Results 91 to 100 of about 3,004 (180)

StabLyzeGraph: High‐throughput screening of combinatorial mutations using graph neural networks

Protein Science, Volume 35, Issue 4, April 2026.
Abstract Engineering protein stability is a powerful strategy across biotechnology and medicine, supporting a broad range of applications such as atomic structure determination, discovery of therapeutic molecules, biomanufacturing, diagnostic reagents, industrial biocatalysis, etc.
Muhammad Waqas, Benito Natale, Michele Roggia, Prasenjit Prasad Saha, Mauro Mileni, Sandro Cosconati   +5 more
wiley   +1 more source

Detection of Organohalide-Respiring Enzyme Biomarkers at a Bioaugmented TCE-Contaminated Field Site

Frontiers in Microbiology, 2019
RNA-based biomarkers have been successfully detected at field sites undergoing in situ bioremediation, but the detection of expressed enzymes is a more direct way to prove activity for a particular biocatalytic process of interest since they provide ...
Gretchen L. W. Heavner, Cresten B. Mansfeldt, Michael J. Wilkins, Carrie D. Nicora, Garrett E. Debs, Elizabeth A. Edwards, Ruth E. Richardson   +6 more
doaj   +1 more source

Uneven distribution of cobamide biosynthesis and dependence in bacteria predicted by comparative genomics. [PDF]

, 2019
The vitamin B12 family of cofactors known as cobamides are essential for a variety of microbial metabolisms. We used comparative genomics of 11,000 bacterial species to analyze the extent and distribution of cobamide production and use across bacteria ...
Haft, David R, Han, Andrew W, Jackson, Samantha N, Mok, Kenny C, Seth, Erica C, Shelton, Amanda N, Taga, Michiko E   +6 more
core   +1 more source

SegDesign: A modular framework for controllable protein segment engineering

Protein Science, Volume 35, Issue 4, April 2026.
Abstract Local protein structure engineering, such as targeted remodeling of loops or flexible regions, is critical for mechanistic studies and protein optimization but remains challenging to perform in a controllable and reproducible manner. Despite advances in protein foundation models and generative structure design, most existing methods emphasize ...
Chenjie Feng, Junbo Yin, Chao Zha, Mohammed Saif, Xiaopeng Xu, Xin Gao, Wenjia He   +6 more
wiley   +1 more source

Investigations on a chloroform reductive dehalogenase

, 2017
Halogenated organic compounds (organohalides) are globally prevalent, recalcitrant toxic and carcinogenic environmental pollutants contaminating soil and groundwater. Organohalide respiring bacteria provide a potential solution to remediate contaminated sites, as they are capable of utilising organohalides as electron acceptors for the generation of ...
openaire   +2 more sources

Metagenomes from Coastal Marine Sediments Give Insights into the Ecological Role and Cellular Features of Loki- and Thorarchaeota

mBio, 2019
The genomes of Asgard Archaea, a novel archaeal proposed superphylum, share an enriched repertoire of eukaryotic signature genes and thus promise to provide insights into early eukaryote evolution.
Lokeshwaran Manoharan, Jessica A. Kozlowski, Robert W. Murdoch, Frank E. Löffler, Filipa L. Sousa, Christa Schleper   +5 more
doaj   +1 more source

From Energy Deprivation Stress to Catalytic Adaptation of Reductive Dehalogenase TmrA for 1,1‐DCA Dechlorination: An Experimental and in Silico Study

Microbial Biotechnology, Volume 19, Issue 4, April 2026.
Adaptive evolution of Dehalobacter restrictus strain UNSWDHB under a ratio of 1:9 chloroform:1,1‐DCA regime increased 1,1‐DCA dechlorination. Sequencing and molecular dynamics simulation reveal high‐entropy tmrA mutations that improved 1,1‐DCA binding energy and stabilize the catalytic pocket, outlining a route to tune reductive dehalogenases for ...
Yi Ren, Evan Landers, Matthew Lee, Mike Manefield   +3 more
wiley   +1 more source

An α/β‐Hydrolase GmABHD6 Controls Seed Oil Content and Yield in Soybean

Plant Biotechnology Journal, Volume 24, Issue 4, Page 2040-2055, April 2026.
ABSTRACT Seed oil content is a key trait in soybean, yet its genetic basis remains largely unresolved. Here, we identify GmABHD6, an α/β‐hydrolase domain‐containing gene, as the causal gene underlying a major quantitative trait locus (QTL) for seed oil content on chromosome 16.
Kaixin Yu, Limin Hu, Bo Sun, Zehao Chen, Xu Li, Shiyu Zhang, Huilin Tian, Wenjing Zhang, Shimin Ren, Xue Han, Chang Xu, Siming Wei, Mingliang Yang, Fanjiang Kong, Qingshan Chen, Zhaoming Qi   +15 more
wiley   +1 more source

Insights Into the Biodegradation of Lindane (γ-Hexachlorocyclohexane) Using a Microbial System

Frontiers in Microbiology, 2020
Lindane (γ-hexachlorocyclohexane) is an organochlorine pesticide that has been widely used in agriculture over the last seven decades. The increasing residues of lindane in soil and water environments are toxic to humans and other organisms.
Wenping Zhang, Wenping Zhang, Ziqiu Lin, Ziqiu Lin, Shimei Pang, Shimei Pang, Pankaj Bhatt, Pankaj Bhatt, Shaohua Chen, Shaohua Chen   +9 more
doaj   +1 more source

Geochemical and microbial community determinants of reductive dechlorination at a site biostimulated with glycerol [PDF]

, 2016
Biostimulation is widely used to enhance reductive dechlorination of chlorinated ethenes in contaminated aquifers. However, the knowledge on corresponding biogeochemical responses is limited. In this study glycerol was injected in an aquifer contaminated
Abelson, Aeppli, Anderson, Atashgahi, Atashgahi, Bernardet, Bernardet, Biebl, Bowman, Briée, Brown, Campbell, Canfield, Caporaso, Clarke, Damgaard, Daprato, Dennis, Dojka, Dugat-Bony, Duhamel, Duhamel, Eisenmann, Ellis, Elshahed, Freeborn, Gelder, Gihring, Gonzáles, Goris, Goris, Gu, Hamonts, Handley, Handley, He, He, Hendrickson, Hoelen, Hohnstock-Ashe, Hug, Imfeld, Imfeld, Kao, Kielhorn, Kocur, Kodama, Kodama, Lee, Lee, Lendvay, Leys, Lowe, Luijten, Luo, Lê Cao, Löffler, Löffler, Macbeth, Maillard, Major, Men, Men, Men, Merlino, Moe, Peura, Puigserver, Pérez-De-Mora, Qatibi, Qatibi, Quast, Rabus, Rahm, Ramiro-Garcia, Richardson, Ritalahti, Rossi, Rowe, Révész, Scheutz, Scheutz, Schipp, Schneidewind, Shade, Smatlak, Stroo, Sutton, Sutton, Tsai, Wilkens, Wrighton, Wrighton, Yan, Yan, Yang, Zhou, Zhuang   +97 more
core   +2 more sources