Selective enrichment yields robust ethene-producing dechlorinating cultures from microcosms stalled at cis-dichloroethene. [PDF]
Dehalococcoides mccartyi strains are of particular importance for bioremediation due to their unique capability of transforming perchloroethene (PCE) and trichloroethene (TCE) to non-toxic ethene, through the intermediates cis-dichloroethene (cis-DCE ...
Anca G Delgado +7 more
doaj +1 more source
Microbial Community Dynamics in Marine Water: Influence of Hydrocarbon Type and Exposure Time
ABSTRACT Marine environments are frequently impacted by petroleum‐derived hydrocarbons, which pose ecological risks because of their toxic, mutagenic, and carcinogenic potential. Microorganisms play a crucial role in hydrocarbon degradation, and understanding the influence of time and hydrocarbon type on microbial dynamics helps improve bioremediation ...
Karen C. F. Santaren +4 more
wiley +1 more source
Overview of organohalide-respiring bacteria and a proposal for a classification system for reductive dehalogenases [PDF]
Organohalide respiration is an anaerobic bacterial respiratory process that uses halogenated hydrocarbons as terminal electron acceptors during electron transport-based energy conservation. This dechlorination process has triggered considerable interest for detoxification of anthropogenic groundwater contaminants.
Hug, L.A. +6 more
openaire +2 more sources
Background 1,2-dichloroethane (1,2-DCA) biodegradation can occur through aerobic or anaerobic pathways that can be exploited in bioremediation strategies.
Ilenia Cruciata +7 more
doaj +1 more source
Bioorthogonal Chemistry in Neurobiology: From Imaging to Proteomics
This review highlights emerging strategies for labeling neuronal proteins, enabling visualization and quantitative analysis of their expression, localization, and dynamics. By combining genetic code expansion (GCE), metabolic labeling, and click chemistry‐based labeling, these approaches provide powerful routes to study proteins in specific cells and ...
Neil Taylor +2 more
wiley +1 more source
Multiple Nonidentical Reductive-Dehalogenase-Homologous Genes Are Common in Dehalococcoides [PDF]
ABSTRACT Degenerate primers were used to amplify large fragments of reductive-dehalogenase-homologous (RDH) genes from genomic DNA of two Dehalococcoides populations, the chlorobenzene- and dioxin-dechlorinating strain CBDB1 and the trichloroethene-dechlorinating strain FL2.
Tina, Hölscher +6 more
openaire +2 more sources
Detection of Organohalide-Respiring Enzyme Biomarkers at a Bioaugmented TCE-Contaminated Field Site
RNA-based biomarkers have been successfully detected at field sites undergoing in situ bioremediation, but the detection of expressed enzymes is a more direct way to prove activity for a particular biocatalytic process of interest since they provide ...
Gretchen L. W. Heavner +6 more
doaj +1 more source
Predicting Functional Diversity Within Enzyme Families: A Practical Perspective
Integrating machine learning into enzyme discovery requires navigating the trade‐offs between data quantity and quality. This article provides a best‐practice framework for developing robust predictors, focusing on mitigating bias in biochemical datasets, implementing systematic “cold‐drug” validation strategies, and utilizing explainable artificial ...
Nika Sokolova, Kristina Haslinger
wiley +1 more source
Investigations on a chloroform reductive dehalogenase
Halogenated organic compounds (organohalides) are globally prevalent, recalcitrant toxic and carcinogenic environmental pollutants contaminating soil and groundwater. Organohalide respiring bacteria provide a potential solution to remediate contaminated sites, as they are capable of utilising organohalides as electron acceptors for the generation of ...
openaire +2 more sources
Background Industrial waste, agricultural runoff and untreated sewage contaminate the Nile, leaving a toxic legacy in its sediments. Organohalides-polluted sediment in particular poses serious public health risks and detrimental effects on aquatic life ...
Hwayda Soliman +3 more
doaj +1 more source

