Results 51 to 60 of about 781,120 (260)

In vitro and in silico parameters for precise cgMLST typing of Listeria monocytogenes

open access: yesBMC Genomics, 2022
Background Whole genome sequencing analyzed by core genome multi-locus sequence typing (cgMLST) is widely used in surveillance of the pathogenic bacteria Listeria monocytogenes.
Federica Palma   +11 more
doaj   +1 more source

Epigenetic blind spots – the role of DNA methylation dynamics in stem cell‐based models of embryogenesis

open access: yesFEBS Letters, EarlyView.
Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil   +4 more
wiley   +1 more source

Trait biases in microbial reference genomes

open access: yesScientific Data, 2023
AbstractCommon culturing techniques and priorities bias our discovery towards specific traits that may not be representative of microbial diversity in nature. So far, these biases have not been systematically examined. To address this gap, here we use 116,884 publicly available metagenome-assembled genomes (MAGs, completeness ≥80%) from 203 surveys ...
Sage Albright, Stilianos Louca
openaire   +4 more sources

Molecular evidence of hybridization between pig and human Ascaris indicates an interbred species complex infecting humans

open access: yeseLife, 2020
Human ascariasis is a major neglected tropical disease caused by the nematode Ascaris lumbricoides. We report a 296 megabase (Mb) reference-quality genome comprised of 17,902 protein-coding genes derived from a single, representative Ascaris worm.
Alice Easton   +14 more
doaj   +1 more source

Biophysical approaches for studying viral entry

open access: yesFEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source

Development of SSR molecular markers based on the published genome in seashore paspalum

open access: yesGrass Research
Seashore paspalum (Paspalum vaginatum Swartz) is a widely utilized turfgrass in subtropical and tropical regions. To assist germplasm evaluation and breeding new varieties, a study was carried out to identify Simple Sequence Repeats (SSR) sites in the ...
Qian Chen   +3 more
doaj   +1 more source

Comparative Analysis of Genotyping by Sequencing and Whole-Genome Sequencing Methods in Diversity Studies of Olea europaea L.

open access: yesPlants, 2021
Olive, Olea europaea L., is a tree of great economic and cultural importance in the Mediterranean basin. Thousands of cultivars have been described, of which around 1200 are conserved in the different olive germplasm banks.
James Friel   +4 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

open access: yesFEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace   +6 more
wiley   +1 more source

Refgenie: a reference genome resource manager

open access: yesGigaScience, 2020
Abstract Background Reference genome assemblies are essential for high-throughput sequencing analysis projects. Typically, genome assemblies are stored on disk alongside related resources; e.g., many sequence aligners require the assembly to be indexed.
Michał Stolarczyk   +4 more
openaire   +2 more sources

QTLs Identification for Iron Chlorosis in a Segregating Peach–Almond Progeny Through Double-Digest Sequence-Based Genotyping (SBG)

open access: yesFrontiers in Plant Science, 2022
Linkage maps are highly appreciated tools for cultivar and rootstock breeding programs because they are suitable for genetic and genomic studies. In this study, we report on using sequence-based genotyping (SBG) approach to simultaneously discover and ...
Verónica Guajardo   +6 more
doaj   +1 more source

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