Results 41 to 50 of about 84,271 (219)

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

A Theory of Functional Connections-Based hp-Adaptive Mesh Refinement Algorithm for Solving Hypersensitive Two-Point Boundary-Value Problems

open access: yesMathematics
This manuscript introduces the first hp-adaptive mesh refinement algorithm for the Theory of Functional Connections (TFC) to solve hypersensitive two-point boundary-value problems (TPBVPs).
Kristofer Drozd   +2 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Formation-Constrained Cooperative Localization for UAV Swarms in GNSS-Denied Environments

open access: yesSensors
Cooperative localization is critical for UAV swarm operations in GNSS-denied environments. The backbone-listener scheme, using a small subset of agents as active backbone nodes and others as passive listeners, offers notable advantages in reducing ...
Qin Li   +6 more
doaj   +1 more source

Pathways and pitfalls: a qualitative study of student experiences in biomedical science education

open access: yesFEBS Open Bio, EarlyView.
Biomedical science students from underrepresented backgrounds face barriers including financial strain, disrupted laboratory access and cultural exclusion. Peer networks provide vital support when institutional systems are difficult to navigate. To create inclusive learning environments and achieve academic success, educators should blend active, hands‐
Olivia J. Russell   +8 more
wiley   +1 more source

MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis

open access: yesFEBS Open Bio, EarlyView.
MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss   +7 more
wiley   +1 more source

DSVA: An Iterative Refinement Multi-Agent Framework for NL-to-MTL Translation

open access: yesIEEE Access
Safety-critical cyber-physical systems require rigorously verifiable specifications, yet natural language (NL) requirements introduce ambiguity and defects.
Jinhui Lyu, Ye Wu, Yong Cai
doaj   +1 more source

Dynamic Feasible Region Genetic Algorithm for Optimal Operation of a Multi-Reservoir System

open access: yesEnergies, 2012
Seeking the optimal strategy of a multi-reservoir system is an important approach to develop hydropower energy, in which the Genetic Algorithm (GA) is commonly used as an effective tool. However, when the traditional GA is applied in solving the problem,
Bin Xu   +4 more
doaj   +1 more source

Self‐Regulated Learning Meets AI: Reinterpreting Self‐Regulation, Co‐Regulation, and Socially Shared Regulation in Human–AI Interaction

open access: yesNew Directions for Adult and Continuing Education, EarlyView.
ABSTRACT Advancing artificial intelligence (AI) has transformed learning and work, yet higher education and professional development programs have not systematically equipped learners for AI‐prevalent environments. This lack of preparation creates uncertainty regarding control, responsibility, trust, and accountability.
Moon‐Heum Cho, Jerusalem Merkebu
wiley   +1 more source

An autonomous agentic workflow for clinical detection of cognitive concerns using large language models

open access: yesnpj Digital Medicine
Early detection of cognitive impairment is limited by traditional screening tools and resource constraints. We developed two large language model workflows for identifying cognitive concerns from clinical notes: (1) an expert-driven workflow with ...
Jiazi Tian   +12 more
doaj   +1 more source

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