Results 21 to 30 of about 1,120 (114)

Dehalococcoides mccartyi strain CBDB1 takes up protons from the cytoplasm to reductively dehalogenate organohalides indicating a new modus of proton motive force generation

Frontiers in Microbiology, 2023
Proton translocation across the cytoplasmic membrane is a vital process for all organisms. Dehalococcoides strains are strictly anaerobic organohalide respiring bacteria that lack quinones and cytochromes but express a large membrane-bound protein ...
Nadine Hellmold, Marie Eberwein, My Hanh Thi Phan, Steffen Kümmel, Oliver Einsle, Darja Deobald, Lorenz Adrian, Lorenz Adrian   +7 more
doaj   +1 more source

Microbiome Composition and Dynamics of a Reductive/Oxidative Bioelectrochemical System for Perchloroethylene Removal: Effect of the Feeding Composition

Frontiers in Microbiology, 2022
Chlorinated solvents still represent an environmental concern that requires sustainable and innovative bioremediation strategies. This study describes the microbiome composition of a novel bioelectrochemical system (BES) based on sequential reductive ...
Maria L. Di Franca, Bruna Matturro, Simona Crognale, Marco Zeppilli, Edoardo Dell’Armi, Mauro Majone, Marco Petrangeli Papini, Simona Rossetti   +7 more
doaj   +1 more source

The BhbA Enzyme Assay

Bio-Protocol, 2014
Reductive dehalogenation has been found primarily in anaerobic communities and is originally thought to rarely occur in aerobes. A reductive dehalogenase (BhbA) was characterized from an aerobic strain of Comamonas sp.
Kai Chen, Jiandong Jiang
doaj   +1 more source

Genome-Guided Identification of Organohalide-Respiring Deltaproteobacteria from the Marine Environment

mBio, 2018
Organohalide compounds are widespread in the environment as a result of both anthropogenic activities and natural production. The marine environment, in particular, is a major reservoir of organohalides, and reductive dehalogenation is thought to be an ...
Jie Liu, Max M. Häggblom
doaj   +1 more source

The Complexome of Dehalococcoides mccartyi Reveals Its Organohalide Respiration-Complex Is Modular

Frontiers in Microbiology, 2018
Dehalococcoides mccartyi strain CBDB1 is a slow growing strictly anaerobic microorganism dependent on halogenated compounds as terminal electron acceptor for anaerobic respiration.
Katja Seidel, Joana Kühnert, Lorenz Adrian, Lorenz Adrian   +3 more
doaj   +1 more source

Sister Dehalobacter Genomes Reveal Specialization in Organohalide Respiration and Recent Strain Differentiation Likely Driven by Chlorinated Substrates

Frontiers in Microbiology, 2016
The genomes of two closely related Dehalobacter strains (strain CF and strain DCA) were assembled from the metagenome of an anaerobic enrichment culture that reductively dechlorinates chloroform (CF), 1,1,1-trichloroethane (1,1,1-TCA) and 1,1 ...
Shuiquan eTang, Po Hsiang eWang, Steven eHiggins, Steven eHiggins, Frank eLoeffler, Frank eLoeffler, Elizabeth Anne Edwards   +6 more
doaj   +1 more source

Isolation and genomic characterization of a Dehalococcoides strain suggests genomic rearrangement during culture

Scientific Reports, 2017
We have developed and characterized a bacterial consortium that reductively dechlorinates trichloroethene to ethene. Quantitative PCR analysis for the 16S rRNA and reductive dehalogenase genes showed that the consortium is highly enriched with ...
Masafumi Yohda, Kentaro Ikegami, Yuto Aita, Mizuki Kitajima, Ayane Takechi, Megumi Iwamoto, Tomomi Fukuda, Noriyoshi Tamura, Junji Shibasaki, Seiji Koike, Daisuke Komatsu, Sakari Miyagi, Minoru Nishimura, Yoshihito Uchino, Akino Shiroma, Makiko Shimoji, Hinako Tamotsu, Noriko Ashimine, Misuzu Shinzato, Shun Ohki, Kazuma Nakano, Kuniko Teruya, Kazuhito Satou, Takashi Hirano, Osami Yagi   +24 more
doaj   +1 more source

Functional Expression and Characterization of Tetrachloroethene Dehalogenase From Geobacter sp.

Frontiers in Microbiology, 2018
Reductive dehalogenase (RDase) consists of two parts, RdhA and RdhB. RdhA is the catalytic subunit, harboring a cobalamin cofactor and two Fe–S clusters. RdhA is anchored to the cytoplasmic membrane via the membrane anchoring subunit, RdhB.
Ryuki Nakamura, Tomohiro Obata, Ryota Nojima, Yohey Hashimoto, Keiichi Noguchi, Takahiro Ogawa, Masafumi Yohda, Masafumi Yohda   +7 more
doaj   +1 more source

Merging Biocatalysis and Chemocatalysis in Flow: State‐of‐the‐Art and Future Directions for Sustainable Synthesis

Angewandte Chemie, Volume 138, Issue 25, 15 June 2026.
This review highlights recent advances in integrating biocatalysis and chemocatalysis in continuous flow to create streamlined, sustainable processes. It examines chemo‐enzymatic cascades combining at least one enzymatic and one chemical step, discusses challenges such as enzyme immobilization, leaching, and reactor clogging, and presents solutions ...
Petros Siasiaridis, Matteo Damian, Francesco G. Mutti   +2 more
wiley   +2 more sources

Proteomic data set of the organohalide-respiring Epsilonproteobacterium Sulfurospirillum multivorans adapted to tetrachloroethene and other energy substrates

Data in Brief, 2016
Sulfurospirillum multivorans is a free-living, physiologically versatile Epsilonproteobacterium able to couple the reductive dehalogenation of chlorinated and brominated ethenes to growth (organohalide respiration).
Tobias Goris, Christian L. Schiffmann, Jennifer Gadkari, Lorenz Adrian, Martin von Bergen, Gabriele Diekert, Nico Jehmlich   +6 more
doaj   +1 more source