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Catabolic Reductive Dehalogenase Substrate Complex Structures Underpin Rational Repurposing of Substrate Scope [PDF]

open access: yesMicroorganisms, 2020
Reductive dehalogenases are responsible for the reductive cleavage of carbon-halogen bonds during organohalide respiration. A variety of mechanisms have been proposed for these cobalamin and [4Fe-4S] containing enzymes, including organocobalt, radical ...
Stephen E J Rigby   +2 more
exaly   +5 more sources

Presence, Diversity, and Enrichment of Respiratory Reductive Dehalogenase and Non-Respiratory Hydrolytic and Oxidative Dehalogenase Genes in Terrestrial Environments [PDF]

open access: yesFrontiers in Microbiology, 2019
Organohalide-respiring bacteria have been linked to the cycling and possible respiration of chlorinated natural organic matter (Cl-NOM) in uncontaminated soils and sediments. The importance of non-respiratory hydrolytic/oxidative dechlorination processes
Hanna R Temme, Paige J Novak
exaly   +7 more sources

Diversity of Reductive Dehalogenase Genes From Environmental Samples and Enrichment Cultures Identified With Degenerate Primer PCR Screens [PDF]

open access: yesFrontiers in Microbiology, 2013
Reductive dehalogenases are the critical enzymes for anaerobic organohalide respiration, a microbial metabolic process that has been harnessed for bioremediation efforts to resolve chlorinated solvent contamination in groundwater and is implicated in the
Laura A Hug, Elizabeth A. Edwards
exaly   +4 more sources

Distribution of Dehalococcoidia in the Anaerobic Deep Water of a Remote Meromictic Crater Lake and Detection of Dehalococcoidia-Derived Reductive Dehalogenase Homologous Genes. [PDF]

open access: yesPLoS ONE, 2016
Here we describe the natural occurrence of bacteria of the class Dehalococcoidia (DEH) and their diversity at different depths in anoxic waters of a remote meromictic lake (Lake Pavin) using 16S rRNA gene amplicon sequencing and quantitative PCR ...
Corinne Biderre-Petit   +10 more
doaj   +3 more sources

Stoichiometry of the Gene Products From the Tetrachloroethene Reductive Dehalogenase Operon pceABCT [PDF]

open access: yesFrontiers in Microbiology, 2022
Organohalide respiration (OHR) is a bacterial anaerobic process that uses halogenated compounds, e.g., tetrachloroethene (PCE), as terminal electron acceptors.
Lorenzo Cimmino   +3 more
doaj   +6 more sources

Dehalococcoides mccartyi strain CBDB1 takes up protons from the cytoplasm to reductively dehalogenate organohalides indicating a new modus of proton motive force generation

open access: yesFrontiers in Microbiology, 2023
Proton translocation across the cytoplasmic membrane is a vital process for all organisms. Dehalococcoides strains are strictly anaerobic organohalide respiring bacteria that lack quinones and cytochromes but express a large membrane-bound protein ...
Nadine Hellmold   +7 more
doaj   +1 more source

Microbiome Composition and Dynamics of a Reductive/Oxidative Bioelectrochemical System for Perchloroethylene Removal: Effect of the Feeding Composition

open access: yesFrontiers in Microbiology, 2022
Chlorinated solvents still represent an environmental concern that requires sustainable and innovative bioremediation strategies. This study describes the microbiome composition of a novel bioelectrochemical system (BES) based on sequential reductive ...
Maria L. Di Franca   +7 more
doaj   +1 more source

The BhbA Enzyme Assay

open access: yesBio-Protocol, 2014
Reductive dehalogenation has been found primarily in anaerobic communities and is originally thought to rarely occur in aerobes. A reductive dehalogenase (BhbA) was characterized from an aerobic strain of Comamonas sp.
Kai Chen, Jiandong Jiang
doaj   +1 more source

Genome-Guided Identification of Organohalide-Respiring Deltaproteobacteria from the Marine Environment

open access: yesmBio, 2018
Organohalide compounds are widespread in the environment as a result of both anthropogenic activities and natural production. The marine environment, in particular, is a major reservoir of organohalides, and reductive dehalogenation is thought to be an ...
Jie Liu, Max M. Häggblom
doaj   +1 more source

The Complexome of Dehalococcoides mccartyi Reveals Its Organohalide Respiration-Complex Is Modular

open access: yesFrontiers in Microbiology, 2018
Dehalococcoides mccartyi strain CBDB1 is a slow growing strictly anaerobic microorganism dependent on halogenated compounds as terminal electron acceptor for anaerobic respiration.
Katja Seidel   +3 more
doaj   +1 more source

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