Results 31 to 40 of about 1,116 (113)

Proteomic data set of the organohalide-respiring Epsilonproteobacterium Sulfurospirillum multivorans adapted to tetrachloroethene and other energy substrates

Data in Brief, 2016
Sulfurospirillum multivorans is a free-living, physiologically versatile Epsilonproteobacterium able to couple the reductive dehalogenation of chlorinated and brominated ethenes to growth (organohalide respiration).
Tobias Goris, Christian L. Schiffmann, Jennifer Gadkari, Lorenz Adrian, Martin von Bergen, Gabriele Diekert, Nico Jehmlich   +6 more
doaj   +1 more source

Functional Expression and Characterization of Tetrachloroethene Dehalogenase From Geobacter sp.

Frontiers in Microbiology, 2018
Reductive dehalogenase (RDase) consists of two parts, RdhA and RdhB. RdhA is the catalytic subunit, harboring a cobalamin cofactor and two Fe–S clusters. RdhA is anchored to the cytoplasmic membrane via the membrane anchoring subunit, RdhB.
Ryuki Nakamura, Tomohiro Obata, Ryota Nojima, Yohey Hashimoto, Keiichi Noguchi, Takahiro Ogawa, Masafumi Yohda, Masafumi Yohda   +7 more
doaj   +1 more source

The Membrane-Bound C Subunit of Reductive Dehalogenases: Topology Analysis and Reconstitution of the FMN-Binding Domain of PceC

Frontiers in Microbiology, 2018
Organohalide respiration (OHR) is the energy metabolism of anaerobic bacteria able to use halogenated organic compounds as terminal electron acceptors.
Géraldine F. Buttet, Mathilde S. Willemin, Romain Hamelin, Aamani Rupakula, Julien Maillard   +4 more
doaj   +1 more source

Genomic Characteristics Distinguish Geographically Distributed Dehalococcoidia

Frontiers in Microbiology, 2020
Dehalococcoidia (Dia) class microorganisms are frequently found in various pristine and contaminated environments. Metagenome-assembled genomes (MAGs) and single-cell amplified genomes (SAGs) studies have substantially improved the understanding of Dia ...
Yi Yang, Yaozhi Zhang, Yaozhi Zhang, Natalie L. Cápiro, Jun Yan   +4 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Organohalide Respiring Bacteria and Reductive Dehalogenases: Key Tools in Organohalide Bioremediation

Frontiers in Microbiology, 2016
Organohalides are recalcitrant pollutants that have been responsible for substantial contamination of soils and groundwater. Organohalide-respiring bacteria (ORB) provide a potential solution to remediate contaminated sites, through their ability to use ...
Bat-Erdene eJugder, Haluk eErtan, Haluk eErtan, Susanne eBohl, Matthew eLee, Susanne eBohl, Christopher Peter Marquis, Michael eManefield   +7 more
doaj   +1 more source

Microbial Community Dynamics in Marine Water: Influence of Hydrocarbon Type and Exposure Time

Environmental Toxicology, EarlyView.
ABSTRACT Marine environments are frequently impacted by petroleum‐derived hydrocarbons, which pose ecological risks because of their toxic, mutagenic, and carcinogenic potential. Microorganisms play a crucial role in hydrocarbon degradation, and understanding the influence of time and hydrocarbon type on microbial dynamics helps improve bioremediation ...
Karen C. F. Santaren, Douglas A. Monteiro, Eliene S. Lopes, Diogo A. Jurelevicius, Lucy Seldin   +4 more
wiley   +1 more source

Molecular dynamics simulations and fluorescence spectroscopy suggest reduced tryptophan‐to‐heme energy transfer facilitates lignin degradation in ClassII peroxidases

The FEBS Journal, EarlyView.
In lignin peroxidase and versatile peroxidase, low FRET from buried Trp to heme promotes long‐range electron transfer (LRET) from substrate to heme. This efficient electron flow is facilitated by reduced FRET competition. This mechanism likely evolved to enable the effective degradation of bulky, high‐redox‐potential lignin that cannot reach the enzyme'
Yi Ren, Dale Ang, Haluk Ertan, Anne Poljak, Janice R. Aldrich‐Wright, Wallace J. Bridge, Khawar Sohail Siddiqui   +6 more
wiley   +1 more source

Detoxification of 1,1,2-trichloroethane to ethene by desulfitobacterium and identification of its functional reductase gene.

PLoS ONE, 2015
1,1,2-trichloroethane (1,1,2-TCA) has become a common groundwater pollutant due to historically extensive utilization, improper disposal, as well as from incomplete dechlorination of 1,1,2,2-tetrachloroethane.
Siyan Zhao, Chang Ding, Jianzhong He
doaj   +1 more source

Addition of Shewanella oneidensis MR-1 to the Dehalococcoides-containing culture enhances the trichloroethene dechlorination

Environment International, 2019
Dehalococcoides is able to completely dehalogenate tetrachloroethene (PCE) and trichloroethene (TCE) to ethene (ETH). However, the dechlorination efficiency of Dehalococcoides is low and result in the accumulation of toxic intermediates.
Yaru Li, Li-Lian Wen, He-Ping Zhao, Lizhong Zhu   +3 more
doaj   +1 more source