Results 41 to 50 of about 83,987 (180)

Sister Dehalobacter Genomes Reveal Specialization in Organohalide Respiration and Recent Strain Differentiation Likely Driven by Chlorinated Substrates

Frontiers in Microbiology, 2016
The genomes of two closely related Dehalobacter strains (strain CF and strain DCA) were assembled from the metagenome of an anaerobic enrichment culture that reductively dechlorinates chloroform (CF), 1,1,1-trichloroethane (1,1,1-TCA) and 1,1 ...
Shuiquan eTang, Po Hsiang eWang, Steven eHiggins, Steven eHiggins, Frank eLoeffler, Frank eLoeffler, Elizabeth Anne Edwards   +6 more
doaj   +1 more source

A common mechanism for coenzyme cobalamin-dependent reductive dehalogenases

Phys. Chem. Chem. Phys., 2017
Distinct mechanisms have been proposed for the biological dehalogenation catalyzed by cobalamin-dependent enzymes, with two recent crystallographic studies suggesting different mechanisms based on the observed interaction between the organohalide substrate and cobalamin.
Linus O. Johannissen, David Leys, Sam Hay   +2 more
openaire   +3 more sources

Isolation and genomic characterization of a Dehalococcoides strain suggests genomic rearrangement during culture

Scientific Reports, 2017
We have developed and characterized a bacterial consortium that reductively dechlorinates trichloroethene to ethene. Quantitative PCR analysis for the 16S rRNA and reductive dehalogenase genes showed that the consortium is highly enriched with ...
Masafumi Yohda, Kentaro Ikegami, Yuto Aita, Mizuki Kitajima, Ayane Takechi, Megumi Iwamoto, Tomomi Fukuda, Noriyoshi Tamura, Junji Shibasaki, Seiji Koike, Daisuke Komatsu, Sakari Miyagi, Minoru Nishimura, Yoshihito Uchino, Akino Shiroma, Makiko Shimoji, Hinako Tamotsu, Noriko Ashimine, Misuzu Shinzato, Shun Ohki, Kazuma Nakano, Kuniko Teruya, Kazuhito Satou, Takashi Hirano, Osami Yagi   +24 more
doaj   +1 more source

Characterization of a new G-type halohydrin dehalogenase with enhanced catalytic activity [PDF]

, 2022
Biochemical characterization of HheG-682, a new homolog of halohydrin dehalogenase HheG sharing 46% sequence identity, is ...
Sophie, Günther, Anett, Schallmey
core   +1 more source

Unveiling the Complete Biosynthetic Pathway of Azacitidine: A Rare 1,3,5‐Triazine Nucleoside Derived From GTP

Angewandte Chemie, EarlyView.
The azacitidine biosynthetic gene cluster (aza BGC) was identified by the resistance‐gene‐guided comparative genomics method in Streptomyces mobaraensis IPIO2. The complete biosynthetic pathway of azacitidine, which follows a “base‐first‐then‐glycosylation” logic, was elucidated through in vivo and in vitro experiments.
Dan Wang, Qingxu Meng, Lingqi Hua, Mengjun Sun, Qianjin Kang, Min‐Juan Xu, Linquan Bai   +6 more
wiley   +2 more sources

Proteomic data set of the organohalide-respiring Epsilonproteobacterium Sulfurospirillum multivorans adapted to tetrachloroethene and other energy substrates

Data in Brief, 2016
Sulfurospirillum multivorans is a free-living, physiologically versatile Epsilonproteobacterium able to couple the reductive dehalogenation of chlorinated and brominated ethenes to growth (organohalide respiration).
Tobias Goris, Christian L. Schiffmann, Jennifer Gadkari, Lorenz Adrian, Martin von Bergen, Gabriele Diekert, Nico Jehmlich   +6 more
doaj   +1 more source

Functional Expression and Characterization of Tetrachloroethene Dehalogenase From Geobacter sp.

Frontiers in Microbiology, 2018
Reductive dehalogenase (RDase) consists of two parts, RdhA and RdhB. RdhA is the catalytic subunit, harboring a cobalamin cofactor and two Fe–S clusters. RdhA is anchored to the cytoplasmic membrane via the membrane anchoring subunit, RdhB.
Ryuki Nakamura, Tomohiro Obata, Ryota Nojima, Yohey Hashimoto, Keiichi Noguchi, Takahiro Ogawa, Masafumi Yohda, Masafumi Yohda   +7 more
doaj   +1 more source

Initial Characterization of a Reductive Dehalogenase from Desulfitobacterium chlororespirans Co23 [PDF]

Applied and Environmental Microbiology, 1996
Desulfitobacterium chlororespirans Co23 is capable of using 3-chloro-4-hydroxybenzoate as terminal electron acceptor for growth. Membrane preparations from cells grown fermentatively on pyruvate in the presence of 3-chloro-4-hydroxybenzoate dechlorinated this compound at a rate of 3.9 nmol min(sup-1) mg of protein(sup-1).
F E, Loffler, R A, Sanford, J M, Tiedje
openaire   +2 more sources

The Membrane-Bound C Subunit of Reductive Dehalogenases: Topology Analysis and Reconstitution of the FMN-Binding Domain of PceC

Frontiers in Microbiology, 2018
Organohalide respiration (OHR) is the energy metabolism of anaerobic bacteria able to use halogenated organic compounds as terminal electron acceptors.
Géraldine F. Buttet, Mathilde S. Willemin, Romain Hamelin, Aamani Rupakula, Julien Maillard   +4 more
doaj   +1 more source

Genomic Characteristics Distinguish Geographically Distributed Dehalococcoidia

Frontiers in Microbiology, 2020
Dehalococcoidia (Dia) class microorganisms are frequently found in various pristine and contaminated environments. Metagenome-assembled genomes (MAGs) and single-cell amplified genomes (SAGs) studies have substantially improved the understanding of Dia ...
Yi Yang, Yaozhi Zhang, Yaozhi Zhang, Natalie L. Cápiro, Jun Yan   +4 more
doaj   +1 more source