate.
Udaratta Bhattacharjee   +3 more
doaj   +1 more source

Frequent concomitant presence of Desulfitobacterium spp. and " Dehalococcoides ” spp. in chloroethene-dechlorinating microbial communities [PDF]

open access: yes, 2018
The presence of chloroethene dechlorination activity as well as several bacterial genera containing mainly organohalide-respiring members was investigated in 34 environmental samples from 18 different sites.
Holliger, Christof   +2 more
core  

Exploring the Potential and Evaluating Hydrocarbon Degradation by Novel Antarctic Dietzia and Pusillimonas Isolates From a Pristine Environment

open access: yesEnvironmental Microbiology Reports, Volume 18, Issue 1, February 2026.
The Antarctic Dietzia and Pusillimonas isolates were tested for bioremediation applications. Genomic analyses revealed the potential to degrade complex organic compounds, potential virulence factors and resistance genes. Physiological assays showed a wide range of adaptation to abiotic factors and sensitivity to different groups of antibiotics ...
Tomasz Krucoń   +4 more
wiley   +1 more source

Experimental determination of isotope enrichment factors – bias from mass removal by repetitive sampling [PDF]

open access: yes, 2016
Application of compound-specific stable isotope approaches often involves comparisons of isotope enrichment factors (ε). Experimental determination of ε-values is based on the Rayleigh equation, which relates the change in measured isotope ratios to the ...
Buchner, Daniel   +5 more
core   +2 more sources

Microbiome dynamics of a polychlorobiphenyl (PCB) historically contaminated marine sediment under conditions promoting reductive dechlorination

open access: yesFrontiers in Microbiology, 2016
The toxicity of polychlorinated biphenyls (PCB) can be efficiently reduced in contaminated marine sediments through the reductive dechlorination process lead by anaerobic organohalide bacteria.
Bruna Matturro   +2 more
doaj   +1 more source

Molecular basis of cobalamin-dependent RNA modification [PDF]

open access: yes, 2016
Queuosine (Q) was discovered in the wobble position of a transfer RNA (tRNA) 47 years ago, yet the final biosynthetic enzyme responsible for Q-maturation, epoxyqueuosine (oQ) reductase (QueG), was only recently identified.
Bandarian, Vahe   +6 more
core   +1 more source

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