Results 31 to 40 of about 1,120 (114)

The Membrane-Bound C Subunit of Reductive Dehalogenases: Topology Analysis and Reconstitution of the FMN-Binding Domain of PceC

Frontiers in Microbiology, 2018
Organohalide respiration (OHR) is the energy metabolism of anaerobic bacteria able to use halogenated organic compounds as terminal electron acceptors.
Géraldine F. Buttet, Mathilde S. Willemin, Romain Hamelin, Aamani Rupakula, Julien Maillard   +4 more
doaj   +1 more source

Genomic Characteristics Distinguish Geographically Distributed Dehalococcoidia

Frontiers in Microbiology, 2020
Dehalococcoidia (Dia) class microorganisms are frequently found in various pristine and contaminated environments. Metagenome-assembled genomes (MAGs) and single-cell amplified genomes (SAGs) studies have substantially improved the understanding of Dia ...
Yi Yang, Yaozhi Zhang, Yaozhi Zhang, Natalie L. Cápiro, Jun Yan   +4 more
doaj   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Organohalide Respiring Bacteria and Reductive Dehalogenases: Key Tools in Organohalide Bioremediation

Frontiers in Microbiology, 2016
Organohalides are recalcitrant pollutants that have been responsible for substantial contamination of soils and groundwater. Organohalide-respiring bacteria (ORB) provide a potential solution to remediate contaminated sites, through their ability to use ...
Bat-Erdene eJugder, Haluk eErtan, Haluk eErtan, Susanne eBohl, Matthew eLee, Susanne eBohl, Christopher Peter Marquis, Michael eManefield   +7 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Microbial Community Dynamics in Marine Water: Influence of Hydrocarbon Type and Exposure Time

Environmental Toxicology, EarlyView.
ABSTRACT Marine environments are frequently impacted by petroleum‐derived hydrocarbons, which pose ecological risks because of their toxic, mutagenic, and carcinogenic potential. Microorganisms play a crucial role in hydrocarbon degradation, and understanding the influence of time and hydrocarbon type on microbial dynamics helps improve bioremediation ...
Karen C. F. Santaren, Douglas A. Monteiro, Eliene S. Lopes, Diogo A. Jurelevicius, Lucy Seldin   +4 more
wiley   +1 more source

Detoxification of 1,1,2-trichloroethane to ethene by desulfitobacterium and identification of its functional reductase gene.

PLoS ONE, 2015
1,1,2-trichloroethane (1,1,2-TCA) has become a common groundwater pollutant due to historically extensive utilization, improper disposal, as well as from incomplete dechlorination of 1,1,2,2-tetrachloroethane.
Siyan Zhao, Chang Ding, Jianzhong He
doaj   +1 more source

Photocatalytic and Biotechnological Strategies for Remediation of Persistent Organic Pollutants: Mechanisms, Toxicity, and Antimicrobial Perspectives

Environmental Toxicology, EarlyView.
ABSTRACT Persistent organic pollutants (POPs) pose significant ecological and human health risks due to their persistence, bioaccumulation, toxicity, and global distribution. Conventional remediation methods are often inadequate for achieving complete mineralization, demonstrating the need for advanced and substantial approaches.
Viola O. Okechukwu, Patrick B. Njobeh, Jonathan O. Okonkwo   +2 more
wiley   +1 more source

Microbial Community Changes in a Chlorinated Solvents Polluted Aquifer Over the Field Scale Treatment With Poly-3-Hydroxybutyrate as Amendment

Frontiers in Microbiology, 2018
This study investigated the organohalide-respiring bacteria (OHRB) and the supporting microbial populations operating in a pilot scale plant employing poly-3-hydroxybutyrate (PHB), a biodegradable polymer produced by bacteria from waste streams, for the ...
Bruna Matturro, Lucia Pierro, Emanuela Frascadore, Marco Petrangeli Papini, Simona Rossetti   +4 more
doaj   +1 more source

Molecular dynamics simulations and fluorescence spectroscopy suggest reduced tryptophan‐to‐heme energy transfer facilitates lignin degradation in ClassII peroxidases

The FEBS Journal, EarlyView.
In lignin peroxidase and versatile peroxidase, low FRET from buried Trp to heme promotes long‐range electron transfer (LRET) from substrate to heme. This efficient electron flow is facilitated by reduced FRET competition. This mechanism likely evolved to enable the effective degradation of bulky, high‐redox‐potential lignin that cannot reach the enzyme'
Yi Ren, Dale Ang, Haluk Ertan, Anne Poljak, Janice R. Aldrich‐Wright, Wallace J. Bridge, Khawar Sohail Siddiqui   +6 more
wiley   +1 more source