Results 11 to 20 of about 83,987 (180)

A bacterial chloroform reductive dehalogenase: purification and biochemical characterization [PDF]

Microbial Biotechnology, 2017
Summary We report herein the purification of a chloroform (CF)‐reducing enzyme, TmrA, from the membrane fraction of a strict anaerobe Dehalobacter sp. strain UNSWDHB to apparent homogeneity with an approximate 23‐fold increase in relative purity compared
Bat‐Erdene Jugder, Susanne Bohl, Helene Lebhar, Robert D. Healey, Mike Manefield, Christopher P. Marquis, Matthew Lee   +6 more
doaj   +6 more sources

Deciphering reductive dehalogenase specificity through targeted mutagenesis of chloroalkane reductases [PDF]

Applied and Environmental Microbiology
Reductive dehalogenases (RDases) are essential in the anaerobic degradation of various organohalide contaminants. This family of enzymes has broad sequence diversity, but high structural conservation. There have been few studies assessing how RDase amino
Katherine J. Picott, Connor M. Bowers, Elizabeth A. Edwards   +2 more
doaj   +4 more sources

NADPH-Driven Organohalide Reduction by a Nonrespiratory Reductive Dehalogenase

Biochemistry, 2018
Reductive dehalogenases are corrinoid and iron–sulfur cluster-dependent enzymes that mostly act as the terminal oxidoreductases in the bacterial organohalide respiration process.
Fraser A. Collins (5388674), David Leys (1328622), Karl Fisher (1740823), Samantha Gaytan Mondragon (5388671), Stephen E. J. Rigby (110349), Karl A. P. Payne (4796952)   +5 more
core   +5 more sources

Heterologous Production and Purification of a Functional Chloroform Reductive Dehalogenase

ACS Chemical Biology, 2018
Reductive dehalogenases (RDases) are key enzymes involved in the respiratory process of anaerobic organohalide respiring bacteria (ORB). Heterologous expression of respiratory RDases is desirable for structural and functional studies; however, there are ...
Susanne Bohl, Marquis, Christopher P., Christopher P. Marquis, Lebhar, Helene, David Leys, Fisher, Karl, Manefield, Mike, Karl Fisher, Matthew Lee, Jugder, Bat Erdene, Payne, Karl A.P., Bohl, Susanne, Leys, David, Bat-Erdene Jugder, Helene Lebhar, Mike Manefield, Karl A. P. Payne, Lee, Matthew   +17 more
core   +5 more sources

Production of Functional Recombinant Chloroform Reductive Dehalogenase [PDF]

, 2021
Organohalides are recalcitrant, toxic environmental pollutants. The presence of these compounds at elevated levels in soil and aquifers has led to a focus on methods to remediate these sites.
Rahmatullah, Rabeya
core   +3 more sources

Functional Genotyping of Sulfurospirillum spp. in Mixed Cultures Allowed the Identification of a New Tetrachloroethene Reductive Dehalogenase [PDF]

Appl Environ Microbiol, 2013
Reductive dehalogenases are the key enzymes involved in the anaerobic respiration of organohalides such as the widespread groundwater pollutant tetrachloroethene.
Holliger, Christof, Maillard, Julien, Buttet, Geraldine F., Maillard J, Buttet GF, Holliger C   +5 more
core   +2 more sources

Dechlorination of mixed chlorinated organophosphate esters (V6 and TCEP) and associated reductive dehalogenase gene expression by Dehalococcoides mccartyi [PDF]

Applied and Environmental Microbiology
Dehalococcoides mccartyi (D. mccartyi) plays a critical role in the dechlorination of halogenated organic pollutants, yet its performance on mixed chlorinated organophosphate esters (Cl-OPEs) remains poorly understood.
Sen Yang, Junhong Wu, Qian Yang, Yirong Deng, Heli Wang, Dan Li, Lihua Yang, Jianzhong Song, Yin Zhong, Ping'an Peng   +9 more
doaj   +2 more sources

Dual Element (C/Cl) Isotope Analysis Indicates Distinct Mechanisms of Reductive Dehalogenation of Chlorinated Ethenes and Dichloroethane in Dehalococcoides mccartyi Strain BTF08 With Defined Reductive Dehalogenase Inventories [PDF]

Frontiers in Microbiology, 2020
Dehalococcoides mccartyi strain BTF08 has the unique property to couple complete dechlorination of tetrachloroethene and 1,2-dichloroethane to ethene with growth by using the halogenated compounds as terminal electron acceptor. The genome of strain BTF08
Steffi Franke, Katja Seidel, Lorenz Adrian, Lorenz Adrian, Ivonne Nijenhuis   +4 more
doaj   +2 more sources

Reductive dechlorination of tetrachloroethene by a stepwise catalysis of different organohalide respiring bacteria and reductive dehalogenases [PDF]

Biodegradation, 2011
The enrichment culture SL2 dechlorinating tetrachloroethene (PCE) to ethene with strong trichloroethene (TCE) accumulation prior to cis-1,2-dichloroethene (cis-DCE) formation was analyzed for the presence of organohalide respiring bacteria and reductive ...
Marie-Paule Charnay, Julien Maillard, Charnay, Marie-Paule, Rouzeau-Szynalski, Katia, Holliger, Christof, Maillard, Julien, Christof Holliger, Rohrbach-Brandt, Emmanuelle, Emmanuelle Rohrbach-Brandt, Pierre Rossi, Regeard, Christophe, Rossi, Pierre, Christophe Regeard, Katia Rouzeau-Szynalski, Rouzeau-Szynalski K, Regeard C, Holliger C, Rossi P, Maillard J, Charnay MP, Rohrbach-Brandt E   +20 more
core   +6 more sources

Reductive Dehalogenases Come of Age in Biological Destruction of Organohalides

Trends in Biotechnology, 2015
Halogenated organic compounds (organohalides) are globally prevalent, recalcitrant toxic, and carcinogenic environmental pollutants. Select microorganisms encode enzymes known as reductive dehalogenases (EC 1.97.1.8) that catalyze reductive ...
Ertan, Haluk, Manefield, Michael, Marquis, Christopher P., Jugder, Bat-Erdene, Lee, Matthew   +4 more
core   +3 more sources