Application of nitroarene dioxygenases in the design of novel strains that degrade chloronitrobenzenes. [PDF]
Widespread application of chloronitrobenzenes as feedstocks for the production of industrial chemicals and pharmaceuticals has resulted in extensive environmental contamination with these toxic compounds, where they pose significant risks to the health ...
Ju, Kou-San, Parales, Rebecca E
core +1 more source
Multiple Nonidentical Reductive-Dehalogenase-Homologous Genes Are Common in Dehalococcoides [PDF]
ABSTRACT Degenerate primers were used to amplify large fragments of reductive-dehalogenase-homologous (RDH) genes from genomic DNA of two Dehalococcoides populations, the chlorobenzene- and dioxin-dechlorinating strain CBDB1 and the trichloroethene-dechlorinating strain FL2.
Tina, Hölscher +6 more
openaire +2 more sources
Pentachlorophenol and spent engine oil degradation by Mucor ramosissimus [PDF]
Pentachlorophenol (PCP) has been widely used for many years and belongs to the most toxic pollutants. Spent engine oils enter environment every day in many ways. Both of them cause great environmental concern.
Długoński, Jerzy, Szewczyk, Rafał
core +1 more source
PFAS properties and destruction methods: A focus on enzymatic degradation
Abstract Per‐ and polyfluoroalkyl substances (PFAS) are known as ‘forever’ chemicals and have lasting detrimental impact on the environment and living organisms. To understand PFAS molecules better, this review begins with an overview of PFAS definition, classifications and applications, and then provides a comprehensive summary and critical analysis ...
Guobin Liang, Hua Zhao
wiley +1 more source
Contrasting Kinetics of Highly Similar Chloroalkane Reductive Dehalogenases
ABSTRACTChloroform and trichloroethanes are pervasive groundwater contaminants for which bioremediation has been an effective treatment strategy. Reductive dehalogenase (RDase) enzymes from organohalide-respiring bacteria are essential for their remediation under anaerobic conditions. RDases are responsible for dehalogenating these chlorinated solvents,
Katherine J. Picott +1 more
openaire +2 more sources
Identification of a Chlorobenzene Reductive Dehalogenase in Dehalococcoides sp. Strain CBDB1 [PDF]
ABSTRACT A chlorobenzene reductive dehalogenase of the anaerobic dehalorespiring bacterium Dehalococcoides sp. strain CBDB1 was identified. Due to poor biomass yields, standard protein isolation procedures were not applicable. Therefore, cell extracts from cultures grown on trichlorobenzenes were separated
Adrian, L. +3 more
openaire +3 more sources
Quantification of genes and gene transcripts for microbial perchlorate reduction in fixed‐bed bioreactors [PDF]
Aims: Optimization of full‐scale, biological perchlorate treatment processes for drinking water would benefit from knowledge of the location and quantity of perchlorate‐reducing bacteria (PRB) and expression of perchlorate‐related genes in bioreactors.
Bender +33 more
core +1 more source
Microbial Community Dynamics in Marine Water: Influence of Hydrocarbon Type and Exposure Time
ABSTRACT Marine environments are frequently impacted by petroleum‐derived hydrocarbons, which pose ecological risks because of their toxic, mutagenic, and carcinogenic potential. Microorganisms play a crucial role in hydrocarbon degradation, and understanding the influence of time and hydrocarbon type on microbial dynamics helps improve bioremediation ...
Karen C. F. Santaren +4 more
wiley +1 more source
Functional Characterization of Reductive Dehalogenases by Using Blue Native Polyacrylamide Gel Electrophoresis [PDF]
ABSTRACT Dehalococcoides mccartyi strains are obligate organohalide-respiring bacteria harboring multiple distinct reductive dehalogenase (RDase) genes within their genomes. A major challenge is to identify substrates for the enzymes encoded by these RDase genes.
Tang +7 more
openaire +2 more sources
Engineering Fluoroacetate Dehalogenase by Growth‐Based Selections on Non‐Natural Organofluorides
We present a high‐throughput selection system to engineer fluoroacetate dehalogenases (FAcDs). By challenging E. coli populations that produce diverse FAcD libraries to grow on non‐natural organofluorides as their sole carbon source, we isolated a panel of FAcD variants with improved activity and altered substrate specificity.
Suzanne C. Jansen +2 more
wiley +2 more sources

